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Literature summary extracted from

  • Deka, N.; Sun, G.Y.; MacQuarrie, R.
    Purification and properties of acyl-CoA:1-acyl-sn-glycero-3-phosphocholine-O-acyltransferase from bovine brain microsomes (1986), Arch. Biochem. Biophys., 246, 554-563.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.23 Arachidonoyl-CoA high concentration Bos taurus
2.3.1.23 Trypsin inclusion in liposomes protects Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.23 additional information
-
 additional information kinetic data of soluble and liposome-bound enzyme Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.23 microsome
-
 Bos taurus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.23 43000
-
 x * 43000, brain enzyme, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.23 acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine Bos taurus participates in deacylation/reacylation cycle of cell membranes CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.23 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.23 solubilized with lysolecithin, reactive Blue-2-agarose, DEAE cellulose, Matrex Green A Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.23 brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.23 0.079
-
 acyl donor oleoyl-CoA Bos taurus
2.3.1.23 0.134
-
 acyl donor linoleoyl-CoA Bos taurus
2.3.1.23 0.61
-
 acyl donor arachidonoyl-CoA Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.3.1.23 -20°C, liposome-bound enzyme, at least 10 h Bos taurus
2.3.1.23 -20°C, soluble enzyme, less than 10 h Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.23 acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
 Bos taurus CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
 r
2.3.1.23 acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine participates in deacylation/reacylation cycle of cell membranes Bos taurus CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
 ?
2.3.1.23 acyl-CoA + 1-palmitoyl-sn-glycero-3-phosphocholine no activity with 1-myristoyl-sn-glycero-3-phosphocholine Bos taurus CoA + 1-palmitoyl-2-acyl-sn-glycero-3-phosphocholine
-
 r
2.3.1.23 acyl-CoA + 1-stearoyl-sn-glycero-3-phosphocholine no activity with 1-myristoyl-sn-glycero-3-phosphocholine Bos taurus CoA + 1-stearoyl-2-acyl-sn-glycero-3-phosphocholine
-
 r
2.3.1.23 arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine preferred acyl donor Bos taurus CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphocholine
-
 r
2.3.1.23 cis-11-eicosaenoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine 13% of activity with arachidonoyl-CoA Bos taurus CoA + 1-acyl-2-cis-11-eicosaenoyl-sn-glycero-3-phosphocholine
-
 r
2.3.1.23 decanoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine 58% of activity with arachidonoyl-CoA Bos taurus CoA + 1-acyl-2-decanoyl-sn-glycero-3-phosphocholine
-
 r
2.3.1.23 eicosatrienoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine 20% of activity with arachidonoyl-CoA Bos taurus CoA + 1-acyl-2-eicosatrienoyl-sn-glycero-3-phosphocholine
-
 r
2.3.1.23 linolenoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine 8% of activity with arachidonoyl-CoA Bos taurus CoA + 1-acyl-2-linolenoyl-sn-glycero-3-phosphocholine
-
 r
2.3.1.23 linoleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
 Bos taurus CoA + 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine
-
 r
2.3.1.23 myristoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine 41% of activity with arachidonoyl-CoA Bos taurus CoA + 1-acyl-2-myristoyl-sn-glycero-3-phosphocholine
-
 r
2.3.1.23 oleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
 Bos taurus CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.23 ? x * 43000, brain enzyme, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.23 37
-
-
 Bos taurus