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Literature summary extracted from

  • Tan, D.; Barber, M.J.; Ferreira, G.C.
    The role of tyrosine 121 in cofactor binding of 5-aminolevulinate synthase (1998), Protein Sci., 7, 1208-1213.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.37 overexpression of His-tagged wild-type and mutants in Escherichia coli Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.37 Y121F exchange mutant of potential cofactor binding residue Tyr121, 5% activity compared to the wild-type, Km for glycine is 5fold increased, lower affinity for pyridoxal 5'phosphate Mus musculus
2.3.1.37 Y121H exchange mutant of potential cofactor binding residue Tyr121, 36% activity compared to the wild-type, Km for glycine is 34fold increased, lower affinity for pyridoxal 5'phosphate Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.37 0.0016
-
 succinyl-CoA recombinant mutant Y121H Mus musculus
2.3.1.37 0.002
-
 succinyl-CoA recombinant erythroid isoform wild-type Mus musculus
2.3.1.37 0.014
-
 succinyl-CoA recombinant mutant Y121F Mus musculus
2.3.1.37 11.7
-
 glycine recombinant erythroid isoform Mus musculus
2.3.1.37 52.2
-
 glycine recombinant mutant Y121H Mus musculus
2.3.1.37 400
-
 glycine recombinant mutant Y121F Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.37 additional information
-
 amino acid sequence alignment Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.37 Mus musculus
-
 erythroid-specific isoform
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.37 recombinant of His-tagged wild-type and mutants from E. coli Mus musculus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.37 succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 Tyr121, Asp279, Arg439, and Lys313 are involved in substrate and cofactor binding, mechanism, subunit localisation Mus musculus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.37 succinyl-CoA + glycine
-
 Mus musculus 5-aminolevulinate + CoA + CO2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.37 0.0355
-
 succinyl-CoA recombinant erythroid isoform mutant Y121H Mus musculus
2.3.1.37 0.235
-
 succinyl-CoA recombinant erythroid isoform mutant Y121F Mus musculus
2.3.1.37 0.658
-
 succinyl-CoA recombinant erythroid isoform Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.37 7.5
-
-
 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.37 6.5 9.5
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.37 pyridoxal 5'-phosphate required Mus musculus