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Literature summary extracted from

  • Dreier, J.; Li, Q.; Khosla, C.
    Malonyl-CoA:ACP transacylase from Streptomyces coelicolor has two alternative catalytically active nucleophiles (2001), Biochemistry, 40, 12407-12411.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.39 expression of five mutants in Escherichia coli Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.39 additional information
-
additional information the Km for N-acetylcysteamine is significantly higher than for an acyl-carrier-protein Streptomyces coelicolor
2.3.1.39 0.0208
-
malonyl-CoA wild-type enzyme Streptomyces coelicolor
2.3.1.39 0.0239
-
malonyl-CoA S97A mutant Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Escherichia coli
-
-
-
2.3.1.39 Streptomyces coelicolor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.39
-
Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.39 malonyl-CoA + acyl-carrier protein
-
Escherichia coli CoA + malonyl-[acyl-carrier protein]
-
?
2.3.1.39 malonyl-CoA + acyl-carrier protein
-
Streptomyces coelicolor CoA + malonyl-[acyl-carrier protein]
-
?
2.3.1.39 malonyl-CoA + N-acetylcysteamine
-
Escherichia coli CoA + N-acetyl-S-malonylcysteamine
-
?
2.3.1.39 malonyl-CoA + N-acetylcysteamine in the presence of high N-acetylcysteamine concentrations the forward reaction can be driven to completion Streptomyces coelicolor CoA + N-acetyl-S-malonylcysteamine
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.39 25
-
assay at Escherichia coli
2.3.1.39 25
-
assay at Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.39 0.000933
-
N-acetylcysteamine S97A mutant Streptomyces coelicolor
2.3.1.39 0.00683
-
N-acetylcysteamine N-acetylcysteamine as acceptor thiol, wild-type enzyme Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.39 7.3
-
assay at Streptomyces coelicolor