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Literature summary extracted from

  • Becker, A.; Böttcher, A.; Lackner, K.J.; Fehringer, P.; Notka, F.; Aslanidis, C.; Schmitz, G.
    Purification, cloning, and expression of a human enzyme with acyl coenzyme A:cholesterol acyltransferase activity, which is identical to liver carboxylesterase (1994), Arterioscler. Thromb., 14, 1346-1355.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.26 expression in enzyme-deficient mutant chinese hamster ovary cells Homo sapiens
2.3.1.26 expression of an antisense cDNA in HepG2 cells Homo sapiens
2.3.1.26 sequence analysis and human cDNA clones indicate homology to liver carboxylesterase Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.26 microsome
-
Homo sapiens
-
-
2.3.1.26 microsome
-
Sus scrofa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.26 58000
-
x * 58000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.26 long-chain fatty acyl-CoA + cholesterol Sus scrofa
-
CoA + cholesteryl long-chain fatty acyl ester
-
?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol Homo sapiens the enzyme is relevant for cellular cholesterol esterification in vivo, the regulation in human mononuclear phagocytes indicates that the enzyme is also involved in foam cell formation during early atherogenesis CoA + cholesteryl long-chain fatty acyl ester
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.26 Homo sapiens
-
-
-
2.3.1.26 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.26 based on solubilization in CHAPS, preparative free-flow isotachophoresis, and further separation of the protein showing enzyme activity by preparative free-flow IEF, followed by removal of nonglycosylated proteins by ConA-affinity chromatography and preparative SDS-PAGE Sus scrofa

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.26 reconstitution with liposomes Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.26 liver
-
Homo sapiens
-
2.3.1.26 liver
-
Sus scrofa
-
2.3.1.26 macrophage
-
Homo sapiens
-
2.3.1.26 monocyte
-
Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.26 long-chain fatty acyl-CoA + cholesterol
-
Sus scrofa CoA + cholesteryl long-chain fatty acyl ester
-
?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol the enzyme is relevant for cellular cholesterol esterification in vivo, the regulation in human mononuclear phagocytes indicates that the enzyme is also involved in foam cell formation during early atherogenesis Homo sapiens CoA + cholesteryl long-chain fatty acyl ester
-
?
2.3.1.26 oleoyl-CoA + cholesterol
-
Homo sapiens CoA + cholesteryl oleate
-
?
2.3.1.26 oleoyl-CoA + cholesterol
-
Sus scrofa CoA + cholesteryl oleate
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.26 ? x * 58000, SDS-PAGE Sus scrofa