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Literature summary extracted from

  • Scheideler, M.A.; Bell, R.M.
    Glycerophosphate acyltransferase from Escherichia coli (1992), Methods Enzymol., 209, 55-63.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.15 bovine serum albumin alleviates detergent effect of palmitoyl-CoA Escherichia coli
2.3.1.15 bovine serum albumin not necessary with acyl-ACP derivatives or mixed micellar samples Escherichia coli
2.3.1.15 bovine serum albumin alleviates detergent effect of palmitoyl-CoA and oleoyl-CoA Escherichia coli
2.3.1.15 phosphatidylethanolamine inefficient, pH-dependent Escherichia coli
2.3.1.15 phosphatidylglycerol
-
Escherichia coli
2.3.1.15 Phospholipids phosphatidylethanolamine/phosphatidylglycerol/cardiolipin, ratio 6/1/1 Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.15 plsB structural gene encoding glycerol 3-phosphate acyltransferase Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 mitochondrial inner membrane
-
Escherichia coli 5743
-
2.3.1.15 plasma membrane cytoplasmic side Escherichia coli 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.15 additional information not activated by divalent ions Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Escherichia coli initial step in membrane phospholipid biosynthesis CoA + 1-acyl-sn-glycerol 3-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Escherichia coli
-
overproducing strains VL3/pVL1, contains hybrid plasmid bearing plsB structural gene and VL3/plB3-4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15 Matrex Gel Red A, octyl-Sepharose CL-4B, hydroxylapatite Escherichia coli
2.3.1.15 solubilized, by non-ionic detergents Escherichia coli
2.3.1.15 solubilized, by non-ionic detergents and reconstituted in unilamellar phospholipid vesicles Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.15 reconstituted in phospholipid vesicles containing phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglecerol in the approx. molar ratio of 6:1:1 Escherichia coli
2.3.1.15 reconstitution in cardiolipin/C12E8, i.e. non-ionic detergent, ratio 2:1 mixed micelles Escherichia coli
2.3.1.15 reconstitution in vesicles Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.3.1.15 -70°C, solubilized, indefinitely stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate dihydroxyacetone phosphate, ethylene glycol phosphate and 1,3-propanediol phosphate can replace glycerol 3-phosphate Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate exclusively acylates glycerol 3-phosphate at the C-1-position Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate initial step in membrane phospholipid biosynthesis Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 acyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
Escherichia coli acyl-carrier protein + 1-acyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 cis-vaccenoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
Escherichia coli acyl-carrier protein + 1-cis-vaccenoyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate
-
Escherichia coli CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate
-
Escherichia coli CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?
2.3.1.15 palmitoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
Escherichia coli acyl-carrier protein + 1-palmitoyl-sn-glycerol 3-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.15 ?
-
Escherichia coli
2.3.1.15 More membranes of overproducing Escherichia coli strain VL3/pLB3-4 contain predominantly the dimeric form Escherichia coli