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Literature summary extracted from

  • Masuyoshi, S.; Okubo, T.; Inoue, M.; Mitsuhashi, S.
    Purification and some properties of a chloramphenicol acetyltransferase mediated by plasmids from Vibrio anguillarum (1988), J. Biochem., 104, 131-135.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.28 plasmid Rms418 is transferred from Vibrio anguillarum to Escherichia coli K-12 Vibrio anguillarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.28 5,5'-dithiobis(2-nitrobenzoic acid) 0.025 mM, 82% inhibition Vibrio anguillarum
2.3.1.28 EDTA 0.1 mM, 24% inhibition Vibrio anguillarum
2.3.1.28 Hg2+ 0.01 mM, complete inhibition Vibrio anguillarum
2.3.1.28 I2 0.1 mM, 20% inhibition Vibrio anguillarum
2.3.1.28 PCMB 0.1 mM, complete inhibition Vibrio anguillarum
2.3.1.28 Zn2+ 0.1 mM, 15% inhibition Vibrio anguillarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.28 0.0345
-
 chloramphenicol
-
 Vibrio anguillarum
2.3.1.28 0.156
-
 acetyl-CoA
-
 Vibrio anguillarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.28 22000
-
 3 * 22000, SDS-PAGE Vibrio anguillarum
2.3.1.28 69000
-
 gel filtration Vibrio anguillarum

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.28 Vibrio anguillarum
-
 enzyme is encoded by plasmid Rms418
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.28 plasmid Rms418 is transferred from Vibrio anguillarum to E. coli K-12 Vibrio anguillarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.28 71.4
-
-
 Vibrio anguillarum

Storage Stability

EC Number Storage Stability Organism
2.3.1.28 -80 C, 20% glycerol, stable for more than 3 months Vibrio anguillarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.28 acetyl-CoA + chloramphenicol
-
 Vibrio anguillarum CoA + chloramphenicol 3-acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.28 trimer 3 * 22000, SDS-PAGE Vibrio anguillarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.28 53
-
 0.1 mM chloramphenicol and 0.1 mM acetyl-CoA, 50 mM Tris-HCl buffer, pH 7.8, half-life: 100 min Vibrio anguillarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.28 7.8
-
-
 Vibrio anguillarum