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Literature summary extracted from

  • Whiting, M.J.; Granick, S.
    delta-Aminolevulinic acid synthase from chick embryo liver mitochondria. I. Purification and some properties (1976), J. Biol. Chem., 251, 1340-1346.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.37 divalent cations Mg2+ or Ca2+: optimal at concentration of 50 mM, inhibition above 200 mM Gallus gallus
2.3.1.37 hemin
-
 Gallus gallus
2.3.1.37 monovalent cations Na+ or Li+: optimal at 0.5 M, not inhibitory up to 1 M Gallus gallus
2.3.1.37 p-chloromercuribenzoate
-
 Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.37 Ca2+ optimal at concentration of 50 mM, inhibition above 200 mM Gallus gallus
2.3.1.37 Li+ optimal at 0.5 M, not inhibitory up to 1 M Gallus gallus
2.3.1.37 Li+ in Mg2+-containing medium: at 100 mM no effect on activity, higher concentrations are totally inhibitory Gallus gallus
2.3.1.37 Mg2+ optimal at concentration of 50 mM, inhibition above 200 mM Gallus gallus
2.3.1.37 Na+ optimal at 0.5 M, not inhibitory up to 1 M Gallus gallus
2.3.1.37 Na+ in Mg2+-containing medium: at 100 mM no effect on activity, higher concentrations are totally inhibitory Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.37 49000
-
 2 * 49000, SDS-PAGE Gallus gallus
2.3.1.37 87000
-
 gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.37 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.37
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.37 embryo
-
 Gallus gallus
-
2.3.1.37 liver fetal Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.37 additional information
-
-
 Gallus gallus

Storage Stability

EC Number Storage Stability Organism
2.3.1.37 -15°C, 20 mM potassium phosphate buffer, pH 7.6, 1 mM dithioerythritol, 0.01 mM pyridoxal 5'-phosphate, 30% loss of activity after 2 weeks Gallus gallus
2.3.1.37 4°C, 20 mM potassium phosphate buffer, pH 7.6, 1 mM dithioerythritol, 0.01 mM pyridoxal 5'-phosphate, 20% loss of activity after 2 weeks Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.37 succinyl-CoA + glycine
-
 Gallus gallus 5-aminolevulinate + CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.37 dimer 2 * 49000, SDS-PAGE Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.37 pyridoxal 5'-phosphate required Gallus gallus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.37 0.035
-
 hemin
-
 Gallus gallus