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Literature summary extracted from

  • Goldstein, F.B.
    Biosynthesis of N-acetyl-L-aspartic acid (1959), J. Biol. Chem., 234, 2702-2706.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.17 Acyl-AMP derivatives e.g. acetyl-AMP, butyryl-AMP, strong Rattus norvegicus
2.3.1.17 benzoyl-CoA weak Rattus norvegicus
2.3.1.17 fluoroacetyl-CoA weak Rattus norvegicus
2.3.1.17 propionyl-CoA weak Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.17 additional information
-
 additional information Km value for acetyl-CoA Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.17 KBr stimulates Rattus norvegicus
2.3.1.17 KCl 2fold enzyme activity at 0.05 - 0.1 M Rattus norvegicus
2.3.1.17 KClO3 stimulates Rattus norvegicus
2.3.1.17 KCNS stimulates Rattus norvegicus
2.3.1.17 KI stimulates Rattus norvegicus
2.3.1.17 KNO3 stimulates Rattus norvegicus
2.3.1.17 LiCl stimulates Rattus norvegicus
2.3.1.17 NaCl stimulates Rattus norvegicus
2.3.1.17 NaNO2 stimulates Rattus norvegicus
2.3.1.17 NH4Cl stimulates Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.17 acetyl-CoA + L-aspartate Rattus norvegicus
-
 CoA + N-acetyl-L-aspartic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.17 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.17 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.17 brain
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.17 0-5°C, 8-10 mg protein/ml, stable for weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.17 acetyl-CoA + L-aspartate
-
 Rattus norvegicus CoA + N-acetyl-L-aspartic acid
-
 ?
2.3.1.17 acetyl-CoA + L-aspartate highly specific Rattus norvegicus CoA + N-acetyl-L-aspartic acid
-
 ?
2.3.1.17 additional information reduced CoA with acetyl-AMP can substitute for acetyl-CoA, about 50% of activity with acetyl-CoA Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.17 37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.17 100
-
 no enzyme activity after boiling Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.17 6 6.8
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.17 5.2 8 about 13% of maximal activity at pH 5.2, about 50% of maximal activity at pH 8.0 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.17 additional information
-
 additional information Ki values for various inhibitors Rattus norvegicus