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Literature summary extracted from

  • Negrel, J.; Paynot, M.; Javelle, F.
    Purification and properties of putrescine hydroxycinnamoyl transferase from tobacco (Nicotiana tabaccum) cell suspensions (1992), Plant Physiol., 98, 1264-1269.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.138 ammonium sulfate
-
 Nicotiana tabacum
2.3.1.138 additional information no inhibition by thiol reagents Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.138 0.0025
-
 cinnamoyl-CoA with putrescine Nicotiana tabacum
2.3.1.138 0.0125
-
 feruloyl-CoA with caffeoyl-CoA Nicotiana tabacum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.138 45000
-
 HPLC gel filtration Nicotiana tabacum
2.3.1.138 50000
-
 1 * 50000, SDS-PAGE Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.138 caffeoyl-CoA + putrescine Nicotiana tabacum
-
 CoA + N-caffeoylputrescine
-
 ?
2.3.1.138 caffeoyl-CoA + putrescine Nicotiana tabacum TX1
-
 CoA + N-caffeoylputrescine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.138 Nicotiana tabacum
-
 TX1
-
2.3.1.138 Nicotiana tabacum TX1
-
 TX1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.138
-
 Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.138 cell suspension culture
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.138 4-fluorocinnamoyl-CoA + putrescine
-
 Nicotiana tabacum CoA + N-4-fluorocinnamoylputrescine
-
 ?
2.3.1.138 caffeoyl-CoA + cadaverine
-
 Nicotiana tabacum CoA + N-caffeoylcadaverine
-
 ?
2.3.1.138 caffeoyl-CoA + diaminopropane
-
 Nicotiana tabacum CoA + N-caffeoyl-1,3-diaminopropane
-
 ?
2.3.1.138 caffeoyl-CoA + putrescine
-
 Nicotiana tabacum CoA + N-caffeoylputrescine
-
 ?
2.3.1.138 caffeoyl-CoA + putrescine
-
 Nicotiana tabacum TX1 CoA + N-caffeoylputrescine
-
 ?
2.3.1.138 cinnamoyl-CoA + putrescine
-
 Nicotiana tabacum CoA + N-cinnamoylputrescine
-
 ?
2.3.1.138 cinnamoyl-CoA + putrescine
-
 Nicotiana tabacum TX1 CoA + N-cinnamoylputrescine
-
 ?
2.3.1.138 coumaroyl-CoA + putrescine
-
 Nicotiana tabacum CoA + N-coumaroylputrescine
-
 ?
2.3.1.138 feruloyl-CoA + putrescine
-
 Nicotiana tabacum CoA + N-feruloylputrescine
-
 ?
2.3.1.138 feruloyl-CoA + putrescine
-
 Nicotiana tabacum TX1 CoA + N-feruloylputrescine
-
 ?
2.3.1.138 additional information no activity with monoamines Nicotiana tabacum ?
-
 ?
2.3.1.138 additional information no activity with monoamines Nicotiana tabacum TX1 ?
-
 ?
2.3.1.138 sinapoyl-CoA + putrescine
-
 Nicotiana tabacum CoA + N-sinapoylputrescine
-
 ?
2.3.1.138 sinapoyl-CoA + putrescine
-
 Nicotiana tabacum TX1 CoA + N-sinapoylputrescine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.138 monomer 1 * 50000, SDS-PAGE Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.138 8.5
-
-
 Nicotiana tabacum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.138 7.5 11 about 50% of activity maximum at pH 7.5 and 11 Nicotiana tabacum