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Literature summary extracted from

  • Tan, D.; Harrison, T.; Hunter, G.A.; Ferreira, G.C.
    Role of arginine 439 in substrate binding of 5-aminolevulinate synthase (1998), Biochemistry, 37, 1478-1484.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.37 overexpression of His-tagged wild-type and mutants in Escherichia coli Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.37 R433K active site mutant, 2fold increased activity Mus musculus
2.3.1.37 R433L active site mutant, similar to the wild-type Mus musculus
2.3.1.37 R439K active site mutant, 77% activity compared to the wild-type, 9-13fold increased Km for both substrates, 5fold increased dissociation constant for glycine Mus musculus
2.3.1.37 R439L active site mutant, no activity, 30fold increased dissociation constant for glycine Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.37 0.002
-
succinyl-CoA recombinant erythroid isoform wild-type Mus musculus
2.3.1.37 0.0022
-
succinyl-CoA recombinant erythroid isoform mutant R433K Mus musculus
2.3.1.37 0.0032
-
succinyl-CoA recombinant erythroid isoform mutant R433L Mus musculus
2.3.1.37 0.027
-
succinyl-CoA recombinant erythroid isoform mutant R439K Mus musculus
2.3.1.37 11.7
-
glycine recombinant erythroid isoform Mus musculus
2.3.1.37 14.4
-
glycine recombinant erythroid isoform mutant R433K Mus musculus
2.3.1.37 18.4
-
glycine recombinant erythroid isoform mutant R433L Mus musculus
2.3.1.37 103
-
glycine recombinant erythroid isoform mutant R439K Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.37 Mus musculus
-
erythroid-specific isoform
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.37 succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 Tyr121, Asp279, Arg439, and Lys313 are involved in substrate and cofactor binding, mechanism, subunit localisation Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.37 succinyl-CoA + glycine
-
Mus musculus 5-aminolevulinate + CoA + CO2
-
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Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.37 0.508
-
succinyl-CoA recombinant erythroid mutant R439K Mus musculus
2.3.1.37 0.658
-
succinyl-CoA recombinant erythroid isoform Mus musculus
2.3.1.37 0.842
-
succinyl-CoA recombinant erythroid mutant R433L Mus musculus
2.3.1.37 1.35
-
succinyl-CoA recombinant erythroid mutant R433K Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.37 pyridoxal 5'-phosphate required Mus musculus