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Literature summary extracted from

  • Polokoff, M.A.; Bell, R.M.
    Solubilization, partial purification and characterization of rat liver microsomal diacylglycerol acyltransferase (1980), Biochim. Biophys. Acta, 618, 129-142.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.20 phosphocholine from soy bean, activation Rattus norvegicus
2.3.1.20 Phospholipid microsomal Rattus norvegicus

General Stability

EC Number General Stability Organism
2.3.1.20 MgCl2 accelerates loss of activity during storage at 4°C, dilution increases stability, cholate-solubilized activity is more stable than deoxycholate-solubilized activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.20 cholate not up to 4 mM Rattus norvegicus
2.3.1.20 palmitoyl-CoA high concentration Rattus norvegicus
2.3.1.20 Triton X-100 above 0.05 mM Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.20 Mg2+ strongly dependent Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.20 partial Rattus norvegicus
2.3.1.20 with Triton X-100, deoxycholate or cholate Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.20 0.0024 0.038
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.3.1.20 -80°C, at least 24 h, deoxycholate solubilized enzyme Rattus norvegicus
2.3.1.20 -80°C, undiluted, several weeks, cholate solubilized enzyme Rattus norvegicus
2.3.1.20 4°C, 20-30% loss of activity after 24 h, cholate solubilized enzyme, dilution restores activity Rattus norvegicus
2.3.1.20 4°C, about 65-85% loss of activity after 24 h, deoxycholate solubilized enzyme, partially restorable by dialysis against buffer lacking detergent Rattus norvegicus
2.3.1.20 4°C, inactivation after 24 h in the presence of 8 mM MgCl2, deoxycholate solubilized enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Rattus norvegicus CoA + 1,2-dioleoyl-3-palmitoylglycerol
-
 ?