BRENDA - Enzyme Database

Partial purification of glycerophosphate acyltransferase from Escherichia coli

Snider, M.D.; Kennedy, E.P.; J. Bacteriol. 130, 1072-1083 (1977)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.15
phosphatidylethanolamine
seems to be required for optimal activity
Escherichia coli
2.3.1.15
Phospholipids
absolutely required; bilayers required for activity: phosphatidylethanolamine/-glycerol, ratio 4/1, phosphatidylethanolamine/cardiolipin, ratio 4/1
Escherichia coli
General Stability
EC Number
General Stability
Organism
2.3.1.15
400 mM NaCl or KCl, 5 mM 2-mercaptoethanol, 5 mM MgCl2, stabilize membrane-bound or reconstituted enzyme
Escherichia coli
2.3.1.15
glycerol, 20%, stabilizes
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.15
cholate
phospholipids restore activity
Escherichia coli
2.3.1.15
deoxycholate
phospholipids restore activity
Escherichia coli
2.3.1.15
Detergents
e.g. Brij 35, Brij 58, Lubrol 17A-10, phospholipids restore activity
Escherichia coli
2.3.1.15
Triton X-100
phospholipids restore activity
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.15
0.56
-
sn-glycerol 3-phosphate
strain 8
Escherichia coli
2.3.1.15
3
3.2
sn-glycerol 3-phosphate
strain BB26-36
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
plasma membrane
-
Escherichia coli
5886
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.15
Escherichia coli
-
A324 and 205; K-12; K-12 derivative strains 8, wild-type genotype; overproducing mutants CV15, lsA-genotype and BB 26-36, plsB-genotype
-
2.3.1.15
Escherichia coli 8
-
overproducing mutants CV15, lsA-genotype and BB 26-36, plsB-genotype
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.15
partial purification
Escherichia coli
Renatured (Commentary)
EC Number
Commentary
Organism
2.3.1.15
reconstitution in phospholipid bilayers
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.15
0.105
-
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
exclusively acylates glycerol 3-phosphate at the C-1-position
486367
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
saturated acyl-CoAs preferred
486367
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
exclusively acylates glycerol 3-phosphate at the C-1-position
486367
Escherichia coli 8
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
saturated acyl-CoAs preferred
486367
Escherichia coli 8
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
16% of the reaction rate with palmitoyl-CoA
486367
Escherichia coli
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486367
Escherichia coli
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
16% of the reaction rate with palmitoyl-CoA
486367
Escherichia coli 8
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486367
Escherichia coli 8
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
4fold better substrate than oleoyl-CoA
486367
Escherichia coli
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486367
Escherichia coli
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
i.e. n-hexadecanoyl-CoA, best substrate
486367
Escherichia coli
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486367
Escherichia coli
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
4fold better substrate than oleoyl-CoA
486367
Escherichia coli 8
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486367
Escherichia coli 8
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
i.e. n-hexadecanoyl-CoA, best substrate
486367
Escherichia coli 8
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486367
Escherichia coli 8
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.15
phosphatidylethanolamine
seems to be required for optimal activity
Escherichia coli
2.3.1.15
Phospholipids
absolutely required; bilayers required for activity: phosphatidylethanolamine/-glycerol, ratio 4/1, phosphatidylethanolamine/cardiolipin, ratio 4/1
Escherichia coli
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.15
400 mM NaCl or KCl, 5 mM 2-mercaptoethanol, 5 mM MgCl2, stabilize membrane-bound or reconstituted enzyme
Escherichia coli
2.3.1.15
glycerol, 20%, stabilizes
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.15
cholate
phospholipids restore activity
Escherichia coli
2.3.1.15
deoxycholate
phospholipids restore activity
Escherichia coli
2.3.1.15
Detergents
e.g. Brij 35, Brij 58, Lubrol 17A-10, phospholipids restore activity
Escherichia coli
2.3.1.15
Triton X-100
phospholipids restore activity
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.15
0.56
-
sn-glycerol 3-phosphate
strain 8
Escherichia coli
2.3.1.15
3
3.2
sn-glycerol 3-phosphate
strain BB26-36
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
plasma membrane
-
Escherichia coli
5886
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.15
partial purification
Escherichia coli
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.15
reconstitution in phospholipid bilayers
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.15
0.105
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
exclusively acylates glycerol 3-phosphate at the C-1-position
486367
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
saturated acyl-CoAs preferred
486367
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
exclusively acylates glycerol 3-phosphate at the C-1-position
486367
Escherichia coli 8
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
saturated acyl-CoAs preferred
486367
Escherichia coli 8
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
16% of the reaction rate with palmitoyl-CoA
486367
Escherichia coli
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486367
Escherichia coli
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
16% of the reaction rate with palmitoyl-CoA
486367
Escherichia coli 8
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486367
Escherichia coli 8
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
4fold better substrate than oleoyl-CoA
486367
Escherichia coli
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486367
Escherichia coli
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
i.e. n-hexadecanoyl-CoA, best substrate
486367
Escherichia coli
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486367
Escherichia coli
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
4fold better substrate than oleoyl-CoA
486367
Escherichia coli 8
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486367
Escherichia coli 8
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
i.e. n-hexadecanoyl-CoA, best substrate
486367
Escherichia coli 8
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486367
Escherichia coli 8
?