Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Frentzen, M.; Heinz, E.; McKeon, T.A.; Stumpf, P.K.
    Specificities and selectivities of glycerol-3-phosphate acyltransferase and monoacylglycerol-3-phosphate acyltransferase from pea and spinach chloroplasts (1983), Eur. J. Biochem., 129, 629-636.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.15 oleate not oleoyl-CoA Pisum sativum
2.3.1.15 oleate not oleoyl-CoA Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.15 0.0003 0.0007 oleoyl-ACP
-
 Spinacia oleracea
2.3.1.15 0.0003 0.0007 oleoyl-ACP
-
 Pisum sativum
2.3.1.15 0.0032
-
 palmitoyl-ACP
-
 Spinacia oleracea
2.3.1.15 0.0033
-
 stearoyl-ACP
-
 Spinacia oleracea
2.3.1.15 0.0056
-
 palmitoyl-ACP
-
 Pisum sativum
2.3.1.15 3 3.2 sn-glycerol 3-phosphate acyl donors: palmitoyl-ACP or stearoyl-ACP Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 chloroplast
-
 Spinacia oleracea 9507
-
2.3.1.15 chloroplast
-
 Pisum sativum 9507
-
2.3.1.51 chloroplast envelope
-
 Spinacia oleracea 9941
-
2.3.1.51 chloroplast envelope
-
 Pisum sativum 9941
-
2.3.1.51 membrane
-
 Spinacia oleracea 16020
-
2.3.1.51 membrane
-
 Pisum sativum 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.51 acyl-[acyl-carrier protein] + 1-acyl-sn-glycerol 3-phosphate Spinacia oleracea involved in phospholipid biosynthesis in chloroplasts ACP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-[acyl-carrier protein] + 1-acyl-sn-glycerol 3-phosphate Pisum sativum involved in phospholipid biosynthesis in chloroplasts ACP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 additional information Spinacia oleracea acyl-ACP rather than acyl-CoA is the physiological acyl donor for glycerolipid biosynthesis in chloroplasts ?
-
 ?
2.3.1.51 additional information Pisum sativum acyl-ACP rather than acyl-CoA is the physiological acyl donor for glycerolipid biosynthesis in chloroplasts ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Pisum sativum
-
 pea, var. kleine Rheinländerin
-
2.3.1.15 Spinacia oleracea
-
 var. Mona Lisa
-
2.3.1.51 Pisum sativum
-
 pea, cv. Kleine Rheinlaenderin
-
2.3.1.51 Spinacia oleracea
-
 spinach, cv. Mona Lisa
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15
-
 Spinacia oleracea
2.3.1.15
-
 Pisum sativum
2.3.1.51 partial Spinacia oleracea
2.3.1.51 partial Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.15 leaf
-
 Spinacia oleracea
-
2.3.1.15 leaf
-
 Pisum sativum
-
2.3.1.51 leaf
-
 Spinacia oleracea
-
2.3.1.51 leaf
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.15 0.66
-
-
 Spinacia oleracea
2.3.1.15 1.74 3.18
-
 Pisum sativum

Storage Stability

EC Number Storage Stability Organism
2.3.1.15 -20°C, 50% glycerol, at least 6 months Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate specific for glycerol 3-phosphate Spinacia oleracea CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate specific for glycerol 3-phosphate Pisum sativum CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate exclusively acylates glycerol 3-phosphate at the C-1-position Spinacia oleracea CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate exclusively acylates glycerol 3-phosphate at the C-1-position Pisum sativum CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate dihydroxyacetone phosphate is ineffective as acyl-acceptor Spinacia oleracea CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate dihydroxyacetone phosphate is ineffective as acyl-acceptor Pisum sativum CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-[acyl-carrier protein] + sn-glycerol 3-phosphate specific for glycerol 3-phosphate, ACP-thioesters preferred over CoA-thioester Spinacia oleracea acyl-carrier protein + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-[acyl-carrier protein] + sn-glycerol 3-phosphate specific for glycerol 3-phosphate, ACP-thioesters preferred over CoA-thioester Pisum sativum acyl-carrier protein + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate
-
 Spinacia oleracea CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate
-
 Pisum sativum CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 oleoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate best substrate Spinacia oleracea acyl-carrier protein + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 oleoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate best substrate Pisum sativum acyl-carrier protein + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
 Spinacia oleracea acyl-carrier protein + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
 Pisum sativum acyl-carrier protein + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 stearoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
 Spinacia oleracea acyl-carrier protein + 1-stearoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 stearoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
 Pisum sativum acyl-carrier protein + 1-stearoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-ACP + 1-acyl-sn-glycerol 3-phosphate catalyzes acylation of position 2 hydroxyl-group, acyl-donor: highly specific for palmitoyl-ACP preferred to oleoyl-ACP, acyl-acceptors are: 1-oleoyl-sn-glycerol 3-phosphate, which is the best and 1-palmitoyl-sn-glycerol 3-phosphate Spinacia oleracea ACP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-ACP + 1-acyl-sn-glycerol 3-phosphate catalyzes acylation of position 2 hydroxyl-group, acyl-donor: highly specific for palmitoyl-ACP preferred to oleoyl-ACP, acyl-acceptors are: 1-oleoyl-sn-glycerol 3-phosphate, which is the best and 1-palmitoyl-sn-glycerol 3-phosphate Pisum sativum ACP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Spinacia oleracea CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Pisum sativum CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 Spinacia oleracea CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 Pisum sativum CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
-
 Spinacia oleracea CoA + ?
-
 ?
2.3.1.51 acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
-
 Pisum sativum CoA + ?
-
 ?
2.3.1.51 acyl-CoA + myristoyl-sn-glycerol 3-phosphate
-
 Spinacia oleracea CoA + ?
-
 ?
2.3.1.51 acyl-CoA + myristoyl-sn-glycerol 3-phosphate
-
 Pisum sativum CoA + ?
-
 ?
2.3.1.51 acyl-[acyl-carrier protein] + 1-acyl-sn-glycerol 3-phosphate involved in phospholipid biosynthesis in chloroplasts Spinacia oleracea ACP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-[acyl-carrier protein] + 1-acyl-sn-glycerol 3-phosphate involved in phospholipid biosynthesis in chloroplasts Pisum sativum ACP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 additional information the enzyme uses preferably ACP-thioesters for the acylation of 1-acylglycerol 3-phosphate, the enzyme does not accept glycerol 3-phosphate as acyl-acceptor Spinacia oleracea ?
-
 ?
2.3.1.51 additional information the enzyme uses preferably ACP-thioesters for the acylation of 1-acylglycerol 3-phosphate, the enzyme does not accept glycerol 3-phosphate as acyl-acceptor Pisum sativum ?
-
 ?
2.3.1.51 additional information no acyl-donors are coenzyme A derivatives Spinacia oleracea ?
-
 ?
2.3.1.51 additional information no acyl-donors are coenzyme A derivatives Pisum sativum ?
-
 ?
2.3.1.51 additional information no acyl acceptor is glycerol 3-phosphate Spinacia oleracea ?
-
 ?
2.3.1.51 additional information no acyl acceptor is glycerol 3-phosphate Pisum sativum ?
-
 ?
2.3.1.51 additional information acyl-ACP rather than acyl-CoA is the physiological acyl donor for glycerolipid biosynthesis in chloroplasts Spinacia oleracea ?
-
 ?
2.3.1.51 additional information acyl-ACP rather than acyl-CoA is the physiological acyl donor for glycerolipid biosynthesis in chloroplasts Pisum sativum ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.51 7.4
-
 assay at Spinacia oleracea
2.3.1.51 7.4
-
 assay at Pisum sativum