EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.12 | - |
Azotobacter vinelandii |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.1.12 | hanging drop vapor diffusion method | Azotobacter vinelandii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.12 | G610C | 500fold reduced enzymatic activity | Azotobacter vinelandii |
2.3.1.12 | N614D | 9fold reduced enzymatic activity | Azotobacter vinelandii |
2.3.1.12 | S558A | 200fold reduced enzymatic activity | Azotobacter vinelandii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.12 | 0.022 | - |
acetyl-CoA | wild-type enzyme | Azotobacter vinelandii | |
2.3.1.12 | 0.028 | - |
acetyl-CoA | S558A mutant | Azotobacter vinelandii | |
2.3.1.12 | 0.036 | - |
acetyl-CoA | H610C mutant | Azotobacter vinelandii | |
2.3.1.12 | 0.052 | - |
acetyl-CoA | N614D mutant | Azotobacter vinelandii | |
2.3.1.12 | 1.08 | - |
dihydrolipoamide | N614D mutant | Azotobacter vinelandii | |
2.3.1.12 | 5.3 | - |
dihydrolipoamide | H610C mutant | Azotobacter vinelandii | |
2.3.1.12 | 5.37 | - |
dihydrolipoamide | wild-type enzyme | Azotobacter vinelandii | |
2.3.1.12 | 6.4 | - |
dihydrolipoamide | S558A mutant | Azotobacter vinelandii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.12 | dihydrolipoamide + acetyl-CoA | Azotobacter vinelandii | - |
S-acetyldihydrolipoamide + CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.12 | Azotobacter vinelandii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.12 | catalytic domain mutants | Azotobacter vinelandii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.12 | dihydrolipoamide + acetyl-CoA | - |
Azotobacter vinelandii | S-acetyldihydrolipoamide + CoA | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.12 | 0.00417 | - |
acetyl-CoA | with dihydrolipoamide, H610C mutant | Azotobacter vinelandii | |
2.3.1.12 | 0.0143 | - |
acetyl-CoA | with dihydrolipoamide, S558A mutant | Azotobacter vinelandii | |
2.3.1.12 | 0.282 | - |
acetyl-CoA | with dihydrolipoamide, N614D mutant | Azotobacter vinelandii | |
2.3.1.12 | 2.4 | - |
acetyl-CoA | with dihydrolipoamide, wild-type enzyme | Azotobacter vinelandii |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.12 | 6 | 8.4 | stable | Azotobacter vinelandii |