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Literature summary extracted from

  • Hoffmann, L.; Maury, S.; Martz, F.; Geoffroy, P.; Legrand, M.
    Purification, cloning, and properties of an acyltransferase controlling shikimate and quinate ester intermediates in phenylpropanoid metabolism (2003), J. Biol. Chem., 278, 95-103.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.99 recombinant protein fused to glutathione-S-transferase is expressed in Nicotiana tabacum
2.3.1.133 expression in Escherichia coli Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.99 0.05
-
 caffeoyl-CoA pH 6.6, 30°C, cosubstrate: shikimate Nicotiana tabacum
2.3.1.99 0.15
-
 p-Coumaroyl-CoA pH 6.6, 30°C, cosubstrate: quinate Nicotiana tabacum
2.3.1.99 0.35
-
 feruloyl-CoA pH 6.6, 30°C, cosubstrate: shikimate Nicotiana tabacum
2.3.1.99 0.6
-
 p-Coumaroyl-CoA pH 6.6, 30°C, cosubstrate: shikimate Nicotiana tabacum
2.3.1.99 0.73
-
 caffeoyl-CoA pH 6.6, 30°C, cosubstrate: quinate Nicotiana tabacum
2.3.1.99 0.75
-
 shikimate pH 6.6, 30°C, cosubstrate: p-coumaroyl-CoA Nicotiana tabacum
2.3.1.133 0.05
-
 caffeoyl-CoA
-
 Nicotiana tabacum
2.3.1.133 0.07
-
 quinate
-
 Nicotiana tabacum
2.3.1.133 0.35
-
 feruloyl-CoA
-
 Nicotiana tabacum
2.3.1.133 0.6
-
 4-coumaroyl-CoA
-
 Nicotiana tabacum
2.3.1.133 0.75
-
 shikimate
-
 Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.99 additional information Nicotiana tabacum the enzyme controls shikimate and quinate ester intermediates in phenylpropanoid metabolism ?
-
 ?
2.3.1.133 4-coumaroyl-CoA + shikimate Nicotiana tabacum enzyme appears to play a critical role in the phenylpropanoid pathway, both upstream and downstream of the 3-hydroxylation step CoA + 5-O-(4-coumaroyl)shikimate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.99 Nicotiana tabacum Q8GSM7
-
-
2.3.1.133 Nicotiana tabacum Q8GSM7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.99 recombinant protein fused to glutathione-S-transferase Nicotiana tabacum
2.3.1.133 recombinant enzyme Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.99 stem
-
 Nicotiana tabacum
-
2.3.1.133 stem
-
 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.99 additional information
-
 decrease in absorbance at DELTA346 nM Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.99 caffeoyl-CoA + quinate
-
 Nicotiana tabacum CoA + 5-O-caffeoylquinate
-
 ?
2.3.1.99 caffeoyl-CoA + shikimate caffeoyl-CoA is the most efficient acyl group donor with shikimate as substrate Nicotiana tabacum CoA + 5-O-caffeoylshikimate
-
 ?
2.3.1.99 feruloyl-CoA + shikimate feruloyl-CoA shows 10fold lower activity than caffeoyl-CoA with shikimate as substrate Nicotiana tabacum CoA + 5-O-feruloylshikimate
-
 ?
2.3.1.99 additional information the enzyme controls shikimate and quinate ester intermediates in phenylpropanoid metabolism Nicotiana tabacum ?
-
 ?
2.3.1.99 additional information no activity is detected with quinate and feruloyl-CoA or sinapoyl-CoA Nicotiana tabacum ?
-
 ?
2.3.1.99 p-coumaroyl-CoA + quinate 4-coumaroyl-CoA is the most efficient acyl group donor with quinate as substrate Nicotiana tabacum CoA + 5-O-(p-coumaroyl)quinate
-
 ?
2.3.1.99 p-coumaroyl-CoA + shikimate
-
 Nicotiana tabacum CoA + 5-O-(p-coumaroyl)shikimate
-
 ?
2.3.1.133 4-coumaroyl-CoA + quinate
-
 Nicotiana tabacum CoA + 4-coumaroylquinate
-
 r
2.3.1.133 4-coumaroyl-CoA + shikimate
-
 Nicotiana tabacum CoA + 5-O-(4-coumaroyl)shikimate
-
 r
2.3.1.133 4-coumaroyl-CoA + shikimate enzyme appears to play a critical role in the phenylpropanoid pathway, both upstream and downstream of the 3-hydroxylation step Nicotiana tabacum CoA + 5-O-(4-coumaroyl)shikimate
-
 ?
2.3.1.133 caffeoyl-CoA + shikimate
-
 Nicotiana tabacum CoA + 5-O-caffeoylshikimate
-
 r
2.3.1.133 feruloyl-CoA + shikimate
-
 Nicotiana tabacum CoA + 5-O-feruloylshikimate
-
 r
2.3.1.133 additional information no substrate: anthranilate, glucose, malate, tyramine, spermidine, spermine, putrescine, agmatine, benzyl alcohol Nicotiana tabacum ?
-
 ?