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Literature summary extracted from

  • Wyman, A.; Paulus, H.
    Purification and properties of homoserine transacetylase from Bacillus polymyxa (1975), J. Biol. Chem., 250, 3897-3903.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.31 Ethionine
-
 Paenibacillus polymyxa
2.3.1.31 L-methionine addition of S-adenosyl-L-methionine enhances the inhibitory effect; multivalent feed-back inhibition Paenibacillus polymyxa
2.3.1.31 additional information no inhibition by Cu2+, Cd2+, Co2+, Fe2+, Hg2+, Mg2+, Mn2+, Ni2+, CoA Paenibacillus polymyxa
2.3.1.31 S-adenosyl-L-methionine addition of L-methionine enhances the inhibitory effect; multivalent feed-back inhibition Paenibacillus polymyxa
2.3.1.31 Zn2+ reversible Paenibacillus polymyxa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.31 0.2
-
 acetyl-CoA
-
 Paenibacillus polymyxa
2.3.1.31 0.2
-
 L-homoserine
-
 Paenibacillus polymyxa
2.3.1.31 10
-
 L-homoserine exchange reaction Paenibacillus polymyxa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.31 37000
-
 1 * 37000, SDS-PAGE Paenibacillus polymyxa
2.3.1.31 40000
-
 sucrose density gradient centrifugation, PAGE Paenibacillus polymyxa

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.31 Paenibacillus polymyxa
-
 met-auxotroph strain M4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.31 partial Paenibacillus polymyxa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.31 1.17
-
-
 Paenibacillus polymyxa

Storage Stability

EC Number Storage Stability Organism
2.3.1.31 -10°C, t1/2: 3 months Paenibacillus polymyxa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.31 acetyl-CoA + L-homoserine
-
 Paenibacillus polymyxa CoA + O-acetyl-L-homoserine
-
 ?
2.3.1.31 additional information catalyzes reversible L-homoserine/O-acetyl-L-homoserine exchange in the absence of acetyl-CoA Paenibacillus polymyxa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.31 monomer 1 * 37000, SDS-PAGE Paenibacillus polymyxa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.31 8
-
 assay at Paenibacillus polymyxa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.31
-
-
 t1/2: 15 min Paenibacillus polymyxa
2.3.1.31 8
-
 t1/2: 10 min Paenibacillus polymyxa
2.3.1.31 25
-
 most rapid and irreversible inactivation Paenibacillus polymyxa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.31 7.5 8
-
 Paenibacillus polymyxa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.31 6.6 9 more than half-maximal activity over this range Paenibacillus polymyxa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.31 0.003
-
 Zn2+
-
 Paenibacillus polymyxa