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Literature summary extracted from

  • Gang, D.R.; Beuerle, T.; Ullmann, P.; Werck-Reichhart, D.; Pichersky, E.
    Differential production of meta hydroxylated phenylpropanoids in sweet basil peltate glandular trichomes and leaves is controlled by the activities of specific acyltransferases and hydroxylases (2002), Plant Physiol., 130, 1536-1544.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.133 48000
-
gel filtration Ocimum basilicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.133 4-coumaroyl-CoA + shikimate Ocimum basilicum enzyme appears to play a critical role in the phenylpropanoid pathway, both upstream and downstream of the 3-hydroxylation step CoA + 5-O-(4-coumaroyl)shikimate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.133 Ocimum basilicum
-
SW and EMX-1
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Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.133 partial Ocimum basilicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.133 gland
-
Ocimum basilicum
-
2.3.1.133 leaf
-
Ocimum basilicum
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2.3.1.133 additional information enzyme activity is much higher in tissues that are actively producing eugenol than in tissues that are not Ocimum basilicum
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Storage Stability

EC Number Storage Stability Organism
2.3.1.133 incubation for days at room temperature, multiple freeze/thaw cycles, holding at -20°C for several months, with or without the addition of 10% glycerol, no loss of activity Ocimum basilicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.133 4-coumaroyl-CoA + shikimate
-
Ocimum basilicum CoA + 5-O-(4-coumaroyl)shikimate
-
?
2.3.1.133 4-coumaroyl-CoA + shikimate enzyme appears to play a critical role in the phenylpropanoid pathway, both upstream and downstream of the 3-hydroxylation step Ocimum basilicum CoA + 5-O-(4-coumaroyl)shikimate
-
?
2.3.1.133 caffeoyl-CoA + shikimate the activity is 100fold lower than the activity with coumaroyl-CoA Ocimum basilicum CoA + 5-O-caffeoylshikimate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.133 37
-
enzyme extremely stable Ocimum basilicum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.133 additional information
-
above 40°C rapid and irreversible loss of activity Ocimum basilicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.133 7
-
50 mM potassium phosphate buffer Ocimum basilicum