Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Matern, U.; Feser, C.; Heller, W.
    N-Malonyltransferases from peanut (1984), Arch. Biochem. Biophys., 235, 218-227.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.112 bovine serum albumin 2 mg/ml, activation Arachis hypogaea
2.3.1.113 bovine serum albumin 2 mg/ml, activation Arachis hypogaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.112 0.005
-
 malonyl-CoA
-
 Arachis hypogaea
2.3.1.112 0.025
-
 D-tryptophan
-
 Arachis hypogaea
2.3.1.113 0.004
-
 malonyl-CoA
-
 Arachis hypogaea
2.3.1.113 0.012
-
 anthranilate
-
 Arachis hypogaea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.112 38000
-
 1 * 38000, SDS-PAGE Arachis hypogaea
2.3.1.112 40000
-
 gel filtration Arachis hypogaea
2.3.1.113 50000
-
 gel filtration Arachis hypogaea
2.3.1.113 51000
-
 1 * 51000, SDS-PAGE Arachis hypogaea
2.3.1.114 45000
-
 gel filtration Arachis hypogaea ssp. fastigiata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.112 malonyl-CoA + D-tryptophan Arachis hypogaea
-
 CoA + N2-malonyl-D-tryptophan
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.112 Arachis hypogaea
-
 peanut, cv. fastigiata (Junagarh-11 or cultivar Shulamit)
-
2.3.1.113 Arachis hypogaea
-
 peanut, cv. fastigiata ("Junagarh-11" or cultivar Shulamit)
-
2.3.1.114 Arachis hypogaea ssp. fastigiata
-
 peanut
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.112 partial Arachis hypogaea
2.3.1.113 partial Arachis hypogaea
2.3.1.114 ammonium sulfate, DEAE-cellulose, Blue Sepharose, Acrylex P-100, Matrix gel green Arachis hypogaea ssp. fastigiata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.112 seedling
-
 Arachis hypogaea
-
2.3.1.113 seedling
-
 Arachis hypogaea
-
2.3.1.114 hypocotyl of young seedlings Arachis hypogaea ssp. fastigiata
-
2.3.1.114 leaf
-
 Arachis hypogaea ssp. fastigiata
-
2.3.1.114 seedling
-
 Arachis hypogaea ssp. fastigiata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.112 0.0002
-
-
 Arachis hypogaea
2.3.1.113 0.00091
-
-
 Arachis hypogaea

Storage Stability

EC Number Storage Stability Organism
2.3.1.112 -20°C, inactivation after 3 months Arachis hypogaea
2.3.1.112 4°C, considerable loss of activity after several weeks Arachis hypogaea
2.3.1.112 glycerol, 10%, stabilizes during storage Arachis hypogaea
2.3.1.113 -20°C, inactivtion after 3 months Arachis hypogaea
2.3.1.113 4°C, considerable loss of activity after several weeks Arachis hypogaea
2.3.1.113 glycerol, 10%, stabilizes during storage Arachis hypogaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.112 malonyl-CoA + 1-aminocyclopropan-1-carboxylic acid condensation at 50% the rate of the condensation with D-tryptophan Arachis hypogaea CoA + malonyl-1-aminocyclopropan-1-carboxylic acid
-
 ?
2.3.1.112 malonyl-CoA + D-tryptophan
-
 Arachis hypogaea CoA + N2-malonyl-D-tryptophan
-
 ?
2.3.1.112 additional information no substrates are L-tryptophan, anthranilate and 3,4-dichloroaniline Arachis hypogaea ?
-
 ?
2.3.1.113 malonyl-CoA + anthranilate
-
 Arachis hypogaea CoA + N-malonylanthranilate
-
 ?
2.3.1.113 additional information no substrates are D-tryptophan and 3,4-dichloroaniline Arachis hypogaea ?
-
 ?
2.3.1.114 malonyl-CoA + 3,4-dichloroaniline
-
 Arachis hypogaea ssp. fastigiata CoA + N-(3,4-dichlorophenyl)-malonamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.112 monomer 1 * 38000, SDS-PAGE Arachis hypogaea
2.3.1.113 monomer 1 * 51000, SDS-PAGE Arachis hypogaea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.112 30
-
 assay at Arachis hypogaea
2.3.1.113 30
-
 assay at Arachis hypogaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.112 8.8
-
 broad Arachis hypogaea
2.3.1.113 8.8
-
 broad Arachis hypogaea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.112 7.5 9 most active Arachis hypogaea
2.3.1.113 7.5 9 most active Arachis hypogaea