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Literature summary extracted from

  • Nicot, C.; Relat, J.; Woldegiorgis, G.; Haro, D.; Marrero, P.F.
    Pig liver carnitine palmitoyltransferase: chimera studies show that both the N- and C-terminal regions of the enzyme are important for the unusual high malonyl-CoA sensitivity (2002), J. Biol. Chem., 277, 10044-10049.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.21 construction of chimeric L-CPT I with deletions and exchanged C-terminal sequences between rat and pig enzymes, expression in Pichia pastoris Rattus norvegicus
2.3.1.21 construction of chimeric L-CPT I with deletions and exchanged C-terminal sequences between rat and pig enzymes, expression in Pichia pastoris Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.21 malonyl-CoA sensitivity to inhibition of deletion and chimeric L-CPT I mutants Rattus norvegicus
2.3.1.21 malonyl-CoA sensitivity to inhibition of deletion and chimeric L-CPT I mutants Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.21 additional information
-
additional information Km values of deletion and chimeric L-CPT I mutants Rattus norvegicus
2.3.1.21 additional information
-
additional information Km values of deletion and chimeric L-CPT I mutants Sus scrofa
2.3.1.21 0.035
-
palmitoyl-CoA recombinant wild-type L-CPT I Sus scrofa
2.3.1.21 0.043
-
palmitoyl-CoA recombinant wild-type L-CPT I Rattus norvegicus
2.3.1.21 0.1
-
L-carnitine recombinant wild-type L-CPT I Rattus norvegicus
2.3.1.21 0.126
-
L-carnitine recombinant wild-type L-CPT I Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.21 acyl-CoA + L-carnitine Rattus norvegicus
-
CoA + L-acylcarnitine
-
?
2.3.1.21 acyl-CoA + L-carnitine Sus scrofa
-
CoA + L-acylcarnitine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.21 Rattus norvegicus
-
-
-
2.3.1.21 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.21 additional information
-
activities of deletion and chimeric L-CPT I mutants Rattus norvegicus
2.3.1.21 additional information
-
activities of deletion and chimeric L-CPT I mutants Sus scrofa
2.3.1.21 0.0052
-
recombinant wild-type L-CPT I Sus scrofa
2.3.1.21 0.0078
-
recombinant wild-type L-CPT I Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.21 acyl-CoA + L-carnitine
-
Rattus norvegicus CoA + L-acylcarnitine
-
?
2.3.1.21 acyl-CoA + L-carnitine
-
Sus scrofa CoA + L-acylcarnitine
-
?
2.3.1.21 palmitoyl-CoA + L-carnitine chimeric L-CPT I with exchanged C-terminals between pig and rat enzymes Rattus norvegicus CoA + L-palmitoylcarnitine
-
?
2.3.1.21 palmitoyl-CoA + L-carnitine chimeric L-CPT I with exchanged C-terminals between pig and rat enzymes Sus scrofa CoA + L-palmitoylcarnitine
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.21 30
-
assay at Rattus norvegicus
2.3.1.21 30
-
assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.21 7
-
assay at Rattus norvegicus
2.3.1.21 7
-
assay at Sus scrofa