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Literature summary extracted from

  • Husaka, K.; Schiele, U.; Numa, S.
    Diacylglycerol acyltransferase from rat liver microsomes. Separation and acyl-donor specificity (1977), Eur. J. Biochem., 76, 113-118.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.20 DTT activation, microsomal preparation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.20 5,5'-dithio-bis(2-nitrobenzoic acid) 11% inhibition at 1 mM Rattus norvegicus
2.3.1.20 iodoacetamide 15% inhibition at 1 mM, DTT protects Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 microsome
-
 Rattus norvegicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.20 partial, with 1-acylglycerolphosphorylcholine acyltransferase Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacylglycerol
-
 Rattus norvegicus CoA + triacylglycerol
-
 ?
2.3.1.20 arachidonoyl-CoA + 1,2-diacylglycerol
-
 Rattus norvegicus CoA + 3-arachidonoyl-1,2-diacylglycerol
-
 ?
2.3.1.20 linoleoyl-CoA + 1,2-diacylglycerol
-
 Rattus norvegicus CoA + 1,2-diacyl-3-linoleoylglycerol
-
 ?
2.3.1.20 myristoyl-CoA + 1,2-diacylglycerol
-
 Rattus norvegicus CoA + 1,2-diacyl-3-myristoylglycerol
-
 ?
2.3.1.20 oleoyl-CoA + 1,2-diacylglycerol
-
 Rattus norvegicus CoA + 1,2-diacyl-3-oleoylglycerol
-
 ?
2.3.1.20 palmitoyl-CoA + 1,2-diacylglycerol
-
 Rattus norvegicus CoA + 1,2-diacyl-3-palmitoylglycerol
-
 ?
2.3.1.20 stearoyl-CoA + 1,2-diacylglycerol weak donor Rattus norvegicus CoA + 1,2-diacyl-3-stearoylglycerol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.20 additional information
-
 assay at various temperatures from 24°C to 40°C Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.20 37
-
 t1/2: 8 min Rattus norvegicus