EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.37 | expression of His-tagged wild-type, mutant K313A, mutant R149A and dimer mutant K313A/R149A, each subunit from 1 plasmid, in Escherichia coli hemA- mutant | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.37 | C145R | mutant strain G205, lacks enzyme activity, but can complement mutant strain Ole3 with mutation G344C | Saccharomyces cerevisiae |
2.3.1.37 | K313A | mutation site located at the active site of 1 subunit, functional complementation of Escherichia coli mutant strain hemA-, no activity | Mus musculus |
2.3.1.37 | K313A/R149A | each mutation site located on 1 subunit, 2 plasmids, coexpression of the dimer in Escherichia coli hemA-, functional complementation, 26% activity compared to wild-type | Mus musculus |
2.3.1.37 | N157Y/N162S | mutant strain G101, lacks enzyme activity, but can complement mutant strain G220 with mutation T452R | Saccharomyces cerevisiae |
2.3.1.37 | R149A | mutation site located at the active site of 1 subunit, functional complementation of Escherichia coli mutant strain hemA-, no activity | Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.37 | 1.52 | - |
succinyl-CoA | recombinant mutant dimer K149A/K313A | Mus musculus | |
2.3.1.37 | 1.82 | - |
succinyl-CoA | - |
Mus musculus | |
2.3.1.37 | 6.95 | - |
glycine | recombinant mutant dimer K149A/K313A | Mus musculus | |
2.3.1.37 | 8.39 | - |
glycine | - |
Mus musculus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.37 | additional information | - |
amino acid sequence alignment | Mus musculus |
2.3.1.37 | additional information | - |
amino acid sequence alignment | Saccharomyces cerevisiae |
2.3.1.37 | 56000 | - |
2 * 56000, SDS-PAGE | Mus musculus |
2.3.1.37 | 112000 | - |
gel filtration | Mus musculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.37 | succinyl-CoA + glycine | Mus musculus | rate-limiting enzyme of heme biosynthesis | 5-aminolevulinate + CoA + CO2 | - |
? | |
2.3.1.37 | succinyl-CoA + glycine | Saccharomyces cerevisiae | rate-limiting enzyme of heme biosynthesis | 5-aminolevulinate + CoA + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.37 | Mus musculus | - |
gene hemA | - |
2.3.1.37 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.37 | recombinant of His-tagged wild-type and mutants from E. coli | Mus musculus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.37 | succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 | Tyr121, Asp279, Arg439, and Lys313 are involved in substrate and cofactor binding, mechanism, subunit localisation | Mus musculus | |
2.3.1.37 | succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 | the active site is located at the subunit interface and contains catalytically essential residues from the two subunits | Mus musculus | |
2.3.1.37 | succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 | the active site is located at the subunit interface and contains catalytically essential residues from the two subunits | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.37 | succinyl-CoA + glycine | - |
Mus musculus | 5-aminolevulinate + CoA + CO2 | - |
? | |
2.3.1.37 | succinyl-CoA + glycine | - |
Saccharomyces cerevisiae | 5-aminolevulinate + CoA + CO2 | - |
? | |
2.3.1.37 | succinyl-CoA + glycine | rate-limiting enzyme of heme biosynthesis | Mus musculus | 5-aminolevulinate + CoA + CO2 | - |
? | |
2.3.1.37 | succinyl-CoA + glycine | rate-limiting enzyme of heme biosynthesis | Saccharomyces cerevisiae | 5-aminolevulinate + CoA + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.37 | dimer | - |
Saccharomyces cerevisiae |
2.3.1.37 | dimer | 2 * 56000, SDS-PAGE | Mus musculus |
2.3.1.37 | More | the active site is located at the subunit interface and contains catalytically essential residues from the two subunits | Mus musculus |
2.3.1.37 | More | the active site is located at the subunit interface and contains catalytically essential residues from the two subunits | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.37 | pyridoxal 5'-phosphate | - |
Mus musculus | |
2.3.1.37 | pyridoxal 5'-phosphate | - |
Saccharomyces cerevisiae |