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Literature summary extracted from

  • Snider, M.D.
    sn-Glycero-3-phosphate acyltransferase from Escherichia coli (1981), Methods Enzymol., 71, 546-550.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.15 Phospholipids absolutely required Escherichia coli
2.3.1.15 Phospholipids bilayers required for activity: phosphatidylethanolamine/-glycerol, ratio 4/1, phosphatidylethanolamine/cardiolipin, ratio 4/1 Escherichia coli

General Stability

EC Number General Stability Organism
2.3.1.15 400 mM NaCl or KCl, 5 mM 2-mercaptoethanol, 5 mM MgCl2, stabilize membrane-bound or reconstituted enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.15 0.05
-
 sn-glycerol 3-phosphate acyl-CoA Escherichia coli
2.3.1.15 0.07
-
 acyl-ACP
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 plasma membrane
-
 Escherichia coli 5886
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Escherichia coli
-
 K-12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15 partial purification Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.15 reconstituted at a phospholipid-detergent ratio of 20:1 Escherichia coli
2.3.1.15 reconstitution in vesicles Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.15 0.105
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.3.1.15 -20°C, at least 6 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate exclusively acylates glycerol 3-phosphate at the C-1-position Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate dihydroxyacetone phosphate is ineffective as acyl-acceptor Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate saturated acyl-CoAs preferred Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
 Escherichia coli acyl-carrier protein + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.15 8.5
-
-
 Escherichia coli