EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.37 | overexpression of His-tagged wild-type and mutants in Escherichia coli | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.37 | Y121F | exchange mutant of potential cofactor binding residue Tyr121, 5% activity compared to the wild-type, Km for glycine is 5fold increased, lower affinity for pyridoxal 5'phosphate | Mus musculus |
2.3.1.37 | Y121H | exchange mutant of potential cofactor binding residue Tyr121, 36% activity compared to the wild-type, Km for glycine is 34fold increased, lower affinity for pyridoxal 5'phosphate | Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.37 | 0.0016 | - |
succinyl-CoA | recombinant mutant Y121H | Mus musculus | |
2.3.1.37 | 0.002 | - |
succinyl-CoA | recombinant erythroid isoform wild-type | Mus musculus | |
2.3.1.37 | 0.014 | - |
succinyl-CoA | recombinant mutant Y121F | Mus musculus | |
2.3.1.37 | 11.7 | - |
glycine | recombinant erythroid isoform | Mus musculus | |
2.3.1.37 | 52.2 | - |
glycine | recombinant mutant Y121H | Mus musculus | |
2.3.1.37 | 400 | - |
glycine | recombinant mutant Y121F | Mus musculus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.37 | additional information | - |
amino acid sequence alignment | Mus musculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.37 | Mus musculus | - |
erythroid-specific isoform | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.37 | recombinant of His-tagged wild-type and mutants from E. coli | Mus musculus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.37 | succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 | Tyr121, Asp279, Arg439, and Lys313 are involved in substrate and cofactor binding, mechanism, subunit localisation | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.37 | succinyl-CoA + glycine | - |
Mus musculus | 5-aminolevulinate + CoA + CO2 | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.37 | 0.0355 | - |
succinyl-CoA | recombinant erythroid isoform mutant Y121H | Mus musculus | |
2.3.1.37 | 0.235 | - |
succinyl-CoA | recombinant erythroid isoform mutant Y121F | Mus musculus | |
2.3.1.37 | 0.658 | - |
succinyl-CoA | recombinant erythroid isoform | Mus musculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.37 | 7.5 | - |
- |
Mus musculus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.37 | 6.5 | 9.5 | - |
Mus musculus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.37 | pyridoxal 5'-phosphate | required | Mus musculus |