BRENDA - Enzyme Database

Glycerophosphate acyltransferase from Escherichia coli

Scheideler, M.A.; Bell, R.M.; Methods Enzymol. 209, 55-63 (1992)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.15
bovine serum albumin
alleviates detergent effect of palmitoyl-CoA; alleviates detergent effect of palmitoyl-CoA and oleoyl-CoA; not necessary with acyl-ACP derivatives or mixed micellar samples
Escherichia coli
2.3.1.15
phosphatidylethanolamine
inefficient, pH-dependent
Escherichia coli
2.3.1.15
phosphatidylglycerol
-
Escherichia coli
2.3.1.15
Phospholipids
phosphatidylethanolamine/phosphatidylglycerol/cardiolipin, ratio 6/1/1
Escherichia coli
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.3.1.15
plsB structural gene encoding glycerol 3-phosphate acyltransferase
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
mitochondrial inner membrane
-
Escherichia coli
5743
-
2.3.1.15
plasma membrane
cytoplasmic side
Escherichia coli
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.15
additional information
not activated by divalent ions
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
Escherichia coli
initial step in membrane phospholipid biosynthesis
CoA + 1-acyl-sn-glycerol 3-phosphate
-
Escherichia coli
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.15
Escherichia coli
-
overproducing strains VL3/pVL1, contains hybrid plasmid bearing plsB structural gene and VL3/plB3-4
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.3.1.15
Matrex Gel Red A, octyl-Sepharose CL-4B, hydroxylapatite; solubilized, by non-ionic detergents; solubilized, by non-ionic detergents and reconstituted in unilamellar phospholipid vesicles
Escherichia coli
Renatured (Commentary)
EC Number
Renatured (Commentary)
Organism
2.3.1.15
reconstituted in phospholipid vesicles containing phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglecerol in the approx. molar ratio of 6:1:1; reconstitution in cardiolipin/C12E8, i.e. non-ionic detergent, ratio 2:1 mixed micelles; reconstitution in vesicles
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.15
-70C, solubilized, indefinitely stable
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
dihydroxyacetone phosphate, ethylene glycol phosphate and 1,3-propanediol phosphate can replace glycerol 3-phosphate
486372
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486372
Escherichia coli
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
exclusively acylates glycerol 3-phosphate at the C-1-position
486372
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486372
Escherichia coli
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
initial step in membrane phospholipid biosynthesis
486372
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486372
Escherichia coli
?
2.3.1.15
acyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
486372
Escherichia coli
acyl-carrier protein + 1-acyl-sn-glycerol 3-phosphate
-
486372
Escherichia coli
?
2.3.1.15
cis-vaccenoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
486372
Escherichia coli
acyl-carrier protein + 1-cis-vaccenoyl-sn-glycerol 3-phosphate
-
486372
Escherichia coli
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
-
486372
Escherichia coli
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486372
Escherichia coli
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
-
486372
Escherichia coli
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486372
Escherichia coli
?
2.3.1.15
palmitoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
486372
Escherichia coli
acyl-carrier protein + 1-palmitoyl-sn-glycerol 3-phosphate
-
486372
Escherichia coli
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.15
?
-
Escherichia coli
2.3.1.15
More
membranes of overproducing Escherichia coli strain VL3/pLB3-4 contain predominantly the dimeric form
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.15
bovine serum albumin
alleviates detergent effect of palmitoyl-CoA; alleviates detergent effect of palmitoyl-CoA and oleoyl-CoA; not necessary with acyl-ACP derivatives or mixed micellar samples
Escherichia coli
2.3.1.15
phosphatidylethanolamine
inefficient, pH-dependent
Escherichia coli
2.3.1.15
phosphatidylglycerol
-
Escherichia coli
2.3.1.15
Phospholipids
phosphatidylethanolamine/phosphatidylglycerol/cardiolipin, ratio 6/1/1
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.15
plsB structural gene encoding glycerol 3-phosphate acyltransferase
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
mitochondrial inner membrane
-
Escherichia coli
5743
-
2.3.1.15
plasma membrane
cytoplasmic side
Escherichia coli
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.15
additional information
not activated by divalent ions
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
Escherichia coli
initial step in membrane phospholipid biosynthesis
CoA + 1-acyl-sn-glycerol 3-phosphate
-
Escherichia coli
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.15
Matrex Gel Red A, octyl-Sepharose CL-4B, hydroxylapatite; solubilized, by non-ionic detergents; solubilized, by non-ionic detergents and reconstituted in unilamellar phospholipid vesicles
Escherichia coli
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.15
reconstituted in phospholipid vesicles containing phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglecerol in the approx. molar ratio of 6:1:1; reconstitution in cardiolipin/C12E8, i.e. non-ionic detergent, ratio 2:1 mixed micelles; reconstitution in vesicles
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.15
-70C, solubilized, indefinitely stable
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
dihydroxyacetone phosphate, ethylene glycol phosphate and 1,3-propanediol phosphate can replace glycerol 3-phosphate
486372
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486372
Escherichia coli
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
exclusively acylates glycerol 3-phosphate at the C-1-position
486372
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486372
Escherichia coli
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
initial step in membrane phospholipid biosynthesis
486372
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486372
Escherichia coli
?
2.3.1.15
acyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
486372
Escherichia coli
acyl-carrier protein + 1-acyl-sn-glycerol 3-phosphate
-
486372
Escherichia coli
?
2.3.1.15
cis-vaccenoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
486372
Escherichia coli
acyl-carrier protein + 1-cis-vaccenoyl-sn-glycerol 3-phosphate
-
486372
Escherichia coli
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
-
486372
Escherichia coli
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486372
Escherichia coli
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
-
486372
Escherichia coli
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486372
Escherichia coli
?
2.3.1.15
palmitoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
486372
Escherichia coli
acyl-carrier protein + 1-palmitoyl-sn-glycerol 3-phosphate
-
486372
Escherichia coli
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.15
?
-
Escherichia coli
2.3.1.15
More
membranes of overproducing Escherichia coli strain VL3/pLB3-4 contain predominantly the dimeric form
Escherichia coli