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Literature summary extracted from

  • Billheimer, J.T.
    Cholesterol acyltransferase (1985), Methods Enzymol., 111, 286-293.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.26 albumin
-
 Homo sapiens
2.3.1.26 albumin required in the assay medium to prevent the high concentration of oleoly-CoA from inhibiting the enzyme presumably by disrupting the microsomal membrane Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.26 (Z)-17(20)-dehydrocholesterol inhibits esterification of endogenous cholesterol Rattus norvegicus
2.3.1.26 20-Methylcholesterol inhibits esterification of endogenous cholesterol Rattus norvegicus
2.3.1.26 5alpha-dihydroprogesterone inhibits esterification of endogenous cholesterol Homo sapiens
2.3.1.26 acetone
-
 Rattus norvegicus
2.3.1.26 acetone about 40% inhibition at a final concentration of 1% v/v Saccharomyces cerevisiae
2.3.1.26 androsterone inhibits esterification of endogenous cholesterol Rattus norvegicus
2.3.1.26 butanol
-
 Rattus norvegicus
2.3.1.26 butanol about 40% inhibition at a final concentration of 1% v/v Saccharomyces cerevisiae
2.3.1.26 Cycloartenol inhibits esterification of exogenous cholesterol Rattus norvegicus
2.3.1.26 deoxycorticosterone inhibits esterification of endogenous cholesterol Homo sapiens
2.3.1.26 deoxycorticosterone
-
 Rattus norvegicus
2.3.1.26 Detergents
-
 Homo sapiens
2.3.1.26 Detergents above their critical micelle concentration, e.g. cholate, deoxycholate, cetyltriethylammonium bromide, Triton X-100, Tween 20, Tween 80, Nonidet P40 Rattus norvegicus
2.3.1.26 Detergents
-
 Sus scrofa
2.3.1.26 Digitonin
-
 Rattus norvegicus
2.3.1.26 ergosterol inhibits esterification of exogenous cholesterol Rattus norvegicus
2.3.1.26 ethanol
-
 Rattus norvegicus
2.3.1.26 ethanol about 40% inhibition at a final concentration of 1% v/v Saccharomyces cerevisiae
2.3.1.26 Filipin
-
 Rattus norvegicus
2.3.1.26 lanosterol inhibits esterification of exogenous cholesterol Helicoverpa zea
2.3.1.26 lanosterol inhibits esterification of exogenous cholesterol Rattus norvegicus
2.3.1.26 oleoyl-CoA inhibits at high concentrations, the inhibition is prevented by serum albumin Rattus norvegicus
2.3.1.26 organic solvents inhibit by perturbing the membrane structure Rattus norvegicus
2.3.1.26 Pregn-5-en-3beta-ol inhibits esterification of endogenous cholesterol Rattus norvegicus
2.3.1.26 pregnenolone inhibits esterification of endogenous cholesterol Homo sapiens
2.3.1.26 progesterone inhibits esterification of endogenous cholesterol reversibly Homo sapiens
2.3.1.26 progesterone inhibits esterification of endogenous cholesterol reversibly Rattus norvegicus
2.3.1.26 SC-31769 20-oxa analog of 7-ketosterol Homo sapiens
2.3.1.26 SC-31769 20-oxa analog of 7-ketosterol Rattus norvegicus
2.3.1.26 sitosterol inhibits esterification of exogenous cholesterol Rattus norvegicus
2.3.1.26 stanolone inhibits esterification of endogenous cholesterol Rattus norvegicus
2.3.1.26 stigmasterol inhibits esterification of exogenous cholesterol Rattus norvegicus
2.3.1.26 testosterone inhibits esterification of endogenous cholesterol Homo sapiens
2.3.1.26 testosterone
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.26 additional information
-
 additional information
-
 Homo sapiens
2.3.1.26 additional information
-
 additional information use of kinetic parameters such as Km is of limited value due to the physical properties of oleoyl-CoA in solution and the addition of serum albumin to the assay Rattus norvegicus
2.3.1.26 0.055
-
 oleoyl-CoA
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.26 endoplasmic reticulum
-
 Homo sapiens 5783
-
2.3.1.26 endoplasmic reticulum
-
 Rattus norvegicus 5783
-
2.3.1.26 microsome
-
 Homo sapiens
-
-
2.3.1.26 microsome
-
 Rattus norvegicus
-
-
2.3.1.26 microsome
-
 Sus scrofa
-
-
2.3.1.26 microsome
-
 Saccharomyces cerevisiae
-
-

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.3.1.26 additional information the use of organic solvents or strong detergents for the suspension of cholesterol must be avoided because of their affect on the enzyme Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.26 Helicoverpa zea
-
-
-
2.3.1.26 Homo sapiens
-
-
-
2.3.1.26 Rattus norvegicus
-
-
-
2.3.1.26 Saccharomyces cerevisiae
-
-
-
2.3.1.26 Sus scrofa
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.26 the enzyme must be reconstituted into an artificial membrane for optimal activity Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.26 liver
-
 Homo sapiens
-
2.3.1.26 liver
-
 Rattus norvegicus
-
2.3.1.26 liver
-
 Sus scrofa
-
2.3.1.26 peritoneal macrophage
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.26 additional information
-
-
 Saccharomyces cerevisiae
2.3.1.26 additional information
-
 assay method Homo sapiens
2.3.1.26 additional information
-
 assay method Rattus norvegicus
2.3.1.26 additional information
-
 assay method Sus scrofa
2.3.1.26 additional information
-
 assay method Helicoverpa zea

Storage Stability

EC Number Storage Stability Organism
2.3.1.26 -80°C, stable for several weeks Rattus norvegicus
2.3.1.26 -80°C, stable for several weeks Saccharomyces cerevisiae
2.3.1.26 -80°C, stable for several weeks Helicoverpa zea
2.3.1.26 ovary, labile to freezing Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.26 acyl-CoA + cholesterol
-
 Homo sapiens CoA + cholesterol ester
-
 ?
2.3.1.26 acyl-CoA + cholesterol
-
 Rattus norvegicus CoA + cholesterol ester
-
 ?
2.3.1.26 acyl-CoA + cholesterol
-
 Sus scrofa CoA + cholesterol ester
-
 ?
2.3.1.26 acyl-CoA + cholesterol
-
 Saccharomyces cerevisiae CoA + cholesterol ester
-
 ?
2.3.1.26 acyl-CoA + cholesterol
-
 Helicoverpa zea CoA + cholesterol ester
-
 ?
2.3.1.26 linoleoyl-CoA + cholesterol
-
 Rattus norvegicus CoA + cholesteryl linoleate
-
 ?
2.3.1.26 additional information
-
 Homo sapiens ?
-
 ?
2.3.1.26 additional information
-
 Saccharomyces cerevisiae ?
-
 ?
2.3.1.26 additional information a part of the substrate selectivity may be due to the presence of acyl-CoA hydrolase in the microsomal preparations. For this enzyme, the preferred substrate is palmitoyl-CoA which is hydrolysed about 8times faster than oleoyl-CoA Rattus norvegicus ?
-
 ?
2.3.1.26 additional information investigations into the sterol specificity of the enzyme Rattus norvegicus ?
-
 ?
2.3.1.26 oleoyl-CoA + cholesterol
-
 Homo sapiens CoA + cholesteryl oleate
-
 ?
2.3.1.26 oleoyl-CoA + cholesterol
-
 Sus scrofa CoA + cholesteryl oleate
-
 ?
2.3.1.26 oleoyl-CoA + cholesterol highest activity Rattus norvegicus CoA + cholesteryl oleate
-
 ?
2.3.1.26 oleoyl-CoA + cholesterol highest activity Saccharomyces cerevisiae CoA + cholesteryl oleate
-
 ?
2.3.1.26 oleoyl-CoA + ergosterol
-
 Homo sapiens CoA + ergosteryl oleate
-
 ?
2.3.1.26 oleoyl-CoA + ergosterol
-
 Saccharomyces cerevisiae CoA + ergosteryl oleate
-
 ?
2.3.1.26 palmitoyl-CoA + cholesterol
-
 Rattus norvegicus CoA + cholesteryl palmitate
-
 ?
2.3.1.26 palmitoyl-CoA + cholesterol
-
 Saccharomyces cerevisiae CoA + cholesteryl palmitate
-
 ?
2.3.1.26 stearoyl-CoA + cholesterol
-
 Rattus norvegicus CoA + cholesteryl stearate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.26 37
-
 assay at Homo sapiens
2.3.1.26 37
-
 assay at Rattus norvegicus
2.3.1.26 37
-
 assay at Sus scrofa
2.3.1.26 37
-
 assay at Helicoverpa zea