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Literature summary extracted from

  • Springob, K.; Lukacin, R.; Ernwein, C.; Groning, I.; Matern, U.
    Specificities of functionally expressed chalcone and acridone synthases from Ruta graveolens (2000), Eur. J. Biochem., 267, 6552-6559.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.159 expression in Escherichia coli strain BL21 (DE3)pLys S and in lambda phages via Escherichia coli host, DNA sequence comparison of 4 clones and also with the DNA sequence of Ruta graveolens chalcone synthase Ruta graveolens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.159 malonyl-CoA + N-methylanthraniloyl-CoA Ruta graveolens
-
 CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.159 Ruta graveolens
-
 2 isozymes I and II
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.159 3 malonyl-CoA + N-methylanthraniloyl-CoA = 4 CoA + 1,3-dihydroxy-N-methylacridone + 3 CO2 + H2O two-step mechanism Ruta graveolens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.159 cell suspension culture
-
 Ruta graveolens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.159 additional information
-
-
 Ruta graveolens
2.3.1.159 9.54
-
 purified recombinant isozyme I Ruta graveolens
2.3.1.159 14.16
-
 purified recombinant isozyme II Ruta graveolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.159 malonyl-CoA + 4-coumaroyl-CoA isozyme I shows 16% activity and isozyme II shows 12% activity compared to the activity with N-methylanthraniloyl-CoA as substrate, respectively Ruta graveolens CoA + naringenin chalcone
-
 ?
2.3.1.159 malonyl-CoA + N-methylanthraniloyl-CoA
-
 Ruta graveolens CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.3.1.159 ACS
-
 Ruta graveolens