BRENDA - Enzyme Database

Purification and some properties of malonate decarboxylase from Pseudomonas ovalis: an oligomeric enzyme with bifunctional properties

Takamura, Y.; Kitayama, Y.; Biochem. Int. 3, 483-491 (1981)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.9
additional information
-
additional information
-
Pseudomonas putida
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.3.3
30000
-
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE
Pseudomonas putida
2.8.3.3
40000
-
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE
Pseudomonas putida
2.8.3.3
70000
-
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE
Pseudomonas putida
2.8.3.3
170000
-
gel filtration
Pseudomonas putida
4.1.1.9
30000
-
x * 30000 + x * 40000 + x * 70000, SDS-PAGE
Pseudomonas putida
4.1.1.9
40000
-
x * 30000 + x * 40000 + x * 70000, SDS-PAGE
Pseudomonas putida
4.1.1.9
70000
-
x * 30000 + x * 40000 + x * 70000, SDS-PAGE
Pseudomonas putida
4.1.1.9
170000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.3.3
acetyl-CoA + malonate
Pseudomonas putida
inducible enzyme
acetate + malonyl-CoA
-
-
?
2.8.3.3
acetyl-CoA + malonate
Pseudomonas putida IAM 1177
inducible enzyme
acetate + malonyl-CoA
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.3.3
Pseudomonas putida
-
IAM 1177
-
2.8.3.3
Pseudomonas putida IAM 1177
-
IAM 1177
-
4.1.1.9
Pseudomonas putida
-
bifunctional enzyme
-
Purification (Commentary)
EC Number
Commentary
Organism
2.8.3.3
partial
Pseudomonas putida
4.1.1.9
-
Pseudomonas putida
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.3.3
acetyl-CoA + malonate
absolutely dependent on acetyl-CoA
4864
Pseudomonas putida
acetate + malonyl-CoA
-
4864
Pseudomonas putida
?
2.8.3.3
acetyl-CoA + malonate
inducible enzyme
4864
Pseudomonas putida
acetate + malonyl-CoA
-
-
-
?
2.8.3.3
acetyl-CoA + malonate
absolutely dependent on acetyl-CoA
4864
Pseudomonas putida IAM 1177
acetate + malonyl-CoA
-
4864
Pseudomonas putida IAM 1177
?
2.8.3.3
acetyl-CoA + malonate
inducible enzyme
4864
Pseudomonas putida IAM 1177
acetate + malonyl-CoA
-
-
-
?
2.8.3.3
butyryl-CoA + malonate
-
4864
Pseudomonas putida
malonyl-CoA + butanoate
-
-
-
?
2.8.3.3
malonyl-CoA + malonate
exchange reaction
4864
Pseudomonas putida
malonyl-CoA + malonate
-
-
-
?
2.8.3.3
methylmalonyl-CoA + malonate
-
4864
Pseudomonas putida
malonyl-CoA + methylmalonate
-
-
-
?
2.8.3.3
additional information
succinyl-CoA or palmityl-CoA cannot replace acetyl-CoA
4864
Pseudomonas putida
?
-
-
-
-
2.8.3.3
additional information
succinyl-CoA or palmityl-CoA cannot replace acetyl-CoA
4864
Pseudomonas putida IAM 1177
?
-
-
-
-
2.8.3.3
propionyl-CoA + malonate
-
4864
Pseudomonas putida
malonyl-CoA + propanoate
-
-
-
?
4.1.1.9
Malonyl-CoA
-
4864
Pseudomonas putida
Acetyl-CoA + CO2
-
-
-
-
4.1.1.9
Malonyl-CoA
-
4864
Pseudomonas putida IAM 1177
Acetyl-CoA + CO2
-
-
-
-
4.1.1.9
methylmalonyl-CoA
-
4864
Pseudomonas putida
propionyl-CoA + CO2
-
-
-
-
4.1.1.9
methylmalonyl-CoA
-
4864
Pseudomonas putida IAM 1177
propionyl-CoA + CO2
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.8.3.3
tetramer
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE
Pseudomonas putida
4.1.1.9
?
x * 30000 + x * 40000 + x * 70000, SDS-PAGE
Pseudomonas putida
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.8.3.3
30
-
assay at
Pseudomonas putida
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.9
additional information
-
additional information
-
Pseudomonas putida
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.3.3
30000
-
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE
Pseudomonas putida
2.8.3.3
40000
-
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE
Pseudomonas putida
2.8.3.3
70000
-
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE
Pseudomonas putida
2.8.3.3
170000
-
gel filtration
Pseudomonas putida
4.1.1.9
30000
-
x * 30000 + x * 40000 + x * 70000, SDS-PAGE
Pseudomonas putida
4.1.1.9
40000
-
x * 30000 + x * 40000 + x * 70000, SDS-PAGE
Pseudomonas putida
4.1.1.9
70000
-
x * 30000 + x * 40000 + x * 70000, SDS-PAGE
Pseudomonas putida
4.1.1.9
170000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.3.3
acetyl-CoA + malonate
Pseudomonas putida
inducible enzyme
acetate + malonyl-CoA
-
-
?
2.8.3.3
acetyl-CoA + malonate
Pseudomonas putida IAM 1177
inducible enzyme
acetate + malonyl-CoA
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.3.3
partial
Pseudomonas putida
4.1.1.9
-
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.3.3
acetyl-CoA + malonate
absolutely dependent on acetyl-CoA
4864
Pseudomonas putida
acetate + malonyl-CoA
-
4864
Pseudomonas putida
?
2.8.3.3
acetyl-CoA + malonate
inducible enzyme
4864
Pseudomonas putida
acetate + malonyl-CoA
-
-
-
?
2.8.3.3
acetyl-CoA + malonate
absolutely dependent on acetyl-CoA
4864
Pseudomonas putida IAM 1177
acetate + malonyl-CoA
-
4864
Pseudomonas putida IAM 1177
?
2.8.3.3
acetyl-CoA + malonate
inducible enzyme
4864
Pseudomonas putida IAM 1177
acetate + malonyl-CoA
-
-
-
?
2.8.3.3
butyryl-CoA + malonate
-
4864
Pseudomonas putida
malonyl-CoA + butanoate
-
-
-
?
2.8.3.3
malonyl-CoA + malonate
exchange reaction
4864
Pseudomonas putida
malonyl-CoA + malonate
-
-
-
?
2.8.3.3
methylmalonyl-CoA + malonate
-
4864
Pseudomonas putida
malonyl-CoA + methylmalonate
-
-
-
?
2.8.3.3
additional information
succinyl-CoA or palmityl-CoA cannot replace acetyl-CoA
4864
Pseudomonas putida
?
-
-
-
-
2.8.3.3
additional information
succinyl-CoA or palmityl-CoA cannot replace acetyl-CoA
4864
Pseudomonas putida IAM 1177
?
-
-
-
-
2.8.3.3
propionyl-CoA + malonate
-
4864
Pseudomonas putida
malonyl-CoA + propanoate
-
-
-
?
4.1.1.9
Malonyl-CoA
-
4864
Pseudomonas putida
Acetyl-CoA + CO2
-
-
-
-
4.1.1.9
Malonyl-CoA
-
4864
Pseudomonas putida IAM 1177
Acetyl-CoA + CO2
-
-
-
-
4.1.1.9
methylmalonyl-CoA
-
4864
Pseudomonas putida
propionyl-CoA + CO2
-
-
-
-
4.1.1.9
methylmalonyl-CoA
-
4864
Pseudomonas putida IAM 1177
propionyl-CoA + CO2
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.8.3.3
tetramer
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE
Pseudomonas putida
4.1.1.9
?
x * 30000 + x * 40000 + x * 70000, SDS-PAGE
Pseudomonas putida
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.8.3.3
30
-
assay at
Pseudomonas putida