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Literature summary extracted from

  • Lotfy, S.; Fleuriet, A.; Macheix, J.J.
    Hydroxycinnamoyl-CoA: transferases in higher plants. II. Characterization in Cichorium endivia and Raphanus sativus and comparison with other plants (1994), Plant Physiol. Biochem., 32, 355-363.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.133 EDTA no effect Raphanus sativus
2.3.1.133 EDTA no effect Cichorium endivia

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.99 Ca2+ inhibition at 5 mM Cichorium endivia
2.3.1.99 DTT concentration above 5 mM results in a marked inhibition of activity Cichorium endivia
2.3.1.99 Mg2+ inhibition at 5 mM Cichorium endivia
2.3.1.133 Ca2+ inhibition at 5 mM Cichorium endivia
2.3.1.133 Ca2+ inhibition at 5 mM Raphanus sativus
2.3.1.133 DTT concentration above 5 mM results in a marked inhibition of activity Cichorium endivia
2.3.1.133 DTT concentration above 5 mM results in a marked inhibition of activity Raphanus sativus
2.3.1.133 Mg2+ inhibition at 5 mM Cichorium endivia
2.3.1.133 Mg2+ inhibition at 5 mM Raphanus sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.99 10
-
 coumaroyl-CoA
-
 Cichorium endivia
2.3.1.99 11.4
-
 feruloyl-CoA
-
 Cichorium endivia
2.3.1.99 14.3
-
 caffeoyl-CoA
-
 Cichorium endivia
2.3.1.133 12.5
-
 caffeoyl-CoA
-
 Cichorium endivia
2.3.1.133 14.3
-
 4-coumaroyl-CoA
-
 Raphanus sativus
2.3.1.133 14.3
-
 4-coumaroyl-CoA
-
 Cichorium endivia
2.3.1.133 15.4
-
 feruloyl-CoA
-
 Cichorium endivia
2.3.1.133 30.5
-
 caffeoyl-CoA
-
 Raphanus sativus
2.3.1.133 55.5
-
 feruloyl-CoA
-
 Raphanus sativus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.133 Ca2+ sligthly stimulated by 3.0 mM, inhibition at 5 mM Raphanus sativus
2.3.1.133 Ca2+ sligthly stimulated by 3.0 mM, inhibition at 5 mM Cichorium endivia
2.3.1.133 Mg2+ slightly stimulated by 0.5 mM, inhibition at 5 mM Raphanus sativus
2.3.1.133 Mg2+ slightly stimulated by 0.5 mM, inhibition at 5 mM Cichorium endivia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.99 40000
-
 gel filtration Cichorium endivia
2.3.1.133 40000
-
 gel filtration Raphanus sativus
2.3.1.133 40000
-
 gel filtration Cichorium endivia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.133 4-coumaroyl-CoA + shikimate Raphanus sativus hydroxycinnamic acid-shikimate ester biosynthesis CoA + 5-O-(4-coumaroyl)shikimate
-
 ?
2.3.1.133 4-coumaroyl-CoA + shikimate Cichorium endivia hydroxycinnamic acid-shikimate ester biosynthesis CoA + 5-O-(4-coumaroyl)shikimate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.99 Cichorium endivia
-
-
-
2.3.1.133 Cichorium endivia
-
-
-
2.3.1.133 Raphanus sativus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.99 partial Cichorium endivia
2.3.1.133
-
 Cichorium endivia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.99 leaf
-
 Cichorium endivia
-
2.3.1.133 leaf
-
 Raphanus sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.133 additional information
-
-
 Cichorium endivia

Storage Stability

EC Number Storage Stability Organism
2.3.1.99 -20°C, for several months, no apparent loss of activity Cichorium endivia
2.3.1.133 -20°C, for several months, no apparent loss of activity Cichorium endivia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.99 4-coumaroyl-CoA + quinate
-
 Cichorium endivia CoA + 5-O-(4-coumaroyl)quinate
-
 ?
2.3.1.99 caffeoyl-CoA + quinate
-
 Cichorium endivia CoA + 5-O-caffeoylquinate
-
 ?
2.3.1.99 feruloyl-CoA + quinate
-
 Cichorium endivia CoA + O-feruloylquinate
-
 ?
2.3.1.133 4-coumaroyl-CoA + quinate
-
 Raphanus sativus CoA + 4-coumaroylquinate
-
 ?
2.3.1.133 4-coumaroyl-CoA + shikimate
-
 Raphanus sativus CoA + 5-O-(4-coumaroyl)shikimate
-
 ?
2.3.1.133 4-coumaroyl-CoA + shikimate
-
 Cichorium endivia CoA + 5-O-(4-coumaroyl)shikimate
-
 ?
2.3.1.133 4-coumaroyl-CoA + shikimate hydroxycinnamic acid-shikimate ester biosynthesis Raphanus sativus CoA + 5-O-(4-coumaroyl)shikimate
-
 ?
2.3.1.133 4-coumaroyl-CoA + shikimate hydroxycinnamic acid-shikimate ester biosynthesis Cichorium endivia CoA + 5-O-(4-coumaroyl)shikimate
-
 ?
2.3.1.133 caffeoyl-CoA + shikimate
-
 Raphanus sativus CoA + 5-O-caffeoylshikimate
-
 ?
2.3.1.133 caffeoyl-CoA + shikimate
-
 Cichorium endivia CoA + 5-O-caffeoylshikimate
-
 ?
2.3.1.133 feruloyl-CoA + shikimate
-
 Raphanus sativus CoA + 5-O-feruloylshikimate
-
 ?
2.3.1.133 feruloyl-CoA + shikimate
-
 Cichorium endivia CoA + 5-O-feruloylshikimate
-
 ?
2.3.1.133 additional information no substrate: cinnamoyl-CoA, L-malate, D(+)-malate, L(+)-tartrate, m-tartrate, citrate, glucose, UDP-glucose, myo-inositol, tyramine, agmatine Raphanus sativus ?
-
 ?
2.3.1.133 additional information no substrate: cinnamoyl-CoA, L-malate, D(+)-malate, L(+)-tartrate, m-tartrate, citrate, glucose, UDP-glucose, myo-inositol, tyramine, agmatine Cichorium endivia ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.99 additional information
-
 pI: 5.1 Cichorium endivia
2.3.1.99 6.5
-
 50 mM potassium phosphate buffer Cichorium endivia
2.3.1.133 6.5
-
 50 mM potassium phosphate buffer Cichorium endivia
2.3.1.133 6.5
-
 pI: 4.33 Cichorium endivia
2.3.1.133 6.8
-
 50 mM potassium phosphate buffer Raphanus sativus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.133 5 9
-
 Raphanus sativus
2.3.1.133 5 9
-
 Cichorium endivia

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.99 Cichorium endivia
-
-
 5.1