Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shima, S.; Weiss, D.S.; Thauer, R.K.
    Formylmethanofuran:tetrahydromethanopterin formyltransferase (Ftr) from the hyperthermophilic Methanopyrus kandleri. Cloning, sequencing and functional expression of the ftr gene and one-step purification of the enzyme overproduced in Escherichia coli (1995), Eur. J. Biochem., 230, 906-913.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.101 expression in Escherichia coli Methanopyrus kandleri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.101 0.04
-
 formylmethanofuran recombinant enzyme Methanopyrus kandleri
2.3.1.101 0.05
-
 formylmethanofuran wild-type enzyme Methanopyrus kandleri
2.3.1.101 0.1
-
 5,6,7,8-tetrahydromethanopterin wild-type enzyme Methanopyrus kandleri
2.3.1.101 0.107
-
 5,6,7,8-tetrahydromethanopterin recombinant enzyme Methanopyrus kandleri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.101 salts optimal salt concentration for activity: 1.5-2.0 M Methanopyrus kandleri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.101 35000
-
 1 * 35000, SDS-PAGE Methanopyrus kandleri

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.101 Methanopyrus kandleri Q49610 strain DSM 6324
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.101 recombinant enzyme Methanopyrus kandleri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.101 additional information
-
-
 Methanopyrus kandleri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
 Methanopyrus kandleri methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.101 monomer 1 * 35000, SDS-PAGE Methanopyrus kandleri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.101 90
-
 recombinant and wild-type enzyme Methanopyrus kandleri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.101 90
-
 stable up to, in presence of salts Methanopyrus kandleri