BRENDA - Enzyme Database show

Purification of plant acetyl-CoA: acyl carrier protein transacylase

Shimakata, T.; Stumpf, P.K.; Methods Enzymol. 122, 53-59 (1986)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.38
arsenite
-
Spinacia oleracea
2.3.1.38
N-ethylmaleimide
-
Spinacia oleracea
2.3.1.38
PCMB
-
Spinacia oleracea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.38
0.008
-
acetyl-CoA
-
Spinacia oleracea
2.3.1.38
0.0083
-
butyryl-CoA
-
Spinacia oleracea
2.3.1.38
0.0086
-
hexanoyl-CoA
-
Spinacia oleracea
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.38
48000
-
gel filtration
Spinacia oleracea
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.38
acetyl-CoA + [acyl-carrier protein]
Spinacia oleracea
rate-limiting step of fatty acid synthesis
CoA + acetyl-[acyl-carrier protein]
-
Spinacia oleracea
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.38
Spinacia oleracea
-
var. Viroflay
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.38
partial
Spinacia oleracea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.38
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.38
0.0056
-
partially purified enzyme
Spinacia oleracea
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.38
-70°C, several months
Spinacia oleracea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.38
acetyl-CoA + [acyl-carrier protein]
-
486866
Spinacia oleracea
CoA + acetyl-[acyl-carrier protein]
-
486866
Spinacia oleracea
?
2.3.1.38
acetyl-CoA + [acyl-carrier protein]
rate-limiting step of fatty acid synthesis
486866
Spinacia oleracea
CoA + acetyl-[acyl-carrier protein]
-
486866
Spinacia oleracea
?
2.3.1.38
butyryl-CoA + [acyl-carrier protein]
35% activity of that with acetyl-CoA
486866
Spinacia oleracea
CoA + butyryl-[acyl-carrier protein]
-
-
-
?
2.3.1.38
hexanoyl-CoA + [acyl-carrier protein]
18% activity of that with acetyl-CoA
486866
Spinacia oleracea
CoA + hexanoyl-[acyl-carrier protein]
-
-
-
?
2.3.1.38
additional information
no substrate: malonyl-CoA
486866
Spinacia oleracea
?
-
-
-
-
2.3.1.38
octanoyl-CoA + [acyl-carrier protein]
poor substrate
486866
Spinacia oleracea
CoA + octanoyl-[acyl-carrier protein]
-
-
-
?
2.3.1.38
octanoyl-CoA + [acyl-carrier protein]
7% activity of that with acetyl-CoA
486866
Spinacia oleracea
CoA + octanoyl-[acyl-carrier protein]
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.38
38
-
assay at
Spinacia oleracea
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.38
8.1
-
-
Spinacia oleracea
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.38
7.5
9.1
about half-maximal activity at pH 7.5 and about 75% of maximal activity at pH 9.1
Spinacia oleracea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.38
arsenite
-
Spinacia oleracea
2.3.1.38
N-ethylmaleimide
-
Spinacia oleracea
2.3.1.38
PCMB
-
Spinacia oleracea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.38
0.008
-
acetyl-CoA
-
Spinacia oleracea
2.3.1.38
0.0083
-
butyryl-CoA
-
Spinacia oleracea
2.3.1.38
0.0086
-
hexanoyl-CoA
-
Spinacia oleracea
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.38
48000
-
gel filtration
Spinacia oleracea
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.38
acetyl-CoA + [acyl-carrier protein]
Spinacia oleracea
rate-limiting step of fatty acid synthesis
CoA + acetyl-[acyl-carrier protein]
-
Spinacia oleracea
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.38
partial
Spinacia oleracea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.38
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.38
0.0056
-
partially purified enzyme
Spinacia oleracea
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.38
-70°C, several months
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.38
acetyl-CoA + [acyl-carrier protein]
-
486866
Spinacia oleracea
CoA + acetyl-[acyl-carrier protein]
-
486866
Spinacia oleracea
?
2.3.1.38
acetyl-CoA + [acyl-carrier protein]
rate-limiting step of fatty acid synthesis
486866
Spinacia oleracea
CoA + acetyl-[acyl-carrier protein]
-
486866
Spinacia oleracea
?
2.3.1.38
butyryl-CoA + [acyl-carrier protein]
35% activity of that with acetyl-CoA
486866
Spinacia oleracea
CoA + butyryl-[acyl-carrier protein]
-
-
-
?
2.3.1.38
hexanoyl-CoA + [acyl-carrier protein]
18% activity of that with acetyl-CoA
486866
Spinacia oleracea
CoA + hexanoyl-[acyl-carrier protein]
-
-
-
?
2.3.1.38
additional information
no substrate: malonyl-CoA
486866
Spinacia oleracea
?
-
-
-
-
2.3.1.38
octanoyl-CoA + [acyl-carrier protein]
poor substrate
486866
Spinacia oleracea
CoA + octanoyl-[acyl-carrier protein]
-
-
-
?
2.3.1.38
octanoyl-CoA + [acyl-carrier protein]
7% activity of that with acetyl-CoA
486866
Spinacia oleracea
CoA + octanoyl-[acyl-carrier protein]
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.38
38
-
assay at
Spinacia oleracea
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.38
8.1
-
-
Spinacia oleracea
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.38
7.5
9.1
about half-maximal activity at pH 7.5 and about 75% of maximal activity at pH 9.1
Spinacia oleracea