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Literature summary extracted from

  • Lewendon, A.; Ellis, J.; Shaw, W.V.
    Structural and mechanistic studies of galactoside acetyltransferase, the Escherichia coli LacA gene product (1995), J. Biol. Chem., 270, 26326-26331.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.18 expressed in Escherichia coli Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.18 H115A kcat highly decreased, histidyl residue has an important catalytic role Escherichia coli
2.3.1.18 S162A kcat increased, Ser162 not catalytically important Escherichia coli
2.3.1.18 W139F kcat 10fold decreased Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.18 iodoacetamide complete inactivation Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.18 0.059
-
 acetyl-CoA phenyl-beta-D-galactopyranoside Escherichia coli
2.3.1.18 0.065
-
 acetyl-CoA p-nitrophenyl-beta-D-galactopyranoside Escherichia coli
2.3.1.18 0.082
-
 acetyl-CoA o-nitrophenyl-beta-D-galactopyranoside Escherichia coli
2.3.1.18 0.098
-
 propionyl-CoA p-nitrophenyl-beta-D-galactopyranoside Escherichia coli
2.3.1.18 0.104
-
 acetyl-CoA p-nitrophenyl-beta-D-galactopyranoside Escherichia coli
2.3.1.18 0.221
-
 butyryl-CoA p-nitrophenyl-beta-D-galactopyranoside Escherichia coli
2.3.1.18 0.266
-
 acetyl-CoA p-nitrophenyl-beta-D-lactopyranoside Escherichia coli
2.3.1.18 25.2
-
 p-nitrophenyl-beta-D-galactopyranoside acetyl donor: butyrylCoA Escherichia coli
2.3.1.18 40.2
-
 p-nitrophenyl-beta-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli
2.3.1.18 45.6
-
 p-nitrophenyl-beta-D-galactopyranoside acetyl donor: propionyl-CoA Escherichia coli
2.3.1.18 57.9
-
 p-nitrophenyl-alpha-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli
2.3.1.18 60.2
-
 p-nitrophenyl-beta-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli
2.3.1.18 63.4
-
 p-nitrophenyl-beta-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli
2.3.1.18 63.4
-
 phenyl-beta-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli
2.3.1.18 67.9
-
 o-nitrophenyl-beta-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.18 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.18 wild-type and mutant enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.18 4-nitrophenyl alpha-D-galactopyranoside + acetyl-CoA
-
 Escherichia coli 4-nitrophenyl 6-O-acetyl-alpha-D-galactopyranoside + CoA
-
 ?
2.3.1.18 acetyl-CoA + beta-D-phenylgalactoside
-
 Escherichia coli CoA + 6-O-acetyl-beta-D-phenylgalactoside
-
 ?
2.3.1.18 acetyl-CoA + o-nitrophenyl-beta-D-galactopyranoside
-
 Escherichia coli CoA + o-nitrophenyl 6-O-acetyl-beta-D-galactoside
-
 ?
2.3.1.18 acetyl-CoA + p-nitrophenyl-beta-D-galactopyranoside
-
 Escherichia coli CoA + p-nitrophenyl 6-O-acetyl-beta-D-galactopyranoside
-
 ?
2.3.1.18 acetyl-CoA + p-nitrophenyl-beta-D-glucopyranoside
-
 Escherichia coli CoA + p-nitrophenyl 6-O-acetyl-beta-D-glucopyranoside
-
 ?
2.3.1.18 acetyl-CoA + p-nitrophenyl-beta-D-lactopyranoside
-
 Escherichia coli CoA + p-nitrophenyl 6-O-acetyl-beta-D-lactopyranoside
-
 ?
2.3.1.18 butyryl-CoA + p-nitrophenyl-beta-D-galactopyranoside
-
 Escherichia coli CoA + p-nitrophenyl 6-O-butyryl-beta-D-galactopyranoside
-
 ?
2.3.1.18 additional information enzyme catalyses transfer of an acetyl group from acetyl-CoA to the 6-hydroxyl group of a beta-D-galactoside Escherichia coli ?
-
 ?
2.3.1.18 propionyl-CoA + p-nitrophenyl-beta-D-galactopyranoside
-
 Escherichia coli CoA + p-nitrophenyl 6-O-propionyl-beta-D-galactopyranoside
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.18 0.054
-
 p-nitrophenyl-beta-D-galactopyranoside acetyl donor: butyryl-CoA Escherichia coli
2.3.1.18 1.42
-
 4-nitrophenyl alpha-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli
2.3.1.18 1.84
-
 p-nitrophenyl-beta-D-glucopyranoside acetyl donor: acetyl-CoA Escherichia coli
2.3.1.18 1.84
-
 p-nitrophenyl-beta-D-lactopyranoside acetyl donor: acetyl-CoA Escherichia coli
2.3.1.18 5.8
-
 p-nitrophenyl-beta-D-galactopyranoside acetyl donor: propionyl-CoA Escherichia coli
2.3.1.18 8.9
-
 o-nitrophenyl-beta-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli
2.3.1.18 13
-
 phenyl-beta-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli
2.3.1.18 14.7
-
 p-nitrophenyl-beta-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli