EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.15 | bovine serum albumin | alleviates detergent effect of palmitoyl-CoA | Escherichia coli | |
2.3.1.15 | bovine serum albumin | not necessary with acyl-ACP derivatives or mixed micellar samples | Escherichia coli | |
2.3.1.15 | bovine serum albumin | alleviates detergent effect of palmitoyl-CoA and oleoyl-CoA | Escherichia coli | |
2.3.1.15 | phosphatidylethanolamine | inefficient, pH-dependent | Escherichia coli | |
2.3.1.15 | phosphatidylglycerol | - |
Escherichia coli | |
2.3.1.15 | Phospholipids | phosphatidylethanolamine/phosphatidylglycerol/cardiolipin, ratio 6/1/1 | Escherichia coli |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.15 | plsB structural gene encoding glycerol 3-phosphate acyltransferase | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.15 | mitochondrial inner membrane | - |
Escherichia coli | 5743 | - |
2.3.1.15 | plasma membrane | cytoplasmic side | Escherichia coli | 5886 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.15 | additional information | not activated by divalent ions | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.15 | acyl-CoA + sn-glycerol 3-phosphate | Escherichia coli | initial step in membrane phospholipid biosynthesis | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.15 | Escherichia coli | - |
overproducing strains VL3/pVL1, contains hybrid plasmid bearing plsB structural gene and VL3/plB3-4 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.15 | Matrex Gel Red A, octyl-Sepharose CL-4B, hydroxylapatite | Escherichia coli |
2.3.1.15 | solubilized, by non-ionic detergents | Escherichia coli |
2.3.1.15 | solubilized, by non-ionic detergents and reconstituted in unilamellar phospholipid vesicles | Escherichia coli |
EC Number | Renatured (Comment) | Organism |
---|---|---|
2.3.1.15 | reconstituted in phospholipid vesicles containing phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglecerol in the approx. molar ratio of 6:1:1 | Escherichia coli |
2.3.1.15 | reconstitution in cardiolipin/C12E8, i.e. non-ionic detergent, ratio 2:1 mixed micelles | Escherichia coli |
2.3.1.15 | reconstitution in vesicles | Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.1.15 | -70°C, solubilized, indefinitely stable | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.15 | acyl-CoA + sn-glycerol 3-phosphate | dihydroxyacetone phosphate, ethylene glycol phosphate and 1,3-propanediol phosphate can replace glycerol 3-phosphate | Escherichia coli | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.15 | acyl-CoA + sn-glycerol 3-phosphate | exclusively acylates glycerol 3-phosphate at the C-1-position | Escherichia coli | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.15 | acyl-CoA + sn-glycerol 3-phosphate | initial step in membrane phospholipid biosynthesis | Escherichia coli | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.15 | acyl-[acyl-carrier protein] + sn-glycerol 3-phosphate | - |
Escherichia coli | acyl-carrier protein + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.15 | cis-vaccenoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate | - |
Escherichia coli | acyl-carrier protein + 1-cis-vaccenoyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.15 | oleoyl-CoA + sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.15 | palmitoyl-CoA + sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.15 | palmitoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate | - |
Escherichia coli | acyl-carrier protein + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.15 | ? | - |
Escherichia coli |
2.3.1.15 | More | membranes of overproducing Escherichia coli strain VL3/pLB3-4 contain predominantly the dimeric form | Escherichia coli |