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Literature summary extracted from

  • Borthwick, I.A.; Srivastava, G.; Pirola, B.A.; May, B.K.; Elliott, W.H.
    Purification of hepatic mitochondrial 5-aminolevulinate synthase (1986), Methods Enzymol., 123, 395-401.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.37 mitochondrial inner membrane loosely bound Gallus gallus 5743
-
2.3.1.37 mitochondrial inner membrane loosely bound Rattus norvegicus 5743
-
2.3.1.37 mitochondrial matrix
-
 Gallus gallus 5759
-
2.3.1.37 mitochondrial matrix
-
 Rattus norvegicus 5759
-
2.3.1.37 mitochondrion
-
 Gallus gallus 5739
-
2.3.1.37 mitochondrion
-
 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.37 succinyl-CoA + glycine Gallus gallus rate-limiting enzyme of heme biosynthesis 5-aminolevulinate + CoA + CO2
-
 ?
2.3.1.37 succinyl-CoA + glycine Rattus norvegicus rate-limiting enzyme of heme biosynthesis 5-aminolevulinate + CoA + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.37 Gallus gallus
-
-
-
2.3.1.37 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.37
-
 Gallus gallus
2.3.1.37 difficulties due to the tendency of the enzyme to form large MW aggregates and to its susceptibility to proteolytic degradation Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.37 embryo
-
 Gallus gallus
-
2.3.1.37 embryo
-
 Rattus norvegicus
-
2.3.1.37 liver fetal Gallus gallus
-
2.3.1.37 liver fetal Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.37 0.16
-
 purified enzyme Rattus norvegicus
2.3.1.37 0.58
-
 purified enzyme Gallus gallus

Storage Stability

EC Number Storage Stability Organism
2.3.1.37 -195°C, following an initial loss of 20% of activity due to freeze-thawing the enzyme loses no further activity for at least 6 months Gallus gallus
2.3.1.37 -195°C, following an initial loss of 20% of activity due to freeze-thawing the enzyme loses no further activity for at least 6 months Rattus norvegicus
2.3.1.37 4°C, 50 mM Tris-HCl, 100 mM glycine, 0.1 mM pyridoxal 5'-phosphate, 1 mM dithioerythritol, pH 7.6, 5 mM 5'-AMP, stable for at least 4 months Gallus gallus
2.3.1.37 4°C, 50 mM Tris-HCl, 100 mM glycine, 0.1 mM pyridoxal 5'-phosphate, 1 mM dithioerythritol, pH 7.6, 5 mM 5'-AMP, stable for at least 4 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.37 succinyl-CoA + glycine
-
 Gallus gallus 5-aminolevulinate + CoA + CO2
-
 ?
2.3.1.37 succinyl-CoA + glycine
-
 Rattus norvegicus 5-aminolevulinate + CoA + CO2
-
 ?
2.3.1.37 succinyl-CoA + glycine rate-limiting enzyme of heme biosynthesis Gallus gallus 5-aminolevulinate + CoA + CO2
-
 ?
2.3.1.37 succinyl-CoA + glycine rate-limiting enzyme of heme biosynthesis Rattus norvegicus 5-aminolevulinate + CoA + CO2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.37 37
-
 assay at Gallus gallus
2.3.1.37 37
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.37 pyridoxal 5'-phosphate
-
 Gallus gallus
2.3.1.37 pyridoxal 5'-phosphate
-
 Rattus norvegicus