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Literature summary extracted from

  • Sathyamoorthy, N.; Takayama, K.
    Purification and characterization of a novel mycolic acid exchange enzyme from Mycobacterium smegmatis (1987), J. Biol. Chem., 262, 13417-13423.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.122 buffer of ionic strength of 0.1 M and above stabilizes Mycolicibacterium smegmatis
2.3.1.122 dithiothreitol, 1 mM, stabilizes Mycolicibacterium smegmatis
2.3.1.122 purification has to be carried out at 4°C Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.122 ACP 0.00056 mg/0.6 ml, 68% reduction of activity Mycolicibacterium smegmatis
2.3.1.122 iodoacetamide 16.7 mM, 53% decrease of activity Mycolicibacterium smegmatis
2.3.1.122 MgCl2 16 mM, 91% inhibition Mycolicibacterium smegmatis
2.3.1.122 Tween 80 strong, might disrupt necessary structure of the TM vesicle/micelle Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.122 0.0083
-
trehalose
-
Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.122 25000
-
SDS-PAGE, gel filtration Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.122 2 alpha,alpha-trehalose 6-mycolate Mycolicibacterium smegmatis
-
alpha,alpha-trehalose + alpha,alpha-trehalose 6,6'-bismycolate
-
r
2.3.1.122 alpha,alpha-trehalose 6-mycolate + alpha,alpha-trehalose Mycolicibacterium smegmatis
-
alpha,alpha-trehalose + alpha,alpha-trehalose 6-mycolate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.122 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.122 ammonium sulfate fractionation, gel filtration and Sephadex column chromatography Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.122 0.00779
-
-
Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.122 2 alpha,alpha-trehalose 6-mycolate
-
Mycolicibacterium smegmatis alpha,alpha-trehalose + alpha,alpha-trehalose 6,6'-bismycolate
-
r
2.3.1.122 alpha,alpha-trehalose 6-mycolate + alpha,alpha-trehalose
-
Mycolicibacterium smegmatis alpha,alpha-trehalose + alpha,alpha-trehalose 6-mycolate
-
r
2.3.1.122 alpha,alpha-trehalose 6-palmitate + trehalose
-
Mycolicibacterium smegmatis alpha,alpha-trehalose + alpha,alpha-trehalose 6-palmitate
-
r
2.3.1.122 additional information exchange of mycolyl group, highly specific for alpha,alpha'-trehalose as the mycolate acceptor, less specific for the acyl donor group Mycolicibacterium smegmatis ?
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.122 monomer 1 * 25000, SDS-PAGE Mycolicibacterium smegmatis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.122 37
-
-
Mycolicibacterium smegmatis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.122 25 55 75% of maximal activity at 25°C, 12% at 55°C Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.122 6.5 7
-
Mycolicibacterium smegmatis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.122 5 9 45% of maximal activity at pH 5.0, 30% of maximal activity at pH 9.0 Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.122 additional information no requirement of cofactors such as ATP or CoA Mycolicibacterium smegmatis