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Literature summary extracted from

  • Koester, J.; Bussmann, R.; Barz, W.
    Malonyl-coenzyme A:isoflavone 7-O-glucoside-6-O-malonyltransferase from roots of chick pea (Cicer arietinum L.) (1984), Arch. Biochem. Biophys., 234, 513-521.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.115 most stable in Tris buffer at pH 8, unstable to repeated freezing and thawing, dithioerythritol stabilizes Cicer arietinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.115 0.024
-
 formononetin 7-O-glucoside
-
 Cicer arietinum
2.3.1.115 0.036
-
 Biochanin A-beta-D-glucoside
-
 Cicer arietinum
2.3.1.115 0.048
-
 malonyl-CoA
-
 Cicer arietinum
2.3.1.115 0.054
-
 (-)-maackiain 3-O-beta-D-glucoside
-
 Cicer arietinum
2.3.1.115 0.065
-
 daidzein 7-O-glucoside
-
 Cicer arietinum
2.3.1.115 0.067
-
 kaempferol 7-O-glucoside
-
 Cicer arietinum
2.3.1.115 0.088
-
 genistein 7-O-glucoside
-
 Cicer arietinum
2.3.1.115 0.098
-
 pratensein 7-O-glucoside
-
 Cicer arietinum
2.3.1.115 0.101
-
 succinyl-CoA
-
 Cicer arietinum
2.3.1.115 0.11
-
 2',4,4'-trihydroxychalcone 4'-O-glucoside
-
 Cicer arietinum
2.3.1.115 0.16
-
 apigenin 7-O-glucoside
-
 Cicer arietinum
2.3.1.115 0.335
-
 quercetagetin 7-O-glucoside
-
 Cicer arietinum
2.3.1.115 0.381
-
 quercetin 7-O-glucoside
-
 Cicer arietinum
2.3.1.115 0.46
-
 orobol 7-O-glucoside
-
 Cicer arietinum
2.3.1.115 0.47
-
 luteolin 7-O-glucoside
-
 Cicer arietinum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.115 112000
-
 gel filtration Cicer arietinum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.115 apigenin 7-O-apiosylglucoside + malonyl-CoA Cicer arietinum i.e. apiin apigenin 7-O-apiosylglucoside -6''-O-malonylester + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.115 Cicer arietinum
-
 chick pea
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.115
-
 Cicer arietinum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.115 root 4 days old Cicer arietinum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.115 7.515
-
-
 Cicer arietinum

Storage Stability

EC Number Storage Stability Organism
2.3.1.115 -20°C, 10% glycerol, stable for 4 months Cicer arietinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.115 apigenin 7-O-apiosylglucoside + malonyl-CoA i.e. apiin Cicer arietinum apigenin 7-O-apiosylglucoside -6''-O-malonylester + CoA
-
 ?
2.3.1.115 apigenin 7-O-glucoside + malonyl-CoA
-
 Cicer arietinum CoA + apigenin 7-O-glucoside 6''-O-malonylester
-
 ?
2.3.1.115 luteolin 7-O-glucoside + malonyl-CoA
-
 Cicer arietinum CoA + luteolin 7-O-glucoside 6''-O-malonylester
-
 ?
2.3.1.115 malonyl CoA + genistein 7-O-glucoside
-
 Cicer arietinum CoA + genistein 7-O-glucoside 6''-O-malonylester
-
 ?
2.3.1.115 malonyl-CoA + (-)-maackiain 3-O-beta-D-glucoside
-
 Cicer arietinum CoA + (-)-maackiain 3-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
2.3.1.115 malonyl-CoA + 2',4,4'-trihydroxychalcone 4-O-glucoside
-
 Cicer arietinum CoA + 2',4,4'-trihydroxychalcone 4-O-glucoside 6''-O-malonylester
-
 ?
2.3.1.115 malonyl-CoA + biochanin A 7-O-glucoside
-
 Cicer arietinum CoA + biochanin A 7-O-glucoside-6''-O-malonylester
-
 ?
2.3.1.115 malonyl-CoA + biochanin A-beta-D-glucoside
-
 Cicer arietinum coenzyme A + biochanin A 7-O-(6-O-malonyl-beta-D-glucoside)
-
 ?
2.3.1.115 malonyl-CoA + daidzein 7-O-glucoside
-
 Cicer arietinum CoA + daidzein 7-O-glucoside 6''-O-malonylester
-
 ?
2.3.1.115 malonyl-CoA + formononetin 7-O-glucoside
-
 Cicer arietinum CoA + formononetin 7-O-glucoside-6''-O-malonylester
-
 ?
2.3.1.115 malonyl-CoA + kaempferol 7-O-glucoside
-
 Cicer arietinum CoA + kaempferol 7-O-glucoside 6''-O-malonylester
-
 ?
2.3.1.115 malonyl-CoA + orobol 7-O-glucoside
-
 Cicer arietinum CoA + orobol 7-O-glucoside 6''-O-malonylester
-
 ?
2.3.1.115 malonyl-CoA + pratensein 7-O-glucoside
-
 Cicer arietinum CoA + pratensein 7-O-glucoside 6''-O-malonylester
-
 ?
2.3.1.115 additional information isoflavones with the glucosyl substituent in position 4' don't act as acceptors Cicer arietinum ?
-
 ?
2.3.1.115 additional information succinyl-CoA can replace malonyl-CoA with lower activity Cicer arietinum ?
-
 ?
2.3.1.115 quercetagetin 7-O-glucoside + malonyl-CoA
-
 Cicer arietinum CoA + quercetagetin 7-O-glucoside 6''-O-malonylester
-
 ?
2.3.1.115 quercetin 7-O-glucoside + malonyl-CoA
-
 Cicer arietinum CoA + quercetin 7-O-glucoside 6''-O-malonylester
-
 ?
2.3.1.115 succinyl-CoA + biochanin A-beta-D-glucoside
-
 Cicer arietinum coenzyme A + biochanin A 7-O-(6-O-succinyl-beta-D-glucoside)
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.115 45 50
-
 Cicer arietinum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.115 60
-
 above 2 min, destroyed Cicer arietinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.115 8
-
-
 Cicer arietinum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.115 6.5 9.5 about 50% of activity maximum at pH 6.5 and 9.5 Cicer arietinum