EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.133 | 0.41 | - |
caffeoyl-CoA | - |
Equisetum arvense | |
2.3.1.133 | 0.5 | - |
4-coumaroyl-CoA | - |
Equisetum arvense | |
2.3.1.133 | 1.1 | - |
3-phenylacryloyl-CoA | - |
Equisetum arvense | |
2.3.1.133 | 1.9 | - |
feruloyl-CoA | - |
Equisetum arvense | |
2.3.1.133 | 4.32 | - |
shikimate | - |
Equisetum arvense | |
2.3.1.133 | 5.5 | - |
shikimate | - |
Equisetum arvense |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.133 | 50000 | - |
gel filtration | Equisetum arvense |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.99 | Equisetum arvense | - |
- |
- |
2.3.1.133 | Equisetum arvense | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.133 | partial | Equisetum arvense |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.99 | additional information | - |
change of enzyme activity during a 1-year growth period | Equisetum arvense |
2.3.1.133 | additional information | - |
change of enzyme activity during a 1-year growth period | Equisetum arvense |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.1.133 | -20°C, for several months with 10-20% loss of apparent activities after thawing | Equisetum arvense |
2.3.1.133 | -80°C, for several months with 10-20% loss of apparent activities after thawing | Equisetum arvense |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.99 | caffeoyl-CoA + quinate | - |
Equisetum arvense | CoA + 5-O-caffeoylquinate | i.e. chlorogenic acid | ? | |
2.3.1.133 | 3-phenylacryloyl-CoA + shikimate | - |
Equisetum arvense | CoA + 3-phenylacryloylshikimate | - |
? | |
2.3.1.133 | 4-coumaroyl-CoA + shikimate | shikimate cannot be replaced by quinate | Equisetum arvense | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
2.3.1.133 | caffeoyl-CoA + shikimate | - |
Equisetum arvense | CoA + 5-O-caffeoylshikimate | - |
? | |
2.3.1.133 | cinnamoyl-CoA + shikimate | - |
Equisetum arvense | CoA + 5-O-cinnamoylshikimate | - |
? | |
2.3.1.133 | feruloyl-CoA + shikimate | - |
Equisetum arvense | CoA + 5-O-feruloylshikimate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.133 | 40 | - |
- |
Equisetum arvense |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.133 | 7.5 | - |
Tris-HCl | Equisetum arvense |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.133 | 6.2 | 8.5 | about half-maximal activity at pH 6.2 in potassium phosphate buffer and 8.5 in Tris-HCl puffer | Equisetum arvense |