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Literature summary extracted from

  • Dailey, H.A.; Dailey, T.A.
    Expression and purification of mammalian 5-aminolevulinate synthase (1997), Methods Enzymol., 281, 336-340.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.37 short half-life Gallus gallus
2.3.1.37 short half-life Mus musculus
2.3.1.37 short half-life Homo sapiens
2.3.1.37 short half-life Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.37 protoheme feed-back inhibition, non-specific isoform ALAS-1 Gallus gallus
2.3.1.37 protoheme feed-back inhibition, non-specific isoform ALAS-1 Homo sapiens
2.3.1.37 protoheme feed-back inhibition, non-specific isoform ALAS-1 Mus musculus
2.3.1.37 protoheme feed-back inhibition, non-specific isoform ALAS-1 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.37 0.055
-
 succinyl-CoA
-
 Mus musculus
2.3.1.37 51
-
 glycine
-
 Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.37 Iron regulation of erythroid-specific isoform ALAS-2 via 5'-iron responsive element, i.e. IRE Gallus gallus
2.3.1.37 Iron regulation of erythroid-specific isoform ALAS-2 via 5'-iron responsive element, i.e. IRE Mus musculus
2.3.1.37 Iron regulation of erythroid-specific isoform ALAS-2 via 5'-iron responsive element, i.e. IRE Homo sapiens
2.3.1.37 Iron regulation of erythroid-specific isoform ALAS-2 via 5'-iron responsive element, i.e. IRE Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.37 56000
-
 2 * 56000, SDS-PAGE Mus musculus
2.3.1.37 112000
-
 gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.37 additional information Homo sapiens enzyme deficiency causes X-linked sideroblastic anemia ?
-
 ?
2.3.1.37 succinyl-CoA + glycine Gallus gallus key enzyme in tetrapyrrole biosynthesis 5-aminolevulinate + CoA + CO2
-
 ?
2.3.1.37 succinyl-CoA + glycine Mus musculus key enzyme in tetrapyrrole biosynthesis 5-aminolevulinate + CoA + CO2
-
 ?
2.3.1.37 succinyl-CoA + glycine Homo sapiens key enzyme in tetrapyrrole biosynthesis 5-aminolevulinate + CoA + CO2
-
 ?
2.3.1.37 succinyl-CoA + glycine Rattus norvegicus key enzyme in tetrapyrrole biosynthesis 5-aminolevulinate + CoA + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.37 Gallus gallus
-
 2 isoforms ALAS-1 and ALAS-2, a houskeeping form and an erythroid-specific form
-
2.3.1.37 Homo sapiens
-
 2 isoforms ALAS-1 and ALAS-2, a houskeeping form and an erythroid-specific form
-
2.3.1.37 Mus musculus
-
 2 isoforms ALAS-1 and ALAS-2, a houskeeping form and an erythroid-specific form
-
2.3.1.37 Rattus norvegicus
-
 non-specific, housekeeping isoform ALAS-N and erythroid-specific isoform ALAS-E
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.37 recombinant from E. coli Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.37 additional information enzyme is synthezised in the cytosol as the precursor protein and then imported into the mitochondria matrix and cleaved to the mature enzyme, the targeting information is encoded in nonoverlapping regions of the presequence Gallus gallus
-
2.3.1.37 additional information enzyme is synthezised in the cytosol as the precursor protein and then imported into the mitochondria matrix and cleaved to the mature enzyme, the targeting information is encoded in nonoverlapping regions of the presequence Mus musculus
-
2.3.1.37 additional information enzyme is synthezised in the cytosol as the precursor protein and then imported into the mitochondria matrix and cleaved to the mature enzyme, the targeting information is encoded in nonoverlapping regions of the presequence Homo sapiens
-
2.3.1.37 additional information enzyme is synthezised in the cytosol as the precursor protein and then imported into the mitochondria matrix and cleaved to the mature enzyme, the targeting information is encoded in nonoverlapping regions of the presequence Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.37 additional information enzyme deficiency causes X-linked sideroblastic anemia Homo sapiens ?
-
 ?
2.3.1.37 succinyl-CoA + glycine
-
 Gallus gallus 5-aminolevulinate + CoA + CO2
-
 ?
2.3.1.37 succinyl-CoA + glycine
-
 Mus musculus 5-aminolevulinate + CoA + CO2
-
 ?
2.3.1.37 succinyl-CoA + glycine
-
 Homo sapiens 5-aminolevulinate + CoA + CO2
-
 ?
2.3.1.37 succinyl-CoA + glycine
-
 Rattus norvegicus 5-aminolevulinate + CoA + CO2
-
 ?
2.3.1.37 succinyl-CoA + glycine key enzyme in tetrapyrrole biosynthesis Gallus gallus 5-aminolevulinate + CoA + CO2
-
 ?
2.3.1.37 succinyl-CoA + glycine key enzyme in tetrapyrrole biosynthesis Mus musculus 5-aminolevulinate + CoA + CO2
-
 ?
2.3.1.37 succinyl-CoA + glycine key enzyme in tetrapyrrole biosynthesis Homo sapiens 5-aminolevulinate + CoA + CO2
-
 ?
2.3.1.37 succinyl-CoA + glycine key enzyme in tetrapyrrole biosynthesis Rattus norvegicus 5-aminolevulinate + CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.37 dimer
-
 Gallus gallus
2.3.1.37 dimer
-
 Rattus norvegicus
2.3.1.37 dimer 2 * 56000, SDS-PAGE Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.37 0.0095
-
 succinyl-CoA
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.37 pyridoxal 5'-phosphate required Gallus gallus
2.3.1.37 pyridoxal 5'-phosphate required Mus musculus
2.3.1.37 pyridoxal 5'-phosphate required Homo sapiens
2.3.1.37 pyridoxal 5'-phosphate required Rattus norvegicus
2.3.1.37 pyridoxal 5'-phosphate noncovalently bound Mus musculus