BRENDA - Enzyme Database

Purification and characterization of glycerophosphate acyltransferase from rat liver mitochondria

Vancura, A.; Haldar, D.; J. Biol. Chem. 269, 27209-27215 (1994)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.15
cardiolipin
activation of reconstituted mitochondrial enzyme
Rattus norvegicus
2.3.1.15
Lubrol Px
above the critical micellar concentration
Rattus norvegicus
2.3.1.15
phosphatidylcholine
i.e. lecithin, activation of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
phosphatidylethanolamine
activation of reconstituted mitochondrial acyl transferase
Rattus norvegicus
2.3.1.15
phosphatidylglycerol
highest activation of reconstituted mitochondrial acyltransferase in the presence of Lubrol PX
Rattus norvegicus
2.3.1.15
phosphatidylinositol
activation of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
phosphatidylserine
activation of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
Zwittergent 3-14
above the critical micellar concentration
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.15
3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonic acid
trivial name CHAPS, complete inhibition
Rattus norvegicus
2.3.1.15
cholate
complete inhibition
Rattus norvegicus
2.3.1.15
deoxycholate
complete inhibition
Rattus norvegicus
2.3.1.15
diacylglycerophosphate
-
Rattus norvegicus
2.3.1.15
Monoacylglycerophosphate
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.15
0.0031
-
palmitoyl-CoA
mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol
Rattus norvegicus
2.3.1.15
0.006
-
palmitoyl-CoA
mitochondrial acyl transferase reconstituted in asolectin
Rattus norvegicus
2.3.1.15
0.0064
-
stearoyl-CoA
mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol
Rattus norvegicus
2.3.1.15
0.007
-
palmitoyl-CoA
mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol
Rattus norvegicus
2.3.1.15
0.0084
-
oleoyl-CoA
mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol
Rattus norvegicus
2.3.1.15
0.011
-
stearoyl-CoA
mitochondrial acyl transferase reconstituted in asolectin
Rattus norvegicus
2.3.1.15
0.012
-
myristoyl-CoA
mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol
Rattus norvegicus
2.3.1.15
0.013
-
oleoyl-CoA
mitochondrial acyl transferase reconstituted in asolectin
Rattus norvegicus
2.3.1.15
0.013
-
stearoyl-CoA
mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol
Rattus norvegicus
2.3.1.15
0.015
-
oleoyl-CoA
mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol
Rattus norvegicus
2.3.1.15
0.016
-
myristoyl-CoA
mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol; mitochondrial acyl transferase reconstituted in asolectin
Rattus norvegicus
2.3.1.15
0.46
-
glycerol 3-phosphate
mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol
Rattus norvegicus
2.3.1.15
0.82
-
glycerol 3-phosphate
mitochondrial acyl transferase reconstituted in asolectin
Rattus norvegicus
2.3.1.15
0.93
-
glycerol 3-phosphate
mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
mitochondrial outer membrane
-
Rattus norvegicus
5741
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.15
85000
-
1 * 85000, mitochondrial acyltransferase, SDS-PAGE
Rattus norvegicus
2.3.1.15
95000
105000
mitochondrial acyltransferase, gel filtration
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.15
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.15
mitochondrial acyltransferase, Bio-Gel HAT, Rotofor, Sephacryl S-300
Rattus norvegicus
Renatured (Commentary)
EC Number
Commentary
Organism
2.3.1.15
reconstitution in asolection or phosphatidylglycerol/phosphatidylethanolamine mixture
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.15
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.15
0.043
-
mitochondrial acyltransferase reconstituted in asolectin
Rattus norvegicus
2.3.1.15
0.19
-
mitochondrial acyltransferase reconstituted in phosphatidylglycerol/phosphatidylethanolamine mixture
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
myristoyl-CoA + sn-glycerol 3-phosphate
-
486378
Rattus norvegicus
CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
-
-
-
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
-
486378
Rattus norvegicus
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
-
-
-
2.3.1.15
stearoyl-CoA + sn-glycerol 3-phosphate
-
486378
Rattus norvegicus
CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.15
monomer
1 * 85000, mitochondrial acyltransferase, SDS-PAGE
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.15
cardiolipin
activation of reconstituted mitochondrial enzyme
Rattus norvegicus
2.3.1.15
Lubrol Px
above the critical micellar concentration
Rattus norvegicus
2.3.1.15
phosphatidylcholine
i.e. lecithin, activation of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
phosphatidylethanolamine
activation of reconstituted mitochondrial acyl transferase
Rattus norvegicus
2.3.1.15
phosphatidylglycerol
highest activation of reconstituted mitochondrial acyltransferase in the presence of Lubrol PX
Rattus norvegicus
2.3.1.15
phosphatidylinositol
activation of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
phosphatidylserine
activation of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
Zwittergent 3-14
above the critical micellar concentration
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.15
3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonic acid
trivial name CHAPS, complete inhibition
Rattus norvegicus
2.3.1.15
cholate
complete inhibition
Rattus norvegicus
2.3.1.15
deoxycholate
complete inhibition
Rattus norvegicus
2.3.1.15
diacylglycerophosphate
-
Rattus norvegicus
2.3.1.15
Monoacylglycerophosphate
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.15
0.0031
-
palmitoyl-CoA
mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol
Rattus norvegicus
2.3.1.15
0.006
-
palmitoyl-CoA
mitochondrial acyl transferase reconstituted in asolectin
Rattus norvegicus
2.3.1.15
0.0064
-
stearoyl-CoA
mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol
Rattus norvegicus
2.3.1.15
0.007
-
palmitoyl-CoA
mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol
Rattus norvegicus
2.3.1.15
0.0084
-
oleoyl-CoA
mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol
Rattus norvegicus
2.3.1.15
0.011
-
stearoyl-CoA
mitochondrial acyl transferase reconstituted in asolectin
Rattus norvegicus
2.3.1.15
0.012
-
myristoyl-CoA
mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol
Rattus norvegicus
2.3.1.15
0.013
-
oleoyl-CoA
mitochondrial acyl transferase reconstituted in asolectin
Rattus norvegicus
2.3.1.15
0.013
-
stearoyl-CoA
mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol
Rattus norvegicus
2.3.1.15
0.015
-
oleoyl-CoA
mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol
Rattus norvegicus
2.3.1.15
0.016
-
myristoyl-CoA
mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol; mitochondrial acyl transferase reconstituted in asolectin
Rattus norvegicus
2.3.1.15
0.46
-
glycerol 3-phosphate
mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol
Rattus norvegicus
2.3.1.15
0.82
-
glycerol 3-phosphate
mitochondrial acyl transferase reconstituted in asolectin
Rattus norvegicus
2.3.1.15
0.93
-
glycerol 3-phosphate
mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
mitochondrial outer membrane
-
Rattus norvegicus
5741
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.15
85000
-
1 * 85000, mitochondrial acyltransferase, SDS-PAGE
Rattus norvegicus
2.3.1.15
95000
105000
mitochondrial acyltransferase, gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.15
mitochondrial acyltransferase, Bio-Gel HAT, Rotofor, Sephacryl S-300
Rattus norvegicus
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.15
reconstitution in asolection or phosphatidylglycerol/phosphatidylethanolamine mixture
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.15
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.15
0.043
-
mitochondrial acyltransferase reconstituted in asolectin
Rattus norvegicus
2.3.1.15
0.19
-
mitochondrial acyltransferase reconstituted in phosphatidylglycerol/phosphatidylethanolamine mixture
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
myristoyl-CoA + sn-glycerol 3-phosphate
-
486378
Rattus norvegicus
CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
-
-
-
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
-
486378
Rattus norvegicus
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
-
-
-
2.3.1.15
stearoyl-CoA + sn-glycerol 3-phosphate
-
486378
Rattus norvegicus
CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.15
monomer
1 * 85000, mitochondrial acyltransferase, SDS-PAGE
Rattus norvegicus