EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.9 | expression in Escherichia coli | Streptomyces collinus |
2.3.1.38 | cloning and sequencing of fadA gene, expression in Escherichia coli of wild-type and C92S mutant | Streptomyces collinus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.9 | C92S | kcat decreases to 0.2% of that for the recombinant thiolase | Streptomyces collinus |
2.3.1.38 | C92S | no remaining activity acetyl-CoA:acyl carrier protein transacylase activity, but remaining strongly reduced thiolase activity | Streptomyces collinus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.9 | thiolactomycin | 0.36 mM, 50% inhibition | Streptomyces collinus | |
2.3.1.38 | iodoacetamide | acetyl-CoA protects; strong | Streptomyces collinus | |
2.3.1.38 | thiolactomycin | - |
Streptomyces collinus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.9 | 0.0038 | - |
acetoacetyl-CoA | native thiolase | Streptomyces collinus | |
2.3.1.9 | 0.0062 | - |
acetoacetyl-CoA | C92S mutant thiolase | Streptomyces collinus | |
2.3.1.9 | 0.0098 | - |
acetoacetyl-CoA | recombinant thiolase | Streptomyces collinus | |
2.3.1.38 | 0.0043 | - |
acetyl-CoA | native wild-type enzyme | Streptomyces collinus | |
2.3.1.38 | 0.0087 | - |
acetyl-CoA | recombinant wild-type enzyme | Streptomyces collinus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.9 | 185000 | - |
native thiolase, gel filtration | Streptomyces collinus |
2.3.1.9 | 187000 | - |
recombinant thiolase, gel filtration | Streptomyces collinus |
2.3.1.38 | additional information | - |
amino acid sequence, comparison | Streptomyces collinus |
2.3.1.38 | 45000 | - |
4 * 45000, SDS-PAGE | Streptomyces collinus |
2.3.1.38 | 185000 | - |
gel filtration | Streptomyces collinus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.9 | CoA + acetoacetyl-CoA | Streptomyces collinus | - |
acetyl-CoA + acetyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.9 | Streptomyces collinus | - |
- |
- |
2.3.1.38 | Streptomyces collinus | - |
gene fadA | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.9 | Q-Sepharose, ammonium sulfate, Sephacryl S-200, Mono Q, FPLC S-300 | Streptomyces collinus |
2.3.1.38 | native wild-type and recombinant wild-type and mutant from E. coli | Streptomyces collinus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.9 | 36.6 | - |
- |
Streptomyces collinus |
2.3.1.38 | 0.022 | - |
purified native wild-type enzyme | Streptomyces collinus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.9 | CoA + acetoacetyl-CoA | - |
Streptomyces collinus | acetyl-CoA + acetyl-CoA | - |
? | |
2.3.1.9 | CoA + acetoacetyl-CoA | enzyme has both thiolase and acetyl-CoA:acyl carrier protein transacylase activity | Streptomyces collinus | acetyl-CoA + acetyl-CoA | - |
r | |
2.3.1.38 | acetyl-CoA + [acyl-carrier protein] | specific for acetyl-CoA, utilizes [acyl-carrier-protein] of diverse origins with differing affinities | Streptomyces collinus | CoA + acetyl-[acyl-carrier protein] | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.9 | tetramer | 4 * 46000-48000, recombinant thiolase, SDS-PAGE | Streptomyces collinus |
2.3.1.9 | tetramer | 4 * 45000-47000, native thiolase, SDS-PAGE | Streptomyces collinus |
2.3.1.38 | tetramer | 4 * 45000, SDS-PAGE | Streptomyces collinus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.38 | acetyl-CoA:acyl carrier protein transacylase | - |
Streptomyces collinus |
2.3.1.38 | ACT | - |
Streptomyces collinus |
2.3.1.38 | More | cf. EC 2.3.1.85 and EC 2.3.1.86 | Streptomyces collinus |
2.3.1.38 | More | enzyme has both a acetyl-CoA:acyl carrier protein transacylase activity and a much higher thiolase activity | Streptomyces collinus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.38 | 30 | - |
assay at | Streptomyces collinus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.9 | 60 | - |
15 min, loss of 47% activity | Streptomyces collinus |
2.3.1.38 | additional information | - |
- |
Streptomyces collinus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.9 | 0.0767 | - |
acetoacetyl-CoA | C92S mutant thiolase | Streptomyces collinus | |
2.3.1.9 | 36.2 | - |
acetoacetyl-CoA | native thiolase | Streptomyces collinus | |
2.3.1.9 | 40.1 | - |
acetoacetyl-CoA | recombinant thiolase | Streptomyces collinus | |
2.3.1.38 | 0.0225 | - |
acetyl-CoA | recombinant wild-type, crude Streptomyces glaucescens [acyl-carrier-protein] | Streptomyces collinus | |
2.3.1.38 | 0.0508 | - |
acetyl-CoA | native wild-type, crude E. coli [acyl-carrier-protein] | Streptomyces collinus |