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Literature summary extracted from

  • Weselake, R.J.; Taylor, D.C.; Pomeroy, D.C.; Lawson, S.L.; Underhill, E.W.
    Properties of diacylglycerol acyltransferase from microspore-derived embryos of Brassica napus (1991), Phytochemistry, 30, 3533-3538.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.20 2-Bromooctanoate 50% inhibition at 1.5 mM Brassica napus
2.3.1.20 cetyltrimethylammonium bromide
-
 Brassica napus
2.3.1.20 CHAPS
-
 Brassica napus
2.3.1.20 deoxycholate
-
 Brassica napus
2.3.1.20 n-octyl-beta-D-glucopyranoside weak Brassica napus
2.3.1.20 sodiumdodecyl sulfate
-
 Brassica napus
2.3.1.20 Triton X-100 microsomal preparation Brassica napus
2.3.1.20 Tween 20
-
 Brassica napus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 microsome
-
 Brassica napus
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Brassica napus
-
 cv. Reston
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 embryo microspore-derived Brassica napus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacylglycerol
-
 Brassica napus CoA + triacylglycerol
-
 ?
2.3.1.20 erucoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Brassica napus CoA + 3-erucoyl-1,2-dioleoyl-sn-glycerol
-
 ?
2.3.1.20 oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Brassica napus CoA + trioleoylglycerol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.20 30 40
-
 Brassica napus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.20 20 50 about 70% of maximal activity at 20°C, about half-maximal activity at 50°C Brassica napus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.20 40
-
 stable below Brassica napus
2.3.1.20 45
-
 t1/2: 10 min Brassica napus