BRENDA - Enzyme Database

Membrane phospholipid synthesis in Escherichia coli. Purification, reconstitution, and characterization of sn-glycerol-3-phosphate acyltransferase

Green, P.R.; Merrill, A.H.; Bell, R.M.; J. Biol. Chem. 256, 11151-11159 (1981)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.15
bovine serum albumin
alleviates detergent effect of palmitoyl-CoA
Escherichia coli
2.3.1.15
phosphatidylglycerol
-
Escherichia coli
2.3.1.15
Phospholipids
activation, e.g. phosphatidylglycerol and cardiolipin, i.e. diphosphatidyl glycerol, ratio 2/1, protect against inhibition by palmitoyl-CoA; reconstituted activity of Triton X-100-solubilized enzyme, maximal reconstitution at a phospholipid/Triton X-100 ratio of 20/1
Escherichia coli
General Stability
EC Number
General Stability
Organism
2.3.1.15
column elution during chromatography causes aggregation leading to irreversible inactivation
Escherichia coli
2.3.1.15
phospholipids reconstitute Triton X-100-solubilized enzyme
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.15
bovine serum albumin
above 6 mg/ml, at 0.01 mg palmitoyl-CoA depending on palmitoyl-CoA and phospholipid concentration
Escherichia coli
2.3.1.15
Ca2+
in the absence of NaCl
Escherichia coli
2.3.1.15
Mg2+
in the absence of NaCl
Escherichia coli
2.3.1.15
palmitoyl-CoA
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.15
0.002
-
palmitoyl-CoA
-
Escherichia coli
2.3.1.15
0.005
-
oleoyl-CoA
-
Escherichia coli
2.3.1.15
0.015
-
palmitoyl-ACP
-
Escherichia coli
2.3.1.15
0.025
-
vaccenoyl-ACP
-
Escherichia coli
2.3.1.15
0.15
-
sn-glycerol 3-phosphate
acyl donor palmitoyl-CoA or palmitoyl-ACP
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
plasma membrane
integral cytoplasmic membrane protein
Escherichia coli
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.15
Ca2+
inhibitory in the absence of NaCl
Escherichia coli
2.3.1.15
KCl
activation at 200 mM
Escherichia coli
2.3.1.15
LiCl
activation at 200 mM
Escherichia coli
2.3.1.15
Mg2+
inhibitory in the absence of NaCl
Escherichia coli
2.3.1.15
NaCl
activation at 200 mM
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.15
additional information
-
amino acid analysis
Escherichia coli
2.3.1.15
83000
-
x * 83000, SDS-PAGE
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.15
Escherichia coli
-
overproducing strains VL3/pVL1, contains hybrid plasmid bearing plsB structural gene and VL3/plB3-4
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.15
Matrex Gel Red A, octyl-Sepharose CL-4B, hydroxylapatite; Triton X-100-solubilized
Escherichia coli
Renatured (Commentary)
EC Number
Commentary
Organism
2.3.1.15
reconstitution in dispersed E. coli phospholipids
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.15
2.3
8
-
Escherichia coli
2.3.1.15
6.8
-
-
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.15
-80°C, at least 10 months
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
exclusively acylates glycerol 3-phosphate at the C-1-position
486357
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
acyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
486357
Escherichia coli
acyl-carrier protein + 1-acyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
cis-vaccenoyl-CoA + sn-glycerol 3-phosphate
-
486357
Escherichia coli
CoA + cis-vaccenoyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
cis-vaccenoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
486357
Escherichia coli
acyl-carrier protein + 1-cis-vaccenoyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
myristoyl-CoA + sn-glycerol 3-phosphate
i.e. tetradecanoyl-CoA
486357
Escherichia coli
CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
n-dodecanoyl-CoA + sn-glycerol 3-phosphate
-
486357
Escherichia coli
CoA + 1-n-dodecanoyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
-
486357
Escherichia coli
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
palmitoleoyl-CoA + sn-glycerol 3-phosphate
-
486357
Escherichia coli
CoA + 1-palmitoleoyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
-
486357
Escherichia coli
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
palmitoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
486357
Escherichia coli
acyl-carrier protein + 1-palmitoyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
stearoyl-CoA + sn-glycerol 3-phosphate
-
486357
Escherichia coli
CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
undecanoyl-CoA + sn-glycerol 3-phosphate
-
486357
Escherichia coli
CoA + 1-undecanoyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.15
?
x * 83000, SDS-PAGE
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.15
8.5
-
-
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.15
bovine serum albumin
alleviates detergent effect of palmitoyl-CoA
Escherichia coli
2.3.1.15
phosphatidylglycerol
-
Escherichia coli
2.3.1.15
Phospholipids
activation, e.g. phosphatidylglycerol and cardiolipin, i.e. diphosphatidyl glycerol, ratio 2/1, protect against inhibition by palmitoyl-CoA; reconstituted activity of Triton X-100-solubilized enzyme, maximal reconstitution at a phospholipid/Triton X-100 ratio of 20/1
Escherichia coli
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.15
column elution during chromatography causes aggregation leading to irreversible inactivation
Escherichia coli
2.3.1.15
phospholipids reconstitute Triton X-100-solubilized enzyme
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.15
bovine serum albumin
above 6 mg/ml, at 0.01 mg palmitoyl-CoA depending on palmitoyl-CoA and phospholipid concentration
Escherichia coli
2.3.1.15
Ca2+
in the absence of NaCl
Escherichia coli
2.3.1.15
Mg2+
in the absence of NaCl
Escherichia coli
2.3.1.15
palmitoyl-CoA
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.15
0.002
-
palmitoyl-CoA
-
Escherichia coli
2.3.1.15
0.005
-
oleoyl-CoA
-
Escherichia coli
2.3.1.15
0.015
-
palmitoyl-ACP
-
Escherichia coli
2.3.1.15
0.025
-
vaccenoyl-ACP
-
Escherichia coli
2.3.1.15
0.15
-
sn-glycerol 3-phosphate
acyl donor palmitoyl-CoA or palmitoyl-ACP
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
plasma membrane
integral cytoplasmic membrane protein
Escherichia coli
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.15
Ca2+
inhibitory in the absence of NaCl
Escherichia coli
2.3.1.15
KCl
activation at 200 mM
Escherichia coli
2.3.1.15
LiCl
activation at 200 mM
Escherichia coli
2.3.1.15
Mg2+
inhibitory in the absence of NaCl
Escherichia coli
2.3.1.15
NaCl
activation at 200 mM
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.15
additional information
-
amino acid analysis
Escherichia coli
2.3.1.15
83000
-
x * 83000, SDS-PAGE
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.15
Matrex Gel Red A, octyl-Sepharose CL-4B, hydroxylapatite; Triton X-100-solubilized
Escherichia coli
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.15
reconstitution in dispersed E. coli phospholipids
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.15
2.3
8
-
Escherichia coli
2.3.1.15
6.8
-
-
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.15
-80°C, at least 10 months
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
exclusively acylates glycerol 3-phosphate at the C-1-position
486357
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
acyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
486357
Escherichia coli
acyl-carrier protein + 1-acyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
cis-vaccenoyl-CoA + sn-glycerol 3-phosphate
-
486357
Escherichia coli
CoA + cis-vaccenoyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
cis-vaccenoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
486357
Escherichia coli
acyl-carrier protein + 1-cis-vaccenoyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
myristoyl-CoA + sn-glycerol 3-phosphate
i.e. tetradecanoyl-CoA
486357
Escherichia coli
CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
n-dodecanoyl-CoA + sn-glycerol 3-phosphate
-
486357
Escherichia coli
CoA + 1-n-dodecanoyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
-
486357
Escherichia coli
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
palmitoleoyl-CoA + sn-glycerol 3-phosphate
-
486357
Escherichia coli
CoA + 1-palmitoleoyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
-
486357
Escherichia coli
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
palmitoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
486357
Escherichia coli
acyl-carrier protein + 1-palmitoyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
stearoyl-CoA + sn-glycerol 3-phosphate
-
486357
Escherichia coli
CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
2.3.1.15
undecanoyl-CoA + sn-glycerol 3-phosphate
-
486357
Escherichia coli
CoA + 1-undecanoyl-sn-glycerol 3-phosphate
-
486357
Escherichia coli
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.15
?
x * 83000, SDS-PAGE
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.15
8.5
-
-
Escherichia coli