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Literature summary extracted from

  • Slabas, A.R.; Kroon, J.T.M.; Scheirer, T.P.; Gilroy, J.S.; Hayman, M.; Rice, D.W.; Turnbull, A.P.; Rafferty, J.B.; Fawcett, T.; Simon, W.J.
    Squash Glycerol-3-phosphate (1)-Acyltransferase. Alteration of substrate selectivity and identification of arginine and lysine residues important in catalytic activity (2002), J. Biol. Chem., 277, 43918-43923.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.15
-
Arabidopsis thaliana
2.3.1.15
-
Cucurbita moschata
2.3.1.15
-
Elaeis guineensis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.15 E142A no activity Cucurbita moschata
2.3.1.15 H194S 79% of wild-type activity Cucurbita moschata
2.3.1.15 K193S no activity Cucurbita moschata
2.3.1.15 L261F 90% of wild-type activity Cucurbita moschata
2.3.1.15 L261F change from a non-selective acyltransferase to an oleate-selective acyltransferase Cucurbita moschata
2.3.1.15 L352F change from a non-selective acyltransferase to an oleate-selective acyltransferase, 117% of wild-type activity Elaeis guineensis
2.3.1.15 R235S no activity Cucurbita moschata
2.3.1.15 R237S no activity Cucurbita moschata
2.3.1.15 S331P no change in acyl-donor selectivity Cucurbita moschata
2.3.1.15 S331P 94% of wild-type activity Cucurbita moschata
2.3.1.15 T141S 106% of wild-type activity Cucurbita moschata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.15 0.0028
-
oleoyl-ACP L261F mutant acyltransferase Cucurbita moschata
2.3.1.15 0.003
-
oleoyl-ACP
-
Cucurbita moschata
2.3.1.15 0.0034
-
palmitoyl-[acyl carrier protein]
-
Cucurbita moschata
2.3.1.15 0.0092
-
palmitoyl-[acyl carrier protein] L261F mutant acyltransferase Cucurbita moschata
2.3.1.15 0.11
-
glycerol 3-phosphate L261F mutant acyltransferase, acyl-donor oleoyl-CoA Cucurbita moschata
2.3.1.15 0.117 0.143 glycerol 3-phosphate acyl donor palmitoyl-CoA Cucurbita moschata
2.3.1.15 0.12
-
glycerol 3-phosphate acyl donor oleoyl-CoA Cucurbita moschata
2.3.1.15 0.143
-
glycerol 3-phosphate acyl donor palmitoyl-CoA Cucurbita moschata
2.3.1.15 0.15
-
glycerol 3-phosphate L261F mutant acyltransferase, acyl-donor palmitoyl-CoA Cucurbita moschata

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Arabidopsis thaliana
-
-
-
2.3.1.15 Cucurbita moschata
-
-
-
2.3.1.15 Elaeis guineensis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate
-
Cucurbita moschata CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate preferred over palmitoyl-CoA Arabidopsis thaliana CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 palmitoyl-[acyl carrier protein] + sn-glycerol 3-phosphate
-
Cucurbita moschata holo-[acyl-carrier protein] + 1-palmitoylglycerol 3-phosphate
-
?