BRENDA - Enzyme Database

Partial purification and characterization of two forms of malonyl-coenzyme A:acyl carrier protein transacylase from soybean leaf tissue

Guerra, D.J.; Ohlrogge, J.B.; Arch. Biochem. Biophys. 246, 274-285 (1986)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.39
2-mercaptoethanol
activation
Glycine max
2.3.1.39
malonate
at 0.1 mM, MCT1 is doubled in activity and MCT2 is 30-40% than control
Glycine max
General Stability
EC Number
General Stability
Organism
2.3.1.39
freezing leads to 20% loss of isozyme MCT1-activity
Glycine max
2.3.1.39
glycerol, 20% v/v, stabilizes isozyme MCT1
Glycine max
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.39
acetoacetyl-CoA
inhibition of both isozymes at 0.02 mM and at 0.1 mM
Glycine max
2.3.1.39
CoA
not inhibitory
Glycine max
2.3.1.39
diethyldicarbonate
inhibits MCT1 but not MCT2 at 1 mM
Glycine max
2.3.1.39
malonyl-CoA
inhibition of MCT1 at 0.01 mM and inhibition of both isozymes at 0.02 mM and 0.04 mM; inhibits both MCT1 and MCT2
Glycine max
2.3.1.39
methylmalonyl-CoA
inhibition of MCT1 at 0.1 mM
Glycine max
2.3.1.39
additional information
pH-dependent inhibition of malonyl-enzyme intermediate formation by N-ethylmaleimide
Escherichia coli
2.3.1.39
N-ethylmaleimide
-
Escherichia coli
2.3.1.39
N-ethylmaleimide
1 mM, isozyme MCT2: 40% loss of activity of isozyme MCT2, no effect on isozyme MCT1, both, MCT1 and MCT2, are inactivated at 10 mM
Glycine max
2.3.1.39
phenylmethanesulfonyl fluoride
not inhibitory
Glycine max
2.3.1.39
propionyl-CoA
inhibition of both isozymes at 0.1 mM
Glycine max
2.3.1.39
reduced CoA
inhibition of both isozymes at 0.1 mM
Glycine max
2.3.1.39
succinyl-CoA
inhibition of MCT2 at 0.02 mM and inhibition of both isozymes at 0.1 mM
Glycine max
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.39
0.0094
-
malonyl CoA
-
Glycine max
2.3.1.39
0.015
-
malonyl CoA
-
Glycine max
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.39
43000
-
HPLC-gel permeation chromatography, 2 isozymic forms HPLC analysis
Glycine max
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.39
Escherichia coli
-
-
-
2.3.1.39
Glycine max
-
soy bean, strain AMSOY71
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.39
of 2 isozymes MCT1 and MCT2, using salt fractionation, ion-exchange chromatography, and chromatofocusing
Glycine max
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.39
leaf
isozymes 1 and 2
Glycine max
-
2.3.1.39
seed
isozyme 1 is predominant
Glycine max
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.39
0.32
-
isozyme MCT1
Glycine max
2.3.1.39
1
-
isozyme MCT2
Glycine max
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.39
-80°C, stable in the presence of glycerol
Glycine max
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.39
malonyl-CoA + acyl-carrier protein
both isozymes may not be completely specific for malonyl-CoA as the substrate
486887
Glycine max
CoA + malonyl-[acyl-carrier protein]
-
486887
Glycine max
?
2.3.1.39
malonyl-CoA + acyl-carrier protein
acetyl-CoA cannot replace malonyl-CoA
486887
Escherichia coli
CoA + malonyl-[acyl-carrier protein]
-
486887
Escherichia coli
?
2.3.1.39
malonyl-CoA + acyl-carrier protein
acetyl-CoA cannot replace malonyl-CoA
486887
Glycine max
CoA + malonyl-[acyl-carrier protein]
-
486887
Glycine max
?
2.3.1.39
malonyl-CoA + acyl-carrier protein
via stable covalent malonyl-enzyme intermediate
486887
Escherichia coli
CoA + malonyl-[acyl-carrier protein]
-
486887
Escherichia coli
?
2.3.1.39
malonyl-CoA + acyl-carrier protein
via stable covalent malonyl-enzyme intermediate
486887
Glycine max
CoA + malonyl-[acyl-carrier protein]
-
486887
Glycine max
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.39
20
-
assay at
Glycine max
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.1.39
50
-
-
Escherichia coli
2.3.1.39
50
-
isozyme MCT1: 50% loss of activity after 5 min and 60% loss of activity after 10 min, isozyme MCT2: 30% increase of activity after 10 min
Glycine max
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.39
7.5
8.5
-
Glycine max
2.3.1.39
8.5
-
-
Glycine max
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.39
7.5
8.5
maximal activity
Glycine max
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.39
2-mercaptoethanol
activation
Glycine max
2.3.1.39
malonate
at 0.1 mM, MCT1 is doubled in activity and MCT2 is 30-40% than control
Glycine max
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.39
freezing leads to 20% loss of isozyme MCT1-activity
Glycine max
2.3.1.39
glycerol, 20% v/v, stabilizes isozyme MCT1
Glycine max
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.39
acetoacetyl-CoA
inhibition of both isozymes at 0.02 mM and at 0.1 mM
Glycine max
2.3.1.39
CoA
not inhibitory
Glycine max
2.3.1.39
diethyldicarbonate
inhibits MCT1 but not MCT2 at 1 mM
Glycine max
2.3.1.39
malonyl-CoA
inhibition of MCT1 at 0.01 mM and inhibition of both isozymes at 0.02 mM and 0.04 mM; inhibits both MCT1 and MCT2
Glycine max
2.3.1.39
methylmalonyl-CoA
inhibition of MCT1 at 0.1 mM
Glycine max
2.3.1.39
additional information
pH-dependent inhibition of malonyl-enzyme intermediate formation by N-ethylmaleimide
Escherichia coli
2.3.1.39
N-ethylmaleimide
-
Escherichia coli
2.3.1.39
N-ethylmaleimide
1 mM, isozyme MCT2: 40% loss of activity of isozyme MCT2, no effect on isozyme MCT1, both, MCT1 and MCT2, are inactivated at 10 mM
Glycine max
2.3.1.39
phenylmethanesulfonyl fluoride
not inhibitory
Glycine max
2.3.1.39
propionyl-CoA
inhibition of both isozymes at 0.1 mM
Glycine max
2.3.1.39
reduced CoA
inhibition of both isozymes at 0.1 mM
Glycine max
2.3.1.39
succinyl-CoA
inhibition of MCT2 at 0.02 mM and inhibition of both isozymes at 0.1 mM
Glycine max
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.39
0.0094
-
malonyl CoA
-
Glycine max
2.3.1.39
0.015
-
malonyl CoA
-
Glycine max
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.39
43000
-
HPLC-gel permeation chromatography, 2 isozymic forms HPLC analysis
Glycine max
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.39
of 2 isozymes MCT1 and MCT2, using salt fractionation, ion-exchange chromatography, and chromatofocusing
Glycine max
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.39
leaf
isozymes 1 and 2
Glycine max
-
2.3.1.39
seed
isozyme 1 is predominant
Glycine max
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.39
0.32
-
isozyme MCT1
Glycine max
2.3.1.39
1
-
isozyme MCT2
Glycine max
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.39
-80°C, stable in the presence of glycerol
Glycine max
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.39
malonyl-CoA + acyl-carrier protein
both isozymes may not be completely specific for malonyl-CoA as the substrate
486887
Glycine max
CoA + malonyl-[acyl-carrier protein]
-
486887
Glycine max
?
2.3.1.39
malonyl-CoA + acyl-carrier protein
acetyl-CoA cannot replace malonyl-CoA
486887
Escherichia coli
CoA + malonyl-[acyl-carrier protein]
-
486887
Escherichia coli
?
2.3.1.39
malonyl-CoA + acyl-carrier protein
acetyl-CoA cannot replace malonyl-CoA
486887
Glycine max
CoA + malonyl-[acyl-carrier protein]
-
486887
Glycine max
?
2.3.1.39
malonyl-CoA + acyl-carrier protein
via stable covalent malonyl-enzyme intermediate
486887
Escherichia coli
CoA + malonyl-[acyl-carrier protein]
-
486887
Escherichia coli
?
2.3.1.39
malonyl-CoA + acyl-carrier protein
via stable covalent malonyl-enzyme intermediate
486887
Glycine max
CoA + malonyl-[acyl-carrier protein]
-
486887
Glycine max
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.39
20
-
assay at
Glycine max
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.1.39
50
-
-
Escherichia coli
2.3.1.39
50
-
isozyme MCT1: 50% loss of activity after 5 min and 60% loss of activity after 10 min, isozyme MCT2: 30% increase of activity after 10 min
Glycine max
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.39
7.5
8.5
-
Glycine max
2.3.1.39
8.5
-
-
Glycine max
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.39
7.5
8.5
maximal activity
Glycine max