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Literature summary extracted from

  • Green, P.R.; Merrill, A.H.; Bell, R.M.
    Membrane phospholipid synthesis in Escherichia coli. Purification, reconstitution, and characterization of sn-glycerol-3-phosphate acyltransferase (1981), J. Biol. Chem., 256, 11151-11159.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.15 bovine serum albumin alleviates detergent effect of palmitoyl-CoA Escherichia coli
2.3.1.15 phosphatidylglycerol
-
 Escherichia coli
2.3.1.15 Phospholipids reconstituted activity of Triton X-100-solubilized enzyme, maximal reconstitution at a phospholipid/Triton X-100 ratio of 20/1 Escherichia coli
2.3.1.15 Phospholipids activation, e.g. phosphatidylglycerol and cardiolipin, i.e. diphosphatidyl glycerol, ratio 2/1, protect against inhibition by palmitoyl-CoA Escherichia coli

General Stability

EC Number General Stability Organism
2.3.1.15 column elution during chromatography causes aggregation leading to irreversible inactivation Escherichia coli
2.3.1.15 phospholipids reconstitute Triton X-100-solubilized enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.15 bovine serum albumin above 6 mg/ml, at 0.01 mg palmitoyl-CoA depending on palmitoyl-CoA and phospholipid concentration Escherichia coli
2.3.1.15 Ca2+ in the absence of NaCl Escherichia coli
2.3.1.15 Mg2+ in the absence of NaCl Escherichia coli
2.3.1.15 palmitoyl-CoA
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.15 0.002
-
 palmitoyl-CoA
-
 Escherichia coli
2.3.1.15 0.005
-
 oleoyl-CoA
-
 Escherichia coli
2.3.1.15 0.015
-
 palmitoyl-[acyl carrier protein]
-
 Escherichia coli
2.3.1.15 0.025
-
 vaccenoyl-[acyl carrier protein]
-
 Escherichia coli
2.3.1.15 0.15
-
 sn-glycerol 3-phosphate acyl donor palmitoyl-CoA or palmitoyl-ACP Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 plasma membrane integral cytoplasmic membrane protein Escherichia coli 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.15 Ca2+ inhibitory in the absence of NaCl Escherichia coli
2.3.1.15 KCl activation at 200 mM Escherichia coli
2.3.1.15 LiCl activation at 200 mM Escherichia coli
2.3.1.15 Mg2+ inhibitory in the absence of NaCl Escherichia coli
2.3.1.15 NaCl activation at 200 mM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.15 additional information
-
 amino acid analysis Escherichia coli
2.3.1.15 83000
-
 x * 83000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Escherichia coli
-
 overproducing strains VL3/pVL1, contains hybrid plasmid bearing plsB structural gene and VL3/plB3-4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15 Matrex Gel Red A, octyl-Sepharose CL-4B, hydroxylapatite Escherichia coli
2.3.1.15 Triton X-100-solubilized Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.15 reconstitution in dispersed E. coli phospholipids Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.15 2.3 8
-
 Escherichia coli
2.3.1.15 6.8
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.3.1.15 -80°C, at least 10 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate exclusively acylates glycerol 3-phosphate at the C-1-position Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
 Escherichia coli acyl-carrier protein + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 cis-vaccenoyl-CoA + sn-glycerol 3-phosphate
-
 Escherichia coli CoA + cis-vaccenoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 cis-vaccenoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
 Escherichia coli acyl-carrier protein + 1-cis-vaccenoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 myristoyl-CoA + sn-glycerol 3-phosphate i.e. tetradecanoyl-CoA Escherichia coli CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 n-dodecanoyl-CoA + sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-n-dodecanoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoleoyl-CoA + sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-palmitoleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-[acyl carrier protein] + sn-glycerol 3-phosphate
-
 Escherichia coli holo-[acyl-carrier protein] + 1-palmitoylglycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
 Escherichia coli acyl-carrier protein + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 stearoyl-CoA + sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 undecanoyl-CoA + sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-undecanoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 vaccenoyl-[acyl carrier protein] + sn-glycerol 3-phosphate
-
 Escherichia coli holo-[acyl-carrier protein] + 1-vaccenoylglycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.15 ? x * 83000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.15 8.5
-
-
 Escherichia coli