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Literature summary extracted from

  • Yamagata, S.
    Partial purification and some properties of homoserine O-acetyltransferase of a methionine auxotroph of Saccharomyces cerevisiae (1987), J. Bacteriol., 169, 3458-3463.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.31 additional information methionine auxotroph strains show no activity Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.31 DL-Penicillamine 3.3 mM, complete inhibition Saccharomyces cerevisiae
2.3.1.31 L-cysteine weak Saccharomyces cerevisiae
2.3.1.31 L-homocysteine
-
 Saccharomyces cerevisiae
2.3.1.31 L-methionine no inhibition; repression of enzyme expression Saccharomyces cerevisiae
2.3.1.31 additional information no inhibition by MgCl2, MnCl2, ZnCl2, hydroxylamine, phenylhydrazine, D-homoserine, methionine, L-tyrosine, L-serine and nucleotides Saccharomyces cerevisiae
2.3.1.31 O-acetyl-L-homoserine
-
 Saccharomyces cerevisiae
2.3.1.31 O-acetyl-L-serine weak Saccharomyces cerevisiae
2.3.1.31 O-phospho-L-homoserine weak Saccharomyces cerevisiae
2.3.1.31 O-succinyl-L-homoserine weak Saccharomyces cerevisiae
2.3.1.31 S-adenosyl-L-homocysteine weak Saccharomyces cerevisiae
2.3.1.31 S-adenosyl-L-methionine weak Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.31 0.012
-
 acetyl-CoA deacylation Saccharomyces cerevisiae
2.3.1.31 0.027
-
 acetyl-CoA
-
 Saccharomyces cerevisiae
2.3.1.31 1
-
 L-homoserine
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.31 101000 104000 sucrose density gradient centrifugation, gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.31 Saccharomyces cerevisiae
-
 haploid wild-type strain (a-mating type) and met-auxotroph mutant strains 6,8,12,13,15, and 17
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.31 partial, mutant strain 6 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.31 67.4
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.31 acetyl-CoA + L-homoserine succinyl-CoA cannot replace acetyl-CoA Saccharomyces cerevisiae CoA + O-acetyl-L-homoserine
-
 ir
2.3.1.31 acetyl-CoA + L-homoserine not D-homoserine or any other hydroxy-L-amino acid Saccharomyces cerevisiae CoA + O-acetyl-L-homoserine
-
 ir
2.3.1.31 additional information deacylates acetyl-CoA in the absence of L-homoserine Saccharomyces cerevisiae ?
-
 ?
2.3.1.31 additional information catalyzes reversible L-homoserine/O-acetyl-L-homoserine exchange in the absence of acetyl-CoA Saccharomyces cerevisiae ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.31 30
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.31 additional information
-
 pI: 4.0 Saccharomyces cerevisiae
2.3.1.31 7.5
-
-
 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.31 7.8
-
 most stable Saccharomyces cerevisiae