BRENDA - Enzyme Database

Function of phospholipids on the regulatory properties of solubilized and membrane-bound sn-glycerol-3-phosphate acyltransferase of Escherichia coli

Kito, M.; Ishinaga, M.; Nishihara, M.; Biochim. Biophys. Acta 529, 237-249 (1978)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.15
additional information
not activated by 1,2-diacyl glycerol 3-phosphate
Escherichia coli
2.3.1.15
phosphatidylglycerol
1.5 mM, 30-40fold activation of solubilized enzyme, inhibiton above
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.15
cis-vaccenoyl-CoA
-
Escherichia coli
2.3.1.15
phosphatidylethanolamine
1,2-unsaturated species
Escherichia coli
2.3.1.15
phosphatidylglycerol
above 1.5 mM, activation below
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.15
0.0056
-
palmitoyl-CoA
strain 8, membrane-associated enzyme
Escherichia coli
2.3.1.15
0.006
-
palmitoyl-CoA
strain VL3/pVL1 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme
Escherichia coli
2.3.1.15
0.0078
-
palmitoyl-CoA
strain VL3/pLB3-4 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme
Escherichia coli
2.3.1.15
0.102
-
sn-glycerol 3-phosphate
strain VL3/pVL1 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme
Escherichia coli
2.3.1.15
0.105
-
sn-glycerol 3-phosphate
strain 8, membrane-associated enzyme
Escherichia coli
2.3.1.15
0.108
-
sn-glycerol 3-phosphate
strain VL3/pLB3-4 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme
Escherichia coli
2.3.1.15
0.156
-
sn-glycerol 3-phosphate
membrane-bound enzyme
Escherichia coli
2.3.1.15
3
3.2
sn-glycerol 3-phosphate
reconstituted enzyme
Escherichia coli
2.3.1.15
3
-
sn-glycerol 3-phosphate
solubilized enzyme in the presence of phosphatidylglycerol
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
plasma membrane
-
Escherichia coli
5886
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.15
Escherichia coli
-
B
-
2.3.1.15
Escherichia coli B / ATCC 11303
-
B
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.15
solubilized
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
cis-vaccenoyl-CoA + sn-glycerol 3-phosphate
-
486363
Escherichia coli
CoA + cis-vaccenoyl-sn-glycerol 3-phosphate
-
486363
Escherichia coli
?
2.3.1.15
cis-vaccenoyl-CoA + sn-glycerol 3-phosphate
-
486363
Escherichia coli B / ATCC 11303
CoA + cis-vaccenoyl-sn-glycerol 3-phosphate
-
486363
Escherichia coli B / ATCC 11303
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
i.e. n-hexadecanoyl-CoA, best substrate
486363
Escherichia coli
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486363
Escherichia coli
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
i.e. n-hexadecanoyl-CoA, best substrate
486363
Escherichia coli B / ATCC 11303
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486363
Escherichia coli B / ATCC 11303
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.15
additional information
not activated by 1,2-diacyl glycerol 3-phosphate
Escherichia coli
2.3.1.15
phosphatidylglycerol
1.5 mM, 30-40fold activation of solubilized enzyme, inhibiton above
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.15
cis-vaccenoyl-CoA
-
Escherichia coli
2.3.1.15
phosphatidylethanolamine
1,2-unsaturated species
Escherichia coli
2.3.1.15
phosphatidylglycerol
above 1.5 mM, activation below
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.15
0.0056
-
palmitoyl-CoA
strain 8, membrane-associated enzyme
Escherichia coli
2.3.1.15
0.006
-
palmitoyl-CoA
strain VL3/pVL1 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme
Escherichia coli
2.3.1.15
0.0078
-
palmitoyl-CoA
strain VL3/pLB3-4 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme
Escherichia coli
2.3.1.15
0.102
-
sn-glycerol 3-phosphate
strain VL3/pVL1 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme
Escherichia coli
2.3.1.15
0.105
-
sn-glycerol 3-phosphate
strain 8, membrane-associated enzyme
Escherichia coli
2.3.1.15
0.108
-
sn-glycerol 3-phosphate
strain VL3/pLB3-4 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme
Escherichia coli
2.3.1.15
0.156
-
sn-glycerol 3-phosphate
membrane-bound enzyme
Escherichia coli
2.3.1.15
3
3.2
sn-glycerol 3-phosphate
reconstituted enzyme
Escherichia coli
2.3.1.15
3
-
sn-glycerol 3-phosphate
solubilized enzyme in the presence of phosphatidylglycerol
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
plasma membrane
-
Escherichia coli
5886
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.15
solubilized
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
cis-vaccenoyl-CoA + sn-glycerol 3-phosphate
-
486363
Escherichia coli
CoA + cis-vaccenoyl-sn-glycerol 3-phosphate
-
486363
Escherichia coli
?
2.3.1.15
cis-vaccenoyl-CoA + sn-glycerol 3-phosphate
-
486363
Escherichia coli B / ATCC 11303
CoA + cis-vaccenoyl-sn-glycerol 3-phosphate
-
486363
Escherichia coli B / ATCC 11303
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
i.e. n-hexadecanoyl-CoA, best substrate
486363
Escherichia coli
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486363
Escherichia coli
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
i.e. n-hexadecanoyl-CoA, best substrate
486363
Escherichia coli B / ATCC 11303
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486363
Escherichia coli B / ATCC 11303
?