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Literature summary extracted from

  • Matern, U.; Potts, J.R.M.; Hahlbrock, K.
    Two flavonoid-specific malonyltransferases from cell suspension cultures of Petroselinum hortense: partial purification and some properties of malonyl-coenzyme A: flavone/flavonol-7-O-glycoside malonyltransferase and malonyl-coenzyme A: flavonol-3-O-glucoside malonyltransferase (1981), Arch. Biochem. Biophys., 208, 233-241.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.115 0.006
-
 malonyl-CoA
-
 Petroselinum crispum
2.3.1.115 0.01
-
 apiin
-
 Petroselinum crispum
2.3.1.115 0.014
-
 apigenin 7-O-glucoside
-
 Petroselinum crispum
2.3.1.115 0.02
-
 luteolin 7-O-glucoside
-
 Petroselinum crispum
2.3.1.116 0.004
-
 kaempferol 3-O-glucoside
-
 Petroselinum crispum
2.3.1.116 0.005
-
 malonyl-CoA
-
 Petroselinum crispum
2.3.1.116 0.006
-
 isorhamnetin 3-O-glucoside
-
 Petroselinum crispum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.115 soluble
-
 Petroselinum crispum
-
-
2.3.1.116 soluble
-
 Petroselinum crispum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.115 50000
-
 gel filtration Petroselinum crispum
2.3.1.116 50000
-
 gel filtration Petroselinum crispum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.115 apigenin 7-O-apiosylglucoside + malonyl-CoA Petroselinum crispum
-
 apigenin 7-O-apiosylglucoside -6''-O-malonylester + CoA
-
 ?
2.3.1.115 additional information Petroselinum crispum last step in biosynthesis of flavonoid glycosides ?
-
 ?
2.3.1.116 malonyl-CoA + kaempferol 3-O-glucoside Petroselinum crispum
-
 CoA + kaempferol 3-O-glucoside malonylester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.115 Petroselinum crispum
-
 parsley
-
2.3.1.116 Petroselinum crispum
-
 parsley
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.115 glycoprotein
-
 Petroselinum crispum
2.3.1.116 glycoprotein
-
 Petroselinum crispum

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.115
-
 Petroselinum crispum
2.3.1.116
-
 Petroselinum crispum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.115 cell culture
-
 Petroselinum crispum
-
2.3.1.116 cell culture
-
 Petroselinum crispum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.115 additional information
-
-
 Petroselinum crispum
2.3.1.116 additional information
-
-
 Petroselinum crispum

Storage Stability

EC Number Storage Stability Organism
2.3.1.115 -70°C, partially purified enzyme is stable for several months, extensively purified enzyme loses 50% of activity within 2-3 days Petroselinum crispum
2.3.1.116 -70°C, partially purified enzyme is stable for several months, extensively purified enzyme loses 50% of activity within 2-3 days Petroselinum crispum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.115 apigenin 7-O-apiosylglucoside + malonyl-CoA
-
 Petroselinum crispum apigenin 7-O-apiosylglucoside -6''-O-malonylester + CoA
-
 ?
2.3.1.115 apigenin 7-O-glucoside + malonyl-CoA 88% of the activity with apiin Petroselinum crispum CoA + apigenin 7-O-glucoside 6''-O-malonylester
-
 ?
2.3.1.115 apiin + malonyl-CoA i.e. apigenin 7-O-apiosylglucoside, best substrate Petroselinum crispum apigenin 7-O-apiosylglucoside -6''-O-malonylester + malonyl-CoA
-
 ?
2.3.1.115 diosmetin 7-O-glucoside + malonyl-CoA 72% of the activity with apiin Petroselinum crispum CoA + diosmetin 7-O-glucoside 6''-O-malonylester
-
 ?
2.3.1.115 isorhamnetin 3-O-glucoside + malonyl-CoA 11% of the activity with apiin Petroselinum crispum CoA + isorhamnetin 3-O-glucoside 6''-O-malonylester
-
 ?
2.3.1.115 isorhamnetin 7-O-glucoside + malonyl-CoA 12% of the activity with apiin Petroselinum crispum CoA + isorhamnetin 7-O-glucoside 6''-O-malonylester
-
 ?
2.3.1.115 kaempferol 3-O-glucoside + malonyl-CoA 15% of the activity with apiin Petroselinum crispum CoA + kaempferol 3-O-glucoside 6''-O-malonylester
-
 ?
2.3.1.115 luteolin 7-O-glucoside + malonyl-CoA 30% of the activity with apiin Petroselinum crispum CoA + luteolin 7-O-glucoside 6''-O-malonylester
-
 ?
2.3.1.115 additional information last step in biosynthesis of flavonoid glycosides Petroselinum crispum ?
-
 ?
2.3.1.115 naringenin 7-O-glucoside + malonyl-CoA 16% of the activity with apiin Petroselinum crispum CoA + naringenin 7-O-glucoside 6''-O-malonylester
-
 ?
2.3.1.115 quercetin 3-O-glucoside + malonyl-CoA 8% of the activity with apiin Petroselinum crispum CoA + quercetin 3-O-glucoside 6''-O-malonylester
-
 ?
2.3.1.116 malonyl-CoA + isorhamnetin 3-O-glucoside 78% of the activity with kaempferol 3-O-glucoside Petroselinum crispum CoA + isorhamnetin 3-O-glucoside malonylester probably acylated at position 6 of glucose ?
2.3.1.116 malonyl-CoA + kaempferol 3-O-glucoside
-
 Petroselinum crispum CoA + kaempferol 3-O-glucoside malonylester
-
 ?
2.3.1.116 malonyl-CoA + kaempferol 3-O-glucoside
-
 Petroselinum crispum CoA + kaempferol 3-O-glucoside malonylester probably acylated at position 6 of glucose ?
2.3.1.116 malonyl-CoA + quercetin 3-O-glucoside 60% of the activity with kaempferol 3-O-glucoside Petroselinum crispum CoA + quercetin 3-O-glucoside malonylester probably acylated at position 6 of glucose ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.115 8
-
 most active in Tris buffer Petroselinum crispum
2.3.1.116 8
-
 most active in Tris buffer containing bovine serum albumin Petroselinum crispum