Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Guerra, D.J.; Ohlrogge, J.B.
    Partial purification and characterization of two forms of malonyl-coenzyme A:acyl carrier protein transacylase from soybean leaf tissue (1986), Arch. Biochem. Biophys., 246, 274-285.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.39 2-mercaptoethanol activation Glycine max
2.3.1.39 malonate at 0.1 mM, MCT1 is doubled in activity and MCT2 is 30-40% than control Glycine max

General Stability

EC Number General Stability Organism
2.3.1.39 freezing leads to 20% loss of isozyme MCT1-activity Glycine max
2.3.1.39 glycerol, 20% v/v, stabilizes isozyme MCT1 Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.39 acetoacetyl-CoA inhibition of both isozymes at 0.02 mM and at 0.1 mM Glycine max
2.3.1.39 CoA not inhibitory Glycine max
2.3.1.39 diethyldicarbonate inhibits MCT1 but not MCT2 at 1 mM Glycine max
2.3.1.39 malonyl-CoA inhibition of MCT1 at 0.01 mM and inhibition of both isozymes at 0.02 mM and 0.04 mM; inhibits both MCT1 and MCT2 Glycine max
2.3.1.39 methylmalonyl-CoA inhibition of MCT1 at 0.1 mM Glycine max
2.3.1.39 additional information pH-dependent inhibition of malonyl-enzyme intermediate formation by N-ethylmaleimide Escherichia coli
2.3.1.39 N-ethylmaleimide
-
 Escherichia coli
2.3.1.39 N-ethylmaleimide 1 mM, isozyme MCT2: 40% loss of activity of isozyme MCT2, no effect on isozyme MCT1, both, MCT1 and MCT2, are inactivated at 10 mM Glycine max
2.3.1.39 phenylmethanesulfonyl fluoride not inhibitory Glycine max
2.3.1.39 propionyl-CoA inhibition of both isozymes at 0.1 mM Glycine max
2.3.1.39 reduced CoA inhibition of both isozymes at 0.1 mM Glycine max
2.3.1.39 succinyl-CoA inhibition of MCT2 at 0.02 mM and inhibition of both isozymes at 0.1 mM Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.39 0.0094
-
 malonyl CoA
-
 Glycine max
2.3.1.39 0.015
-
 malonyl CoA
-
 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.39 43000
-
 HPLC-gel permeation chromatography, 2 isozymic forms HPLC analysis Glycine max

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Escherichia coli
-
-
-
2.3.1.39 Glycine max
-
 soy bean, strain AMSOY71
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.39 of 2 isozymes MCT1 and MCT2, using salt fractionation, ion-exchange chromatography, and chromatofocusing Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.39 leaf isozymes 1 and 2 Glycine max
-
2.3.1.39 seed isozyme 1 is predominant Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.39 0.32
-
 isozyme MCT1 Glycine max
2.3.1.39 1
-
 isozyme MCT2 Glycine max

Storage Stability

EC Number Storage Stability Organism
2.3.1.39 -80°C, stable in the presence of glycerol Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.39 malonyl-CoA + acyl-carrier protein both isozymes may not be completely specific for malonyl-CoA as the substrate Glycine max CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein acetyl-CoA cannot replace malonyl-CoA Escherichia coli CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein acetyl-CoA cannot replace malonyl-CoA Glycine max CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein via stable covalent malonyl-enzyme intermediate Escherichia coli CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein via stable covalent malonyl-enzyme intermediate Glycine max CoA + malonyl-[acyl-carrier protein]
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.39 20
-
 assay at Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.39 50
-
-
 Escherichia coli
2.3.1.39 50
-
 isozyme MCT1: 50% loss of activity after 5 min and 60% loss of activity after 10 min, isozyme MCT2: 30% increase of activity after 10 min Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.39 7.5 8.5
-
 Glycine max
2.3.1.39 8.5
-
-
 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.39 7.5 8.5 maximal activity Glycine max