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Literature summary extracted from

  • Anderson, M.S.; Bull, H.G.; Galloway, S.M.; Kelly, T.M.; Mohan, S.; Radika, K.; Raetz, C.R.
    UDP-N-acetylglucosamine acyltransferase of Escherichia coli. The first step of endotoxin biosynthesis is thermodynamically unfavorable (1993), J. Biol. Chem., 268, 19858-19865.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.129 bovine serum albumin prevents denaturation Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.129 KCl 0.5 M Escherichia coli
2.3.1.129 LiCl 0.5 M Escherichia coli
2.3.1.129 additional information no inhibition by N-ethylmaleimide, UDP-2,3-diacyl-N-glucosamine, lipid X, lipid IVA, 3-deoxy-D-mannooctulosonate, octyl-beta-D-glucoside Escherichia coli
2.3.1.129 myristoyl-[acyl-carrier protein]
-
 Escherichia coli
2.3.1.129 NaCl 0.5 M Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.129 0.0016
-
 (R)-3-hydroxytetradecanoyl-[acyl-carrier protein]
-
 Escherichia coli
2.3.1.129 0.099
-
 UDP-N-acetylglucosamine
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.129 soluble
-
 Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.129 KCl slight activation at 0.25 M Escherichia coli
2.3.1.129 LiCl slight activation at 0.25 M Escherichia coli
2.3.1.129 NaCl slight activation at 0.25 M Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.129 27000
-
 x * 27000, calculated from nucleotide sequence Escherichia coli
2.3.1.129 30000
-
 alpha2, 2 * 30000, SDS-PAGE Escherichia coli
2.3.1.129 30000
-
 alpha3, 3 * 30000, SDS-PAGE Escherichia coli
2.3.1.129 90000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.129 Escherichia coli
-
 MC1061/pSR1 (overproducing strain harbouring plasmid pSR1)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.129
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.129 12.3
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.129 (R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine no substrates are UDPglucosamine, UDP-N-(R)-3-hydroxymyristoylglucosamine, ADP-N-acetylglucosamine, GDP-N-acetylglucosamine, CDP-N-acetylglucosamine, (R)-3-hydroxytetradecanoyl-CoA, or palmitoyl-CoA, myristoyl-[acyl-carrier-protein], and palmitoyl-[acyl-carrier-protein] Escherichia coli [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
 r
2.3.1.129 (R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine high specificity with respect to acyl-donor, equilibrium constant favors thioester acyl carrier substrate Escherichia coli [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.129 ? x * 27000, calculated from nucleotide sequence Escherichia coli
2.3.1.129 dimer or trimer alpha2, 2 * 30000, SDS-PAGE Escherichia coli
2.3.1.129 trimer alpha3, 3 * 30000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.129 30
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.129 6.8 8.2 broad Escherichia coli