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Literature summary extracted from

  • Alberts, A.W.; Majerus, P.W.; Vagelos, P.R.
    Malonyl-CoA acyl carrier protein transacylase (1969), Methods Enzymol., 14, 53-56.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.39 iodoacetamide 59% inhibition at 0.1 mM Escherichia coli
2.3.1.39 N-ethylmaleimide 79% inhibition at 0.1 mM Escherichia coli
2.3.1.39 sulfhydryl compounds
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.39 cytoplasm
-
 Escherichia coli 5737
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.39 using streptomycin sulfate, column chromatography on DEAE-cellulose, ammonium sulfate fractionation, chromatography on Sephadex G-100 I column, DEAE-Sephadex column and Sephadex G-100 II column Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.39 7.04
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.3.1.39 -20°C, 2-4 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.39 malonyl-CoA + acyl-carrier protein acetyl-CoA cannot replace malonyl-CoA Escherichia coli CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein the enzyme is very specific for the malonyl group Escherichia coli CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + pantetheine
-
 Escherichia coli malonyl-pantetheine + CoA
-
 ?
2.3.1.39 malonyl-pantetheine + acyl-carrier protein
-
 Escherichia coli pantetheine + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 additional information mercaptoethanol is inactive as a substitute for acyl-carrier-protein Escherichia coli ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.39 30
-
 assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.39 additional information
-
 the enzyme is quite stable to heat Escherichia coli
2.3.1.39 80
-
 20% loss of activity after 20 min Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.39 6.5
-
-
 Escherichia coli