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Literature summary extracted from

  • Strack, D.; Leicht, P.; Bokern, M.; Wray, V.; Grotjahn, L.
    Hydroxycinnamic acid esters of isocitric acid: Accumulation and enzymatic synthesis in Amaranthus cruentus (1987), Phytochemistry, 26, 2919-2923.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.126 0.045
-
 caffeoyl-CoA
-
 Amaranthus cruentus
2.3.1.126 0.055
-
 4-coumaroyl-CoA
-
 Amaranthus cruentus
2.3.1.126 0.163
-
 feruloyl-CoA
-
 Amaranthus cruentus
2.3.1.126 0.208
-
 isocitric acid second substrate caffeoyl-CoA Amaranthus cruentus
2.3.1.126 0.257
-
 isocitric acid second substrate p-coumaroyl-CoA Amaranthus cruentus
2.3.1.126 0.412
-
 isocitric acid second substrate feruloyl-CoA Amaranthus cruentus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.126 caffeoyl-CoA + isocitrate Amaranthus hybridus
-
 CoA + 2-caffeoylisocitrate
-
 r
2.3.1.126 caffeoyl-CoA + isocitrate Amaranthus tricolor
-
 CoA + 2-caffeoylisocitrate
-
 r
2.3.1.126 caffeoyl-CoA + isocitrate Amaranthus cruentus
-
 CoA + 2-caffeoylisocitrate
-
 r
2.3.1.126 caffeoyl-CoA + isocitrate Amaranthus paniculatus
-
 CoA + 2-caffeoylisocitrate
-
 r
2.3.1.126 caffeoyl-CoA + isocitrate Amaranthus paniculatus L.
-
 CoA + 2-caffeoylisocitrate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.126 Amaranthus cruentus
-
 Willd.
-
2.3.1.126 Amaranthus cruentus
-
 L. cv. Oeschberg
-
2.3.1.126 Amaranthus hybridus
-
 ssp. paniculatus (L.) Hejny
-
2.3.1.126 Amaranthus paniculatus
-
 L.
-
2.3.1.126 Amaranthus paniculatus L.
-
 L.
-
2.3.1.126 Amaranthus tricolor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.126 partial Amaranthus cruentus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.126 cotyledon
-
 Amaranthus cruentus
-
2.3.1.126 leaf
-
 Amaranthus cruentus
-
2.3.1.126 seedling
-
 Amaranthus cruentus
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.126 -20°C, protein preparation shows no loss of enzymatic activity when stored for several weeks Amaranthus cruentus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.126 4-coumaroyl-CoA + isocitrate
-
 Amaranthus cruentus CoA + 4-coumaroylisocitrate
-
 ?
2.3.1.126 caffeoyl-CoA + isocitrate
-
 Amaranthus hybridus CoA + 2-caffeoylisocitrate
-
 r
2.3.1.126 caffeoyl-CoA + isocitrate
-
 Amaranthus tricolor CoA + 2-caffeoylisocitrate
-
 r
2.3.1.126 caffeoyl-CoA + isocitrate
-
 Amaranthus cruentus CoA + 2-caffeoylisocitrate
-
 r
2.3.1.126 caffeoyl-CoA + isocitrate
-
 Amaranthus paniculatus CoA + 2-caffeoylisocitrate
-
 r
2.3.1.126 caffeoyl-CoA + isocitrate
-
 Amaranthus paniculatus L. CoA + 2-caffeoylisocitrate
-
 r
2.3.1.126 caffeoyl-CoA + isocitric acid
-
 Amaranthus cruentus CoA + (E)-caffeoylisocitric acid
-
 r
2.3.1.126 feruloyl-CoA + isocitric acid
-
 Amaranthus cruentus CoA + feruloylisocitric acid
-
 r
2.3.1.126 additional information citric, malic tartaric and quinic acids are not accepted as substrates Amaranthus cruentus ?
-
 ?
2.3.1.126 sinapoyl-CoA + isocitric acid minor activity compared to caffeoyl-CoA Amaranthus cruentus CoA + sinapoylisocitric acid
-
 r