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Literature summary extracted from

  • Wyckoff, T.J.O.; Raetz, C.R.H.
    The active site of Escherichia coli UDP-N-acetylglucosamine acyltransferase. Chemical modification and site-directed mutagenesis (1999), J. Biol. Chem., 274, 27047-27055.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.129 H122A lower specific activity than wild-type Escherichia coli
2.3.1.129 H122N lower specific activity than wild-type Escherichia coli
2.3.1.129 H125A lower specific activity than wild-type Escherichia coli
2.3.1.129 H125N lower specific activity than wild-type Escherichia coli
2.3.1.129 H144A lower specific activity than wild-type Escherichia coli
2.3.1.129 H144N lower specific activity than wild-type Escherichia coli
2.3.1.129 H160A lower specific activity than wild-type Escherichia coli
2.3.1.129 H160F lower specific activity than wild-type Escherichia coli
2.3.1.129 K76A lower specific activity than wild-type Escherichia coli
2.3.1.129 K76R lower specific activity than wild-type Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.129 diethyldicarbonate
-
 Escherichia coli
2.3.1.129 Phenylglyoxal
-
 Escherichia coli
2.3.1.129 pyridoxal 5'-phosphate/sodium borohydride
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.129 0.82
-
 UDP-N-acetylglucosamine wild-type Escherichia coli
2.3.1.129 7
-
 UDP-N-acetylglucosamine K76R Escherichia coli
2.3.1.129 15
-
 UDP-N-acetylglucosamine K76A Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.129 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.129 (R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine
-
 Escherichia coli [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.129 trimer
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.129 7.17
-
 UDP-N-acetylglucosamine wild-type Escherichia coli
2.3.1.129 9.83
-
 UDP-N-acetylglucosamine K76R Escherichia coli
2.3.1.129 10.5
-
 UDP-N-acetylglucosamine K76A Escherichia coli