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Literature summary extracted from

  • Sinclair, J.; Delgoda, R.; Noble, M.; Jarmin, S.; Goh, N.; Sim, E.
    Purification, characterization and crystallization of an N-hydroxyarylamine O-acetyltransferase from Salmonella typhimurium (1998), Protein Expr. Purif., 12, 371-380.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.56 expression as His-tagged protein in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.56 sitting and hanging drop methods, 20°C, 10 mg/ml protein solution: 10 mM Tris-HCl, pH 7.9, 0.1 M NaCl, 3 mM dithiothreitol, reservoir solution: 0.8 M Na,K tartrate, 0.1 M MES, pH 7.1, X-ray diffraction Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 sodium potassium tartrate and X-ray diffraction Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.56 0.6
-
 4-aminoveratrole
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.56 1.1
-
 4-anisidine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.56 1.3
-
 4-iodoanisidine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 0.6
-
 4-aminoveratrole
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 1.1
-
 4-anisidine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 1.3
-
 4-iodoaniline
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.118 33570
-
 electrospray mass spectrometry Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.56 Salmonella enterica subsp. enterica serovar Typhimurium
-
 Nho gene
-
2.3.1.118 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.56 recombinant His-tagged protein from E. coli, removal of His-tag Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 single step immobilized metal ion chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.56 0.785
-
 purified enzyme Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.118 0.66
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.56 acetyl-CoA + 4-aminoveratrole
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + N-acetyl-4-aminoveratrole
-
 ?
2.3.1.56 acetyl-CoA + 4-anisidine
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + N-acetyl-4-anisidine
-
 ?
2.3.1.56 acetyl-CoA + 4-iodoaniline
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + N-acetyl-4-iodoaniline
-
 ?
2.3.1.118 acetyl-CoA + 4-aminoveratrole
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + N-acetyl-4-aminoveratrole
-
 ?
2.3.1.118 acetyl-CoA + 4-anisidine
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + N-acetyl-4-anisidine
-
 ?
2.3.1.118 acetyl-CoA + 4-iodoaniline
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + N-acetyl-4-iodoaniline
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.56 37
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.118 18 40 stable for 2 hours Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.56 7.5
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium