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Literature summary extracted from

  • Walker, K.; Ketchum, R.E.; Hezari, M.; Gatfield, D.; Goleniowski, M.; Barthol, A.; Croteau, R.
    Partial purification and characterization of acetyl coenzyme A: taxa-4(20),11(12)-dien-5alpha-ol O-acetyl transferase that catalyzes the first acylation step of taxol biosynthesis (1999), Arch. Biochem. Biophys., 364, 273-279.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.162 medicine understanding of biosynthetic pathway of taxol, target: possibly biological production of taxol, taxol or paclitaxel is now well established as a potent chemotherapeutic drug Taxus cuspidata
2.3.1.162 medicine understanding of biosynthetic pathway of taxol, target: possibly biological production of taxol, taxol or paclitaxel is now well established as a potent chemotherapeutic drug Taxus canadensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.162 CoA weak Taxus canadensis
2.3.1.162 CoA weak Taxus cuspidata
2.3.1.162 N-ethylmaleimide weak Taxus canadensis
2.3.1.162 N-ethylmaleimide weak Taxus cuspidata
2.3.1.162 p-hydroxymercuribenzoate weak Taxus canadensis
2.3.1.162 p-hydroxymercuribenzoate weak Taxus cuspidata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.162 0.0042
-
 taxa-4(20),11-dien-5alpha-ol
-
 Taxus cuspidata
2.3.1.162 0.0042
-
 taxa-4(20),11-dien-5alpha-ol
-
 Taxus canadensis
2.3.1.162 0.0055
-
 acetyl-CoA
-
 Taxus cuspidata
2.3.1.162 0.0055
-
 acetyl-CoA
-
 Taxus canadensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.162 additional information monovalent and divalent cations show no effect on the enzyme Taxus cuspidata
2.3.1.162 additional information monovalent and divalent cations show no effect on the enzyme Taxus canadensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.162 50000
-
 gel chromatography Taxus cuspidata
2.3.1.162 50000
-
 gel chromatography Taxus canadensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.162 additional information Taxus cuspidata catalyzes the first acylation step of taxol biosynthesis ?
-
 ?
2.3.1.162 additional information Taxus canadensis catalyzes the first acylation step of taxol biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.162 Taxus canadensis
-
 methyl jasmonate-induced cells
-
2.3.1.162 Taxus cuspidata
-
 methyl jasmonate-induced cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.162 partially Taxus cuspidata
2.3.1.162 partially Taxus canadensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.162 acetyl-CoA + farnesol
-
 Taxus cuspidata CoA + ?
-
 ?
2.3.1.162 acetyl-CoA + geranylgeraniol
-
 Taxus cuspidata CoA + ?
-
 ?
2.3.1.162 acetyl-CoA + phytol
-
 Taxus cuspidata CoA + ?
-
 ?
2.3.1.162 acetyl-CoA + taxa-4(20),11(12)-dien-5alpha-ol
-
 Taxus cuspidata CoA + taxa-4(20),11-dien-5alpha-yl acetate
-
 ?
2.3.1.162 acetyl-CoA + taxa-4(20),11(12)-dien-5alpha-ol
-
 Taxus canadensis CoA + taxa-4(20),11-dien-5alpha-yl acetate
-
 ?
2.3.1.162 acetyl-CoA + taxadienol
-
 Taxus cuspidata CoA + ?
-
 ?
2.3.1.162 additional information catalyzes the first acylation step of taxol biosynthesis Taxus cuspidata ?
-
 ?
2.3.1.162 additional information catalyzes the first acylation step of taxol biosynthesis Taxus canadensis ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.162 9
-
 pI: 4.7 Taxus cuspidata
2.3.1.162 9
-
 pI: 4.7 Taxus canadensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.162 7.5 10 50% of maximal activity at pH 7.50 and pH 10.0, enzyme inactive at pH 11.0 Taxus cuspidata
2.3.1.162 7.5 10 50% of maximal activity at pH 7.50 and pH 10.0, enzyme inactive at pH 11.0 Taxus canadensis