BRENDA - Enzyme Database

The incorporation of beta-hydroxy fatty acids into a phospholipid of Escherichia coli B

Taylor, S.S.; Heath, E.C.; J. Biol. Chem. 244, 6605-6616 (1969)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.40
additional information
no stimulation by coenzyme A, ATP, GTP, UTP, glucosamine 1-phosphate, UDP-N-acetylglucosamine, Triton X-100, lipid A, alkaline treated lipid A or alkaline treated lipid A annealed with Escherichia coli phospholipids
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.40
particle-bound
-
Escherichia coli
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.40
Mg2+
activation, 0.2 M
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.40
Escherichia coli
-
B
-
2.3.1.40
Escherichia coli B / ATCC 11303
-
B
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.40
partial, solubilization by sonic disruption
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.40
acyl-(acyl-carrier protein) + O-(2-acyl-sn-glycero-3-phospho) ethanolamine
specific for C1-position of glycerol-phosphate backbone, the acyl-component of the acyl-(acyl-carrier-protein) may be 3-hydroxydecanoate, 3-hydroxylaurate, 3-hydroxymyristate and 3-hydroxyoctanoate (tentatively identified)
486910
Escherichia coli
(acyl-carrier protein) + O-(1,2-diacyl-sn-glycero-3-phospho) ethanolamine
-
486910
Escherichia coli
?
2.3.1.40
acyl-(acyl-carrier protein) + O-(2-acyl-sn-glycero-3-phospho) ethanolamine
specific for C1-position of glycerol-phosphate backbone, the acyl-component of the acyl-(acyl-carrier-protein) may be 3-hydroxydecanoate, 3-hydroxylaurate, 3-hydroxymyristate and 3-hydroxyoctanoate (tentatively identified)
486910
Escherichia coli B / ATCC 11303
(acyl-carrier protein) + O-(1,2-diacyl-sn-glycero-3-phospho) ethanolamine
-
486910
Escherichia coli B / ATCC 11303
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.40
37
-
assay at
Escherichia coli
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.1.40
100
-
inactivation after 2-5 min
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.40
7.2
-
-
Escherichia coli
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.40
5
10
about half-maximal activity at pH 5.0, about 60% of maximal activity at pH 10.0
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.40
additional information
no stimulation by coenzyme A, ATP, GTP, UTP, glucosamine 1-phosphate, UDP-N-acetylglucosamine, Triton X-100, lipid A, alkaline treated lipid A or alkaline treated lipid A annealed with Escherichia coli phospholipids
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.40
particle-bound
-
Escherichia coli
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.40
Mg2+
activation, 0.2 M
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.40
partial, solubilization by sonic disruption
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.40
acyl-(acyl-carrier protein) + O-(2-acyl-sn-glycero-3-phospho) ethanolamine
specific for C1-position of glycerol-phosphate backbone, the acyl-component of the acyl-(acyl-carrier-protein) may be 3-hydroxydecanoate, 3-hydroxylaurate, 3-hydroxymyristate and 3-hydroxyoctanoate (tentatively identified)
486910
Escherichia coli
(acyl-carrier protein) + O-(1,2-diacyl-sn-glycero-3-phospho) ethanolamine
-
486910
Escherichia coli
?
2.3.1.40
acyl-(acyl-carrier protein) + O-(2-acyl-sn-glycero-3-phospho) ethanolamine
specific for C1-position of glycerol-phosphate backbone, the acyl-component of the acyl-(acyl-carrier-protein) may be 3-hydroxydecanoate, 3-hydroxylaurate, 3-hydroxymyristate and 3-hydroxyoctanoate (tentatively identified)
486910
Escherichia coli B / ATCC 11303
(acyl-carrier protein) + O-(1,2-diacyl-sn-glycero-3-phospho) ethanolamine
-
486910
Escherichia coli B / ATCC 11303
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.40
37
-
assay at
Escherichia coli
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.1.40
100
-
inactivation after 2-5 min
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.40
7.2
-
-
Escherichia coli
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.40
5
10
about half-maximal activity at pH 5.0, about 60% of maximal activity at pH 10.0
Escherichia coli